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Biomedical subjects

M Nagamine

Publications and source records attributed to M Nagamine.

At least 73 records · Page 4Linked to original sources
PubMed1982

Successful pregnancy in an acromegalic patient during 2-Br-alpha-ergocryptine (CB-154) therapy.

Foeto-placental function and hormone levels in the maternal, foetal and amniotic compartments have been investigated in an acromegalic woman who was treated with 20 mg bromocriptine/day throughout gestation. Bromocriptine therapy during pregnancy had no effect on urinary oestriol excretion and plasma levels of unconjugated oestriol, progesterone, human placental lactogen, cystine aminopeptidase and heat-stable alkaline phosphatase. The maternal and foetal (cord )blood and amniotic fluid showed prolactin levels of 3.8, 6.5 and 1700 ng/ml, respectively, in the 39th week of pregnancy during bromocriptine therapy. Compared with data from normal pregnancies, these values demonstrated that bromocriptine or an active metabolite crossed the term placenta to suppress prolactin secretion from the foetal pituitary gland and that the prolactin level in amniotic fluid was scarcely affected by the drug. Maternal, foetal and amniotic fluid growth hormone levels were 27.0, 33.0 and 3.8 ng/ml, respectively, thus indicating that dopamine agonists suppress growth hormone only in acromegalic patients, and not in normal babies. Bromocriptine had no effect on thyroid-stimulating hormone concentrations in maternal, foetal and amniotic compartments.

Acromegaly↗
PubMed1980

Purification by affinity chromatography and properties of uroporphyrinogen I synthetase from Chlorella regularis.

Uroporphyrinogen I synthetase (porphobilinogen ammonia-lyase (polymerizing), EC 4.3.1.8) from Chlorella regularis was purified to homogeneity by affinity chromatography on porphobilinogen-AH-Sepharose 4B, which was prepared by reacting carbodiimide with substrate, porphobilinogen. The enzyme was purified 232-fold from the initial crude extract and specific activity was 348 nmol porphyrinogen I formed (mg protein)-1 . h-1 at pH 7.4. The molecular weight of the enzyme was 35 000-36 000 as determined by Sephadex G-100 gel filtration. This enzyme was acidic protein having an isoelectric point of 4.2. The enzyme exhibited a single pH optimum at a pH value of 7.4 both in phosphate and Tris-HCl buffer. The Km value for porphobilinogen was 89 microM as measured by its consumption and 85 microM when uroporphyrin formation was used. The Arrhenius plot obtained from the enzyme activity measurements appeared triphasic with breaks occurring at 35 and 46 degrees C and activation energy was calculated to be 21 700 (10-35 degrees C), 12 700 (35-46 degrees C) and 1800 cal . mol-1 (46-65 degrees C). This enzyme was heat stable and the enzyme still retained 87% of activity, even after 1 h incubation at 75 degrees C.

Amino Acids↗
PubMed1979

[Polyuria and polydipsia in a young boy--a case study (author's transl)].

A 13-year-old boy with a teratoma in the sellar region was presented. This patient had gradually developed polyuria and polydipsia since 2 years prior to the first admission. Endocrinological examination showed a marked reduction of pituitary function. Careful neuroradiological examinations including CT failed to show any mass lesion in the sellar and suprasellar regions but a marked increase of the width of the third ventricle. CSF examination revealed only a slight increase of cell count (lymphocytes). The patient was discharged with hormonal replacement therapy. Seven months later the patient developed visual disturbance and mental disorders. On the second admission, plain skull films showed enlarged sella and CT demonstrated a mass in the suprasellar region. Preoperative diagnosis was suprasellar germinoma. At surgery a teratoma, probably arising from the pituitary fossa, was totally removed. Histopathology of the tumor showed tri-dermal tissues including melanotic progonoma and germinoma. The authors presented various problems of the patient and their solving processes.

Adolescent↗
PubMed1975

Serum alkaline phosphatase isoenzymes linked to immunoglobulin G.

