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Biomedical subjects

M Murugesan

Publications and source records attributed to M Murugesan.

8 recordsLinked to original sources

Direct electron transfer with yeast cells and construction of a mediatorless microbial fuel cell.

The direct electron transfer exhibited by the yeast cells, Hansenula anomala has been demonstrated using the electrochemical technique cyclic voltammetry by immobilizing the microorganisms by two different methods viz., physical adsorption and covalent linkage. The analysis of redox enzymes present in the outer membrane of the microorganisms has been carried out in this work. This paper demonstrates that yeast cells with redox enzymes present in their outer membrane are capable of communicating directly with the electrode surface and contribute to current generation in a mediatorless biofuel cells. The efficiency of current generation has been evaluated using three anode materials.

Bioelectric Energy Sources↗

Formation of multilamellar vesicles ('onions') in peptide based surfactant.

Concentration dependent morphological characteristics of a novel dipeptide derivative Lys-Asp-Lauryl.HBr (1) has been presented. Evidence for "onion" like vesicle formation at higher concentration (>8.2 x 10(-3) M) of peptide (1) in aqueous medium was obtained from conductance and 90 degrees light scattering measurements, and cryo-transmission electron microscopic studies.

Cryoelectron Microscopy↗

Anomalous temperature dependence of peptide films at air-water interface.

The tetrapeptide derivative Tyr-Gly-Phe-Ala-OBz (1) forms monolayers as confirmed by compressibility studies carried out at various temperatures. Peptide 1 monolayer exhibits an anomalous structural transition at 40 degrees C as evidenced by pi-A isotherms recorded at different temperatures. The structural transition is also observed in aqueous solution of trifluoroacetate of peptide 1 as evidenced by fluorescence and Raman scattering intensity measurements.

Air↗

Assessment of growth of Fusarium solani by cyclic voltammetry and possible bioanalytical applications.

Fusarium solani, the fungus isolated from polyurethane waste scraps, was studied for its voltammetric response. The peak current values were obtained for different days and were plotted against time. This electrochemical method based on the voltammetric response showed all the four phases of the growth of the fungus. The growth curve obtained matched well with the conventional methodology, which is obtained by assessing the increase of dry weight of the organisms against time. The electrochemical method is more advantageous than the conventional method because the conventional method is very time consuming and difficult to work with. Further, the electrochemical method clearly shows the decline phase, which is generally not very well defined in the conventional method of assessment of the growth curve. It was confirmed by further experiments that the metabolites were responsible for the anodic peak and not the fungal biomass. Identification of the metabolites that are responsible for the anodic peak is presently being studied.

Biodegradation, Environmental↗