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Biomedical subjects

M N Singh

Publications and source records attributed to M N Singh.

16 recordsLinked to original sources
PubMed2003

Cloning and sequencing of coat protein gene of an Indian potato leaf roll virus (PLRV) isolate and its similarity with other members of Luteoviridae.

An Indian strain of potato leaf roll virus (PLRV) was purified to generate complementary DNA corresponding to the coat protein (CP) gene. Virus cDNA was synthesized from purified viral RNA using oligo (dT)-anchor primer and virus specific primers. The viral sequence encoding the coat protein was specifically amplified by polymerase chain reaction (PCR), using specific primers bordering the CP gene. The unique amplified product thus obtained was A-T cloned into the pGEM-T Easy vector and the authenticity of the cloned gene verified by dot blot hybridization and sequence analysis. Run-way-transcripts of the cloned CP gene could detect PLRV in tissue imprints and tissue dilution. The nucleotide sequences and the deduced amino acid sequences were compared with the other PLRV isolates and found to be 97-99% identical at both the nucleotide and amino acid sequence level of other isolates. Multiple sequence alignment of deduced amino acid sequences revealed considerable homology to other luteoviruses. A nuclear localization signal located close to the N-terminus of the CP gene was predicted. This is the first report of PLRV coat protein sequence from an Indian strain.

Amino Acid Sequence↗
PubMed2002

Arsenic poisoning in a family.

A family of six members is reported, who accidently consumed arsenic, during preparation of indigenous medicine in their home, and developed arsenic poisoning.

Acute Disease↗
PubMed1998

Kluver Bucy syndrome in young children.

For the first time, Kluver Bucy syndrome (KBS) is described in young children who had no environmental learning of sex. The syndrome has so far been noted only in adults after bilateral temporal lobe affection. A few of its components, especially the hypersexuality and hypermetamorphosis, are likely to manifest differently in very young children. Seven patients in the pre-pubertal age group, who developed KBS as a post-encephalitic sequelae, are described. The patients, 2.5-6 years old, suffered from acute herpes simplex encephalitis (HSE) at 10 months-5.5 years of age and developed KBS on regaining consciousness and ambulation. Altered emotional behaviour, changes in dietary habits, hyperorality and hypersexuality were present in all, while psychic blindness and hypermetamorphosis were noted in a few of the patients. All showed marked indifference and lack of emotional attachment towards their close relatives. Apathy and easy distractibility were rare. Bulimia and strong urge to put non-food items into the mouth were common. Hypersexuality manifested as frequent holding of genitals, intermittent pelvic thrusting movements and rubbing of genitals to the bed on lying prone. Due to lack of environmental learning of sex and also, due to emotional and physical dependence on parents, the manifestations in young children showed modification over those of adults.

Brain Damage, Chronic↗
PubMed1995

Central pontine myelinolysis following 'slow' correction of hyponatremia.

Two patients with central pontine myelinolysis are described for the peculiar mode of development. Both patients were in chronic renal failure and admitted in a stuporous state due to hyponatremia. Both developed central pontine myelinolysis during the hospital stay following slow and judicious correction of hyponatremia. The role of chronicity of hyponatremia prior to its correction, in the genesis of central pontine myelinolysis, particularly in the patients who have chronic debilitating illness, septicemia or malnutrition, is highlighted.

Adult↗
PubMed1993

Motor nerve conduction velocity & Hoffman's reflex latency in newborns.

A total of 72 newborns and their mothers were studied to assess the influence of various variables on the motor nerve conduction velocity (MNCV) and Hoffman's reflex latency (H-RL) of the babies with a view to differentiate fullterm and preterm low birth weight babies. MNCV were significantly lower and H-RL significantly higher in preterm appropriate for gestational (PT-AGA) age babies compared to fullterm appropriate for gestational age (FT-AGA) and fullterm intrauterine growth retarded babies (FT-IUGR). In FT-IUGR babies, only MNCV was significantly reduced as compared to FT-AGA babies.

H-Reflex↗
PubMed1989

Bacterial flora in mothers and babies with reference to causative agent in neonatal septicemia.

One hundred newborns and their mothers were subjected to aerobic bacterial cultures and sensitivity study from different sites. Out of 36 clinically suspected neonatal septicemia cases, the blood culture was positive for bacterial growth in 24 neonates. The incidence of septicemia in PT-AGA, FT-AGA, FT-SFD and postterm-AGA babies was found to be 50.0, 13.5, 28.1 and 50.0%, respectively. The incidence of septicemia in LBW and FT-AGA babies was found to be 45.3 and 13.5%, respectively. The single major isolate in the positive blood culture was Staphylococcus aureus. In Gram positive septicemia the commonest site for colonization of the same organism in newborn was found to be umbilicus followed by nose, throat and external auditory canal, while in Gram negative septicemia rectum and umbilicus were the commonest sites.

Bacteria, Aerobic↗
PubMed1989

Electrophysiological studies (MNCV, H-RL) in full-term newborn babies.

Twenty-five full-term newborns and their mothers constituted study subjects. Mothers were subjected to weight and height measurements, as well as, serum albumin and hemoglobin estimations. Offsprings were subjected to measurements of weight, crown heel length and head circumference along with motor nerve conduction velocity (MNCV) and H-reflex latency (H-RL). The MNCV was found to be significantly lower in growth retarded (FT-IUGR) babies compared to appropriately grown babies (FT-AGA) whereas H-RL was similar in both FT-IUGR and FT-AGA babies.

Electrophysiology↗
PubMedDate not supplied

Electrophysiological studies in newborns with reference to gestation and anthropometry.

Fourteen full term appropriate for gestational age (FT-AGA) and 11 preterm appropriate for gestational age (PT-AGA) newborns and their mothers constituted study subjects. The mothers were subjected to hemoglobin and serum albumin estimations. Offsprings were subjected to measurements of weight, crown heel length and head circumference along with motor nerve conduction velocity (MNCV) and H-reflex latency (H-RL) at right median nerve. The MNCV was found to be significantly lower and H-RL significantly higher in PT-AGA babies compared to FT-AGA babies. The gestation, crown heel length of baby and maternal serum albumin status were found to be significant independent variables. Multiple regression equations have been derived using these parameters as determinative variables. These equations explained much higher per cent variation than explained by simple regression equations.

Action Potentials↗