[A severe intraoperative complication of cholecystectomy--massive hemorrhage and hemobilia].
Explore the source record for details and available documents.
Aneurysm, Infected↗
Biomedical subjects
M Nasseri
Publications and source records attributed to M Nasseri.
Human papillomavirus type 16 immortalized cervical keratinocytes contain transcripts encoding E6, E7, and E2 initiated at the P97 promoter and express high levels of E7.
Human cervical keratinocytes represent the specific host for the genital human papillomaviruses (HPV). Transfection of these cells with the DNA of a number of the oncogenic HPVs including type 16 was recently shown to result in their immortalization but not in malignant transformation. In this report we show that viral transcripts for E6 and E7 in these cells were as abundant as in cancer derived cell lines. However, in contrast to cancer derived cell lines, immortalized cervical keratinocytes contained RNA with the potential to encode a full-length E2 protein. In addition, the levels of the E7 oncoprotein were at least as high as in cancer derived cell lines, suggesting that E2 interruption, observed in cancer derived cell lines, is not causally related to the high level of E7 expression and, therefore, deregulation of the P97 promoter may not be a prerequisite for HPV-16 associated cancer development. Furthermore, we show that E6, E7, and E2 encoding transcripts all originate from the viral promoter, P97. Unlike in cancer derived cell lines, all transcripts terminated at the early poly(A) site.
Blotting, Northern↗
Genetic analysis of CRPV pathogenesis: the L1 open reading frame is dispensable for cellular transformation but is required for papilloma formation.
Genetic studies to elucidate the role of papillomaviruses in the development and progression of tumors have been severely hampered because the viruses cannot be grown in tissue culture and therefore mutants are not available. We have employed recombinant DNA for papilloma induction to identify essential sequences involved in papillomavirus pathogenesis. Here, we demonstrated that deleting most of the open reading frame (ORF) L2 did not affect the potential of viral cottontail rabbit papillomavirus (CRPV) DNA to induce papillomas. The extrachromosomally maintained DNA in the papillomas was not rearranged and the major transcripts of 1.3 and 2.0 kb encoded E7 and E6, respectively. A recombinant DNA containing a larger deletion lacking the 3' terminal half of ORF L2 and all of ORF L1 (pdlBc/l) did not induce papillomas. The results indicate that sequences in the late region not required for transformation of NIH 3T3 cells by bovine papillomavirus type-1 essential in CRPV for induction of papillomas.
Animals↗
The cleavage recognition signal is contained within sequences surrounding an a-a junction in herpes simplex virus DNA.
Herpesvirus genome maturation involves site-specific cleavage of viral DNA concatemers and encapsidation of unit-length molecules, processes that are apparently coupled. Here, applying a transfection-infection approach, we have investigated the arrangement of the DNA sequence elements involved in cleavage and shown that specific cleavage occurs independently of DNA replication. We show that the cis-acting signal for cleavage is located within a 179-bp fragment from across an a-a junction formed as part of the genome maturation process of herpes simplex virus 1. Plasmids carrying the 179-bp fragment are cleaved at the appropriate site even though they are unable to replicate in HSV-infected cells. When linked to an origin, the same 179-bp a-a fragment will replicate and package into progeny virus as a defective genome. Two highly conserved homologies, pac1 and pac2, that have been observed in all herpesviruses examined, including cytomegalovirus, Epstein-Barr virus, varicella-zoster virus, and herpes simplex virus 2 as well as the herpes simplex virus 1 genome, are contained within the 179-bp fragment. This suggests that a common mechanism is utilized for genome maturation in the herpesvirus group.
Animals↗
A variant of CRPV DNA preferentially maintained as a plasmid in NIH 3T3 cells and characterization of its transcripts in nude mouse tumors.
Rabbit and human papillomaviruses are strictly epitheliotropic and their DNA replicates extrachromosomally in benign lesions (warts) of their natural host. Their tissue and host specificity is thought to be genetically controlled and may account for the inefficiency in transforming heterologous cells. In our hands, CRPV DNA did not induce foci in NIH 3T3 cells and in cotransfections with a selectable marker only integrated DNA was found. The viral DNA appeared to be transcriptionally inactive since no transcripts could be detected and in cells were not tumorigenic for nude mice. In contrast to these results a spontaneously derived CRPV variant DNA was able to replicate extrachromosomally and a majority of focus-derived cell lines were tumorigenic for nude mice. In nude mouse tumors the variant DNA remained exclusively extrachromosomal and viral transcripts were detected. The sizes of the major transcripts were 2.0 and 1.3 kb and this suggested that E6 and E7 were expressed. The situation in rabbit tissue was different; both variant and wild-type DNA were maintained extrachromosomally. The extrachromosomal maintenance of the variant but not of wild-type DNA in mouse cells suggests that the variant lacks sequences which may play a role in the host and the tissue restriction of CRPV. The deletion in the variant DNA was located in the late region and included most or all of L1 and a carboxy terminal segment of L2. A second deletion eliminated some pBR322 sequences.
Animals↗
Identification of the major cottontail rabbit papillomavirus late RNA cap site and mapping and quantitation of an E2 and minor E6 coding mRNA in papillomas and carcinomas.
