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Biomedical subjects

M Nicolò

Publications and source records attributed to M Nicolò.

At least 19 recordsLinked to original sources
PubMed2000

Detection of tenascin-C in surgically excised choroidal neovascular membranes.

BACKGROUND: Increase of tenascin-C (TN-C) expression has been found in pathologic tissues in which angiogenesis occurs. The aim of this study was to investigate TN-C expression in human choroidal neovascularization (CNV). METHODS: Ten choroidal neovascular membranes were surgically removed from 10 patients with age-related macular degeneration (n=6) and multifocal choroiditis (n=4). All membranes underwent immunohistochemical evaluation using monoclonal antibodies against TN-C and factor VIII. RESULTS: All membranes were positive for TN-C, which was abundantly and diffusely expressed in the extracellular matrix. CONCLUSIONS: Our results support the concept that TN-C has a role in cell proliferation and neovascularization in humans. TN-C, as a marker of angiogenesis, may provide novel rationales for the development of pharmacologic therapies for neovascular disorders, particularly CNV.

Biomarkers↗
PubMed1999

Morphological, physiological, and biochemical changes in rhodopsin knockout mice.

Mutations in rod opsin, the visual pigment protein of rod photoreceptors, account for approximately 15% of all inherited human retinal degenerations. However, the physiological and molecular events underlying the disease process are not well understood. One approach to this question has been to study transgenic mice expressing opsin genes containing defined mutations. A caveat of this approach is that even the overexpression of normal opsin leads to photoreceptor cell degeneration. To overcome the problem, we have reduced or eliminated endogenous rod opsin content by targeted gene disruption. Retinas in mice lacking both opsin alleles initially developed normally, except that rod outer segments failed to form. Within months of birth, photoreceptor cells degenerated completely. Retinas from mice with a single copy of the opsin gene developed normally, and rods elaborated outer segments of normal size but with half the normal complement of rhodopsin. Photoreceptor cells in these retinas also degenerated but did so over a much slower time course. Physiological and biochemical experiments showed that rods from mice with a single opsin gene were approximately 50% less sensitive to light, had accelerated flash-response kinetics, and contained approximately 50% more phosducin than wild-type controls.

Animals↗
PubMed1999

Follicular large-cell lymphoma of the orbit: a clinicopathologic, immunohistochemical and molecular genetic description of one case.

BACKGROUND: Follicular large cell lymphoma of the orbit is a very rare and aggressive lymphoproliferative disease of the ocular adnexa. In this study we analyzed the clinicopathologic characteristics of one patient, including the immunoglobulin gene rearrangement assay by means of polymerase chain reaction. CASE REPORT: A 71-year-old female underwent an incisional biopsy in the superior nasal quadrant of the left orbit following the occurrence of bilateral eyelid edema and ptosis. Histology and immunohistochemistry revealed a follicular large cell lymphoma of the orbit. Computerized tomography aimed at staging the disease revealed a supraclavicular lymphoadenopathy approximately 0.5 cm in diameter. Bone marrow biopsy was negative for lymphoma. The patient was classified as stage IV. She underwent a 10-week cycle of polychemotherapy (VP16, adriamycin, cyclophosphamide, vincristine, prednisone, bleomycin). Ten months after therapy, disease recurred at the cervical lymph nodes and the patient underwent a radiotherapy cycle of 4320 cGy with disease remission. At the time of writing, the patient presents a left axillary adenopathy on computerized tomography followup. CONCLUSION: Ophthalmologists should be aware of possible rare occurrences of follicular large cell lymphomas of the orbit. In this case the clinical outcome is always more aggressive than the more common MALT-type lymphoma, which usually exhibits benign behavior.

Aged↗
PubMed1998

Marker gene transfer and oncolysis of human Y79 retinoblastoma cells mediated by herpes simplex virus mutants.

Three different herpes simplex virus (HSV) mutants, designated hrR3, MGH-1 and R3616, were used to infect Y79 retinoblastoma cells grown in suspension. Two parameters were assayed: (a) vector-mediated gene expression, measured by histochemical staining of a transferred LacZ transgene, and (b) virus-mediated oncolysis, determined by the inability of infected cells to exclude trypan blue dye. The tested HSV mutants were found to infect cells grown in suspension at a relatively low multiplicity of infection (MOI = 0.01) and were capable of transferring the LacZ gene as early as 2 days after infection. Furthermore, differences in oncolytic activity were observed amongst the tested viruses: MGH-1 and R3616 exhibited 50% cell kill at a MOI of 0.1 over a period of 6 days, whereas hrR3-mediated oncolysis appeared less efficient. These studies provide a rationale for further exploitation of oncolytic herpes viruses as a potential treatment of intraocular tumors.