An unusual isoenzyme pattern of serum alkaline phosphatase was found in a patient with skeletal abnormalities due to multiple epiphyseal dysplasia and it was demonstrated that the abnormal pattern was caused by complex formation between serum alkaline phosphatase and immunoglobulin G of the lambda class. Physicochemical studies of the patient's serum alkaline phosphatase showed the properties of an osseous enzyme. Evidence was obtained indicating that the complexing occurred in vitro and that the patient's immunoglobulin G had the ability to bind the hepatic and osseous isoenzymes selectively but not to bile, placental and intestinal isoenzymes. No abnormality was detected in the leucocyte isoenzyme pattern. The relationship between the occurrence of complex formation and the patient's bone disease was not established.

Alkaline Phosphatase↗
PubMedDate not supplied

Syndrome of inappropriate secretion of antidiuretic hormone (SIADH) in a patient with multiple sclerosis.

Association of the syndrome of inappropriate secretion of antidiuretic hormone (SIADH) with multiple sclerosis (MG) is very rare, although many other disorder of the nervous system have been reported to be associated with this syndrome; there is only one case report in the literature. We describe here a patient with the syndrome associated with MS. A 62-year-old women had a variety of neurologic symptoms, where clinical course was typical of MS. Transient episodes of hyponatremia and disturbance of consciousness occurred repeatedly with deterioration of MS. The concentration of antidiuretic hormone (ADH) was high whereas the plasma osmolality was low in the presence of concentrated urine, during the episodes of hyponatremia. Urinary Na excretions exceeded 20 mEq/day. Computed tomography and magnetic resonance imaging revealed lesions in the brain, especially in the periventricular region. The hypothalamus and pituitary appeared normal by these imaging methods. Since the periventricular region surrounds and may be functionally connected to the hypothalamus which plays the central role in the regulation of ADH secretion, it was concluded that association of SIADH and MS in this patient was not coincidental, and that demyelinating processes in the periventricular region exerted an abnormal influence on ADH secretion resulting in SIADH. Contribution of other mechanisms as increased intrathoracic pressure, however, could not be excluded completely.

Cerebral Ventricles↗
PubMedDate not supplied

A case of Graves' disease associated with Klinefelter's syndrome.

Graves' disease was found in a 41-year-old, married male patient with Klinefelter's syndrome. The patient began having finger tremor 5 years previously, and developed palpitation and weight loss 3 months prior to examination. He had a diffuse goiter, exophthalmos, and atrial fibrillation. Plasma levels of T3, T4 and free T4 were 2.8 ng/ml, 16.6 micrograms/dl and 4.5 ng/dl respectively. [123I] uptake was 53%, and TSH receptor antibody was 75%. Although he had no gynecomastia, his general physical appearance was that of typical eunuchoism. Chromosome studies showed a karyotype of 47,XXY. A diagnosis of Graves' disease associated with Klinefelter's syndrome was made.

Adult↗
PubMedDate not supplied

Inhibition of artificial secondary caries in root by fluoride-releasing restorative materials.

This investigation evaluated the fluoride-releasing properties of various fluoride-releasing restorative materials, including resin-modified glass-ionomer cements (Fuji ionomer TypeII LC, Photac-Fil Aplicap, Vitremer), compomers (Ionosit FIL, Compoglass, Dyract) and fluoride-releasing resin composites (Heliomolar radiopaque, Degufill mineral). The study also estimated the effects of those materials on the inhibition of artificial secondary caries around restorations using a bacterial caries-inducing system. The amount of fluoride released from the materials in deionized water was measured every one week for 10 weeks. Class V cavities with the gingival margin located in the root were prepared in extracted human premolars and restored with each of the materials. The restored teeth were incubated in the bacterial artificial caries chamber, and the artificial lesion created around the restoration was observed microradiographically. The resin-modified glass-ionomer cements released the largest amount of fluoride and created a thick radio-opaque zone in the artificial lesion along the restoration-dentin interface. These results indicated that the fluoride-releasing restorative materials have the potential to inhibit secondary caries formation around restorations. Resin-modified glass-ionomer cements presented a particularly strong effect, compared with compomers and fluoride-releasing resin composites.

Acrylic Resins↗