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Behavioral Research↗
Biomedical subjects
M N Thurston
Publications and source records attributed to M N Thurston.
In vitro thymidine labelling in human and porcine growth plates.
An in vitro technique has been used to label dividing cells in the growth plates of human bones with tritiated thymidine. The patterns of labelling in autoradiographs of human plates are described and values given for the labelling index, the number of cells in the proliferation zones and the heights of hypertrophied cells. In two of the four subjects no labelled cells were found in the growth plates and possible causes for these failures are discussed. In vitro labelling data on five porcine growth plates are also presented for comparison with the human data. In both structure and cell kinetics the epiphyseal cartilage plate in the pig is intermediate between the human and rodent plates.
Adolescent↗
Cell kinetics of growth cartilage of achondroplastic (cn) mice.
Mice homozygous for the recessive gene achondroplasia (cn) aged 16 and 17 days and some homozygotes aged 22-34 days have disruptions in the growth of the proximal tibial growth plate which are due solely to reduced hypertrophic cell height. A second class of homozygote, distinguishable at 22 days, has a greater disruption due to much reduced hypertrophic cell height, reduced labelling index and reduction of the number of cells in the effective proliferative zone.
Achondroplasia↗
Cell kinetics of growth cartilage in spondylo-metaphyseal chondrodysplasia (smc) mice.
The gene for spondylo-metaphyseal chondrodysplasia (smc) in the mouse disrupts the formation of growth plate cartilage. No cartilage columns are found in the head of the tibia and secondary centres of ossification appear very late. The number of cells labelled with tritiated thymidine is sharply reduced at 16, 18 and 21 days of age but hypertrophic cell height is normal. Cells taking up label are found scattered throughout the large cartilaginous epiphyses.
Animals↗
Cell kinetics of growth cartilage in stumpy: a new chondrodystrophic mutant in the mouse.
The proximal growth plates of the tibiae in normal and stumpy mice aged 10-41 days were studied. Autoradiographic studies using tritiated thymidine enabled the size of the proliferating cell population and the labelling indices of the growth plates to be determined. Hypertrophic cell heights were also measured. From these data the overall growth rates for the proximal growth plate of the tibia in normal and stumpy mice were calculated. It was found that the major factor responsible for the reduced growth rate in stumpy up to 21 days was the small hypertrophic cell height, while cell proliferation zone size and labelling indices were of minor importance. Histological observations also revealed a lack of organised endochondral ossification, which worsens with age.
Aging↗