The capsite of the 2.6- and 4.8-kb major late transcripts of cottontail rabbit papillomavirus (CRPV) has been mapped by primer extension. A leader exon of about 300 nucleotides common to both RNAs is located in the untranslated region of the genome upstream of the capsites for early transcripts. In contrast to the early capsites which are all preceded by TATA boxes, no such sequence is present 30 nucleotides upstream of the late capsite. These data indicate that the switch from early to late transcription involves recognition of a new promoter and suppression of transcription termination at the early polyadenylation site. We have also identified a minor exon with a coding potential for a putative E2 transactivating protein. Quantitation by S1 mapping of the E2 coding exon and a minor exon coding for a full-sized E6 protein unique in size to the highly oncogenic CRPV did not reveal differences in the level of transcription between papillomas and carcinomas.
Animals↗
A human papilloma virus type 11 transcript encoding an E1--E4 protein.
The human papilloma virus (HPV) associated with a genital wart (condyloma acuminatum) was determined to be type 11. The majority of the viral DNA molecules were monomeric circles present in the cells at high copy number, as demonstrated by one- and two-dimensional agarose gell electrophoretic separation followed by Southern blot analysis. A cDNA library in phage lambda gt11 was constructed from poly(A)-selected mRNA recovered from the tissue. Recombinant clones corresponding to the most abundant 1.2-kb viral mRNA species detected by Northern blot hybridization and by electron microscopic analysis of R loops were isolated and their nucleotide sequence was determined. Comparison to the prototype HPV-11 DNA sequence revealed that this message consisted of two exons. The promotor-proximal exon spanned nucleotides 716 through 847 and the distal exon included nucleotides 3325 through 4390 or 4392. The mRNAs were alternatively polyadenylated after either of these latter two sites, in both cases following a G and preceding a U residue. Fourteen or sixteen bases upstream from the poly(A) was the hexanucleotide AGUAAA, which apparently serves as the signal for cleavage and polyadenylation of the nascent message. The splice donor and acceptor sites conformed to the usual /GU. . .AG/pattern. The exons joined open reading frame (ORF) E1, which contributed the initiation codon and four additional triplets, to ORF E4, which specified 85 amino acids to encode a protein of 10,022 Da. The cDNA also contained the ORFs E5a and E5b toward the 3' end. The complete sequence of the cDNA revealed three single-base changes from the prototype HPV-11, two resulting in altered amino acids in E4. Neither affects the coding potential of the overlapping E2 ORF. The function of the E1--E4 protein is unknown.
Base Sequence↗
Human papillomavirus types 6 and 11 mRNAs from genital condylomata acuminata.
We have identified and mapped a number of RNA species of human papillomavirus types 6 and 11 from condylomata acuminata by the electron microscopic R-loop technique. Each of the early (E)- and late (L)-region open reading frames (ORFs) deduced from the DNA sequences was represented in one or more transcripts. In addition, RNA species that could encode the modulator of DNA replication and the repressor of transcription, functions recently identified in the genetically similar bovine papillomavirus type 1, were also detected. Some ORFs were 5' proximal in one or more transcripts, whereas others were not 5' proximal in any species, suggesting that internal initiation of translation might be required to gain access to these latter ORFs. Virtually all transcripts had their 5' ends located in the E region and were polyadenylated at one of two sites, i.e., at the end of the E region or at the end of the L region. The great majority of the RNAs were derived from the E region of the genome, with one species approximately 50 to 100 times more abundant than the others. For most of the RNAs, the 5' end mapped near nucleotide 700; minor populations had 5' ends near nucleotide 100 or 1200. By correlating our mapping data with the genomic DNA sequences as well as available RNA structures and cDNA sequences of several papillomaviruses, we predict a number of mRNA splice donor and acceptor sites and suggest that the papillomaviruses have sophisticated usage of ORFs through alternative promoters, mRNA splice sites, and polyadenylation sites.
Anus Neoplasms↗
Generalized infection and lack of delayed hypersensitivity in BALB/c mice infected with Leishmania tropica major.
The susceptibility of a few strains of mice to a subcutaneous injection of Leishmania tropica major, the causative agent of cutaneous leishmaniasis in humans, was studied. The infection in six strains (CBA, AKR/J, AKR/cu, C57BL/6, A/J, and C3H) remained cutaneous, and the animals recovered within 3 to 4 months. In contast, the infection in BALB/c became generalized and killed 1005 of infected animals. Intraperitoneal injection of infected liver of BALB/c to A/J and syngeneic mice produced a lethal disease in BALB/c but no infection in A/J mice. Lower doses of the parasite produced a lethal infection in BALB/c but no apparent disease in A/J. Hence, the host rather than the parasite is responsible for the outcome of the disease. The peak antibody titer of BALB/c mice was not significantly higher than that of A/J mice. However, BALB/c failed to show any delayed hypersensitivity to leishmania tested by footpad reaction, whereas A/J mice showed a strong response.
Animals↗
[Effects of bacterial peritonitis on the low-pressure system in man].