Cell Death↗
PubMed1991

[The role of phagocytic cells in periodontal disease].

Having described the morphological and functional characteristics of phagocytic cells, the paper underlines that, in addition to the etiological responsibility of bacterial plaque, the main role in the onset and evolution of periodontitis is played by the host's response. Phagocytic response plays a fundamental role in the host's defence reaction and represents the first barrier to the penetration of bacteria into periodontal tissue. In addition to their defensive role, phagocytic cells may also be responsible for damage to periodontal tissue as a collateral effect of their phagocytic function, thus worsening the periodontal lesion.

Granulocytes↗
PubMed1989

[Periodontitis associated with systemic diseases with qualitative deficiency of phagocyte function. II. Down's syndrome].

Numerous systemic syndromes with different aetiopathogenetic and clinical features are constantly accompanied by functional changes in polymorphonucleated neutrophils and, particularly, in their chemotactic properties. The function they perform, namely impeding invasion of the organism by external aggressive factors, cannot therefore be implemented. These patients are thus abnormally susceptible to infections and present a high frequency of serious periodontal disease.

Down Syndrome↗
PubMed1989

[Periodontitis associated with systemic diseases with qualitative deficiency of phagocyte function. III. Papillon-Lefevre syndrome].

Numerous systemic syndromes with different aetiopathogenetic and clinical features are constantly accompanied by function changes in neutrophil polymorphoanucleates and, particularly, in their chemotactic properties. The function they perform, namely impeding the invasion of the organism on the part of aggressive external factors, cannot therefore be implemented. These patients are thus abnormally susceptible to infections and present severe periodontal disease with high frequency.

Humans↗
PubMed1989

[Desquamative gingivitis: etiopathogenetic and clinical assessment].

The aetiopathogenetic, diagnostic, clinical and therapeutic aspects of a particular form of gingival condition given various names in the past but presently defined as "chronic desquamative gingivitis" have been examined. Desquamative gingivitis is an uncommon clinical condition whose aetiology remains uncertain. It has therefore not yet been definitely established wether it is a specific entity or whether, as is confirmed by much clinical evidence and laboratory data, it is merely an oral clinical sign of dermatological type pathologies (pemphigus vulgaris, benign mucosal pemphigoid, bullous pemphigoid, erosive lichen ruber planus, psoriasis).

Gingival Diseases↗
PubMed1989

[Periodontitis associated with systemic diseases with qualitative deficiency of phagocyte function. I. Diabetes mellitus].

Diabetes mellitus is accompanied constantly by functional changes in polymorphonucleated neutrophils and particularly in their chemotactic properties. Their function of impeding the invasion of the organism on the part of aggressive external factors cannot therefore be implemented. These patients are, therefore abnormally susceptible to infection and, with high frequency, also present serious periodontal disease.

Diabetes Complications↗
PubMed1989

[Evaluation of the therapeutic efficacy and tolerance of a new thiamine compound (benzoyloxymethylthiamine) in the treatment of postoperative pain symptomatology in odontostomatological surgery].

The therapeutic effectiveness of benzoyloxymethyl-thiamin in controlling post-operative pain in odontostomatological surgery has been examined. The double blind study used a placebo and concerned 100 patients subjected to avulsion of the third molar in dysodontiasis on an out-patient basis. The results point to the effectiveness of benzoyloxymethyl-thiamin in the symptomatic treatment of post-operative pain symptomatology both as regards its duration and intensity; the drug also proved to be very well tolerated and its administration was not accompanied by any unwanted side-effects.

Adult↗
PubMed1989

[Burning mouth syndrome].

The burning mouth syndrome is a pathology characterized by burning oral mucoses. The etiological factors can be numerous. The Authors suggest a nosologic classification of this disease.

Burning Mouth Syndrome↗
PubMedDate not supplied

Malignant melanoma of soft tissues (clear cell sarcoma) of the foot. Is MRI able to perform a specific diagnosis? Report of one case and review of the radiological literature.

The magnetic resonance (MR) findings in malignant melanoma of soft tissues, also called clear cell sarcoma of tendons and aponeuroses, have been described as a focal abnormality with a specific MR pattern of increased signal intensity (relative to normal muscle) on T1 weighted sequences and variably decreased signal intensity on T2 weighted sequences (relative to surrounding fat). We have reported here a case of malignant melanoma of soft tissues of the foot, studied with ultrasonography (US) and MR, in which MR showed T1-hypointensity, T2-hyperintensity and marked gadolinium uptake. We have described the relationship between the intracytoplasmic melanin amount of and these atypical MR findings.

Adolescent↗
PubMedDate not supplied

[Aphthosis].

Explore the source record for details and available documents.

Anti-Bacterial Agents↗