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Biomedical subjects

M Namba

Publications and source records attributed to M Namba.

At least 37 records · Page 2Linked to original sources
PubMed1991

[Effects of suramin on cell proliferation of various types of human malignant cells].

Suramin, a polyanionic compound used clinically for the treatment of African trypanosomiosis and onchocerciasis, has been shown to inhibit the action of various growth factors such as platelet-derived growth factor, epidermal growth factor, fibroblast growth factor and transforming growth factor-beta to stimulate DNA synthesis of cells. Therefore, we investigated effects of suramin on cell proliferation of various types of human malignant cells in culture. Cell lines used were as follows: cervical cancer (HeLa), mammary cancer (MCF-7), bladder cancer (EJ), hepatoma (HuH-7, PLC/PRF/5), embryonal carcinoma (PA-1), and three in vitro transformed human fibroblast lines (KMST-6, SUSM-1, and VA-13). A serum-free defined medium, ASF103, was used when the effect of suramin on proliferation of cells was investigated. This culture medium contains only bovine serum albumin (0.1%), transferrin (5 micrograms/ml) and insulin (5 micrograms/ml) as peptide factors. On day 1, the drug was added to culture medium at the concentration of 25-100 micrograms/ml and 72-96 hr later, the number of cells was counted. The growth inhibition was expressed as the percentage of cells surviving after treatment of cells with suramin, with survival in the control condition representing 100 percent. Proliferation of HuH-7 cells was prominently inhibited and those of PA-1, PLC/PRF/5 and KMST-6 were moderately inhibited under the same conditions of treatment. On the other hand, other five cell lines were not responsive to up to 100 micrograms/ml suramin.

Breast Neoplasms↗
PubMed1990

Cytokeratin expression in human cell lines derived from liver tumors.

Immunohistochemical staining of cell lines derived from human liver tumours showed that five cell lines derived from hepatocellular carcinoma (HCC) and hepatoblastoma were stained positively with monoclonal keratin antibodies, CK-5 (Ker-18-specific) and KL-1 (broad specificity), but not with CK-7 (Ker-7-specific). On the other hand, four carcinoma cell lines derived from the biliary system were stained positively with not only CK-5 and KL-1, but also CK-7.

Albumins↗
PubMed1990

Effects of antioxidants on V79 Chinese hamster cells treated with ferric nitrilotriacetate.

The cytotoxic effects of ferric nitrilotriacetate (Fe-NTA) have been considered to be caused by free radicals produced by the drug. The present study was carried out to determine whether or not cytotoxic effects of Fe-NTA on cell growth and lipoperoxide formation of Chinese hamster cells were reduced by antioxidants. Using a spin trapping technique, we found that hydroxyl radical formation in the cells increased in the presence of Fe-NTA. Antioxidants, with the exception of superoxide dismutase, slightly inhibited production of the hydroxyl radical. Mannitol significantly reduced lipoperoxide formation, but other antioxidants did not. However, the growth inhibitory effects of Fe-NTA were not attenuated by these antioxidants. These results indicated that the cytotoxic effects of Fe-NTA may be mostly due to unknown factors other than oxygen free radicals.

Animals↗
PubMed1979

Creutzfeldt-Jakob disease--alteration in ganglioside sphingosine in the brain of a patient.

Gangliosides isolated from the brain of patients with Creutzfelt-Jakob (C-J) disease were analyzed. The ganglioside current was abnormally low, and the percentage distributions of individual gangliosides and the long-chain base compositions were abnormal. The C20-sphingosine contents of all the ganglioside fractions were low. Abnormalities in ganglioside long-chain bases in adult human brain have been reported previously only in patients with inherited metabolic disorders. These abnormalities in C-J disease seem to be closely related to the cause of the disease.

Brain Chemistry↗
PubMed1979

Ultrastructure of acetylcholinesterase containing striato-nigral neurons transporting HRP retrogradely.

The fine structure of striato-nigral efferent neurons, retrogradely labeled with HRP, was studied. The neuron showed a medium sized ovoid or tri-angular perikaryon. Short or medium lengthened granular endoplasmic reticula, having a tendency to wind and pile up in stacks were observed. This neurons corresponds to a type III neuron according to our classification [4,5]. The neuron showed reaction products, indicating AChE activity, in the granular endoplasmic reticula and plasma membrane.

Acetylcholinesterase↗
PubMed1979

Culture of normal human epidermal cells with 3T3 feeders on millipore filters.

Human epidermal cells were cultured with lethally irradiated 3T3 cells and grew as large colonies both on plastic and millipore filters. Subcultures were possible. The grown colonies were studied morphologically and histologically. The whole intact crossview of epidermal colonies in paraffin sections was obtained from the cell culture on millipore filters. The epidermal cells derived from younger persons had higher efficiency of colony formation than those from older ones. The membrane filter was shown to be good substratum for epidermal cell growth as well as plastic.

Adult↗
PubMed1979

An attempt for improving plating efficiency of human diploid fibroblasts.

An attempt was made to enhance the plating efficiency of normal human diploid skin fibroblasts by testing various culture media. Eagle's minimum essential medium (MEM), Dulbecco's MEM, William's medium E, DM-160, McCoy's 5a, RPMI-1640, Ham's F12, MCDB 102, Waymouth's MB 752/1, and L-15 synthetic media were examined. L-15 was the best among them. Plating efficiency with L-15 supplemented with 20% calf serum ranged from 20 to 60%. Plating efficiency decreased in the order of Eagle's MEM, Dulbecco's MEM, DM-160, and Ham's F12. Waymouth's MB 752/1, MCDB 102 McCoy's 5a, RPMI-1640, and William's medium E were not favorable for high plating efficiency. Although L-15 gave the best plating efficiency and Waymouth's MB 752/1 the worst, the Waymouth medium supported better cell proliferation than L-15, when cell proliferation was compared in mass culture with these two media. It was concluded that L-15 was better suited for culture of small number of cells, and especially for work involving colony formation.

Cell Division↗
PubMed1979

Effect of Nocardia rubra cell-wall skeleton on the induction of lung cancer in ACI/N rats.

Administration of oil-attached Nocardia rubra cell-wall skeleton (N. rubra-CWS) was evaluated for the effect of the induction of lung cancers in ACI/N rats. Lung cancers were induced by 15 weekly intratracheal instillations of 3 mg benzo[a]-pyrene with 3 mg ferric oxide. After the 10th instillation of the carcinogen, rats received seven subcutaneous injections of 100 microgram of N. rubra-CWS at 2-week intervals. In the observation period of 56 weeks, the cumulative incidence of lung cancer was 71.4% in the control group and 48.0% in the N. rubra-CWS treated group. The latent period of tumor induction was prolonged in the group treated with N. rubra-CWS.

Animals↗