The effective compliance (C) of the "low-pressure system" has been measured in ten patients with bacterial peritonitis by means of plotting pressure and volume on the first postoperative day (deltaV = 500 ml/10 min). The value of C is decreased in one group of patients, while it increases to as much as five times that of "normal patients" in the other. The cause might be a summation effect of hypovolemia, blood volume shift from intra- to extrathoracic space, and endotoxin reaction on the tone of vascular smooth muscle. The augmentation of the intravascular volume results in an increase of portal venous pressure, which is less than central venous pressure. Splanchnic pooling through increased vascular resistance does not appear.
Bacterial Infections↗
[Varied circulatory effects after rapid infusion of hydroxyethyl starch, dextran 60 and blood in the postoperative patient].
The acute hemodynamic effects which occur after a quick infusion (500 ml per 10 minutes) of hydroxyethyl starch, dextran 60 or autologous blood with identical hematocrit were studied in three groups of 10 patients each on the first day after laparotomy. If blood is infused there is only a moderate increase of the total perfusion (+18%) observed, however hydroxyethyl starch or dextran 60 infusions result in a striking increase of the cardiac output (+34%, +44%, respectively). There is no significant difference among the latter two groups. Thus, hydroxyethyl starch as well as dextran 60 can be considered as a potent volume substitute, if an acute effectiveness is wanted.
Blood Transfusion, Autologous↗
[Postoperative peritonitis. A contribution on diagnosis].
Symptoms and signs of 18 patients with postoperative bacterial peritonitis were compared with the findings in uncomplicated postoperative cases. Their diagnostic values were examined: 1. Elevated temperature, hyperventilation, and somnolence are relevant, being indicative of bacterial peritonitis and already occurring before the typical findings on abdominal examination. 2. In those patients with peritonitis, hemodynamics, and metabolism are characterized by hyperdynamic circulation, premature arterial hypotension with dry warm skin, and lactate accumulation. 3. Laboratory data often reveal thrombocytemia, leucocytosis with shift to the left, and a relative and absolute hypophosphatemia.
Blood Circulation↗
[Aortic arch syndrome (author's transl)].
This report demonstrates a case of a fully developed aortic arch syndrome with an unusual lack of symptoms. A sufficient collateralisation and nearly free cerebral vessels were hiding the clinical manifestations of arteriosclerosis for a long time. Besides the diagnostic maneuvers the operative therapy is shown during which a short, acute circulatory failure occurred caused by a reflex reaction of the carotic sinus due to the improved blood supply.
Aortic Arch Syndromes↗
Comparative studies on the effect of oxygen high pressure (OHP) on different species; with special reference to the organ preservation.
Different species of animals (warm-blooded, poikilotherms and cold-blooded) were exposed to oxygen high pressure (OHP) (1-4 ata) for 2-8 h. OHP revealed (a) a different vulnerability in various species; (b) an inverse correlation between basal oxygen consumption and their vulnerability; (c) the critical point of the toxic effect of OHP in normothermia was an exposition time of 2 h with 2 ata OHP; (d) morphological changes of lung, CNS and vessels -- intense folding of alveolar septa with cell hyperplasia and hypertrophy; (e) electron-microscopical demonstration of damaged endothelial cells with total or partial occlusion of the capillaries.
Animals↗
[Behavior of vasomotor tonus after lung autotransplantation].
In eight lung autotransplants and six normal lungs the vasomotor activity was tested with norepinephrine and acetylcholine. In autotransplants and normal lungs both substances elicit the same effects. The long-term interruption of the central innervation does not provoke a vasoconstriction in lung autotransplants.
Acetylcholine↗
[Retroperitoneal hemangiopericytoma].
Explore the source record for details and available documents.
Female↗
[Pressures, blood gases, pH, lactate and pyruvate concentrations in the portal venous blood in patients after laparotomy during the first 9 days (author's transl)].
In 24 patients (16 women, 8 men, mean age 60 years), who underwent abdominal operations and who had a uncomplicated postoperative course, a teflon-tube was inserted into the portal vein at the end of laparotomy and remained there for maximal 9 days. The mean values of the portal venous pressure as well as of the portal-central venous pressure gradient are unchanged during the period of all 9 days and are between 7,4 and 7,9 mmHg, between 5,6 and 6,9 mmHg respectively. The arterio-portal venous 0(2)-content difference shows in the mean no fluctuations and is about 2 Vol.%. On the first postoperative day the lactic and pyruvic acid concentrations in the artery and portal vein are moderately, but significantly elevated and decrease to normal values until day 7. - 9.
Adult↗
[New methods of circulatory support: cardiac transplant as auxiliary pump (author's transl)].
A simple method of dystopic cardiac transplantation as a left-heart bypass was experimentally studied and details of the method are described. The hemodynamic investigation of this biological assisted circulation on the normal and failing heart, which was induced by subtotal occlusion of the asc. aorta, as well as its longtime support of the chronically damaged left myocardium showed the efficacy of this method. The advantages of this biological method in comparison to that of mechanical devices and orthotopic cardiac transplantation are discussed.
Angiography↗