Biomedical subjects

M N MICKELSON

Publications and source records attributed to M N MICKELSON.

10 recordsLinked to original sources

PubMed1964

FUSARIUM GROWTH SUPPORTED BY HYDROCARBONS.

In studies of the microflora associated with fuel storage tanks, a Fusarium species was isolated from No. 1 diesel fuel. The culture was identified as F. moniliforme Sheldon. Attempts were made to cultivate this organism with seven hydrocarbons of 99(+) mole per cent purity as the sole carbon source; growth of the fungal culture was supported only by n-decane and n-dodecane. A spore viability study of F. moniliforme in filter-sterilized diesel fuel with no free water showed that viability was retained for 9 months in this environment.

Alkanes↗

PubMed1964

CHEMICALLY DEFINED MEDIUM FOR GROWTH STREPTOCOCCUS PYOGENES.

Mickelson, M. N. (National Animal Disease Laboratory, Ames, Iowa). Chemically defined medium for growth of Streptococcus pyogenes. J. Bacteriol. 88:158-164. 1964.-Three strains of group A hemolytic streptococci representing three serological types were serially subcultured for an extended period (100 or more transfers) in a peptide-free and protein-free culture medium. An amino acid assay medium, modified by addition of small amounts of glutamine, ammonium acetate, and 0.1 m phosphate (pH 7), was used. In this medium, high concentrations of glutamic acid or glutamine were required, and biotin was stimulatory to growth. Biotin could be partially replaced with NaHCO(3). Maximal growth was obtained with NaHCO(3) when biotin was present, and aspartic acid and asparagine were omitted from the medium. In 24 to 48 hr of incubation at 37 C, luxuriant growth was obtained with complete removal and quantitative fermentation of 1% glucose to lactic acid. Optical densities of cultures were equivalent to those obtained with the best infusion media.

Amino Acids↗

PubMed1960