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Biomedical subjects

M Niemi

Publications and source records attributed to M Niemi.

At least 19 recordsLinked to original sources
PubMed1989

Bacteriological studies of tuberculosis in Sidamo Regional Hospital.

All of the 104 strains isolated from tuberculosis patients in Sidamo Regional Hospital were identified as M. tuberculosis. Primary single drug resistance to isoniazid and streptomycin was represented by two strains in each case. Four strains showed double drug resistance to the same drugs. None were resistant to thiacetazone, rifampicin and ethambutol.

Bacteriological Techniques↗
PubMed1989

Increased non-enzymatic glycosylation and reduced solubility of skin collagen in insulin-dependent diabetic patients.

The solubility of skin collagen into acetic acid and by pepsin digestion and the degree of non-enzymatic glycosylation of collagen (ketoamine linkage) in these fractions was determined in skin specimens from 27 insulin-dependent diabetic subjects and from 17 age-matched controls. Glycosylation in acid soluble collagen specimen was significantly increased in the diabetics, 1.9 +/- 1.8 (SD)ng of hexose/micrograms of hydroxyproline in comparison to the controls. 0.9 +/- 0.8 ng of hexose/micrograms of hydroxyproline. No significant difference in this respect was noted in pepsin soluble collagen specimens. The solubility of collagen into acetic acid and by pepsin digestion were significantly reduced in the diabetics. No clear relationships between non-enzymatic glycosylation or collagen solubility and diabetic late complications (nephropathy, retinopathy or limited joint mobility) were noted. We suggest (a) that equilibrium levels of early glycosylation products are different in acid and pepsin soluble collagen specimens. (b) ketoamine linkage glycosylation products by themselves are not directly involved in diabetic late complications and (c) the solubility in acid and digestibility of collagen by pepsin may be an indicator, even though nonspecific, of increased amounts of advanced glycosylation end products.

Collagen↗
PubMed1989

Sensitivity patterns of bacteria isolated from patients at Sidamo Regional Hospital.

This study from the Sidamo Regional Hospital presents the prevailing sensitivity to common antimicrobials among 337 bacterial isolates from abscesses, infected wounds, urine or gonorrhoeal infections. As expected, Staphylococcus aureus Escherichia cloi, Proteus supp and Streptococcus pyogenes were most frequently grown from abscesses and infected wounds, whereas E. coli, Proteus spp, Klebsiella spp, and coagulase negative staphylococci dominated in urinary tract infections. Very high rates of reduced sensitivity, including multiresistance to commonly used antibiotics, were detected. Of 54 isolates of Niesseria gonorrhoeae 84% were resistant to penicillin-G by disc diffusion method. Beta lactamase production was detected in 4 of 6 isolates tested.

Abscess↗
PubMed1987

Immunohistochemical identification of macrophages, lymphoid cells and HLA antigens in the human testis.

The presence and distribution of macrophages, several subtypes of lymphocytes and HLA antigens in the human testis were studied using monoclonal antibodies and immunocytochemical staining. Cells identified as tissue macrophages were relatively abundant. They were dispersed individually in the interstitium and were often included in Leydig cell clusters. Occasional macrophages were observed in the tubular wall, but never in the seminiferous tubules. Very few lymphocytes were found in the testicular interstitium. These were mainly T lymphocytes. T helper/inducer and T cytotoxic/suppressor cells were extremely scarce. B1 + cells were not observed, but occasional Leu-14+ cells could be seen. NK cells were not found. The number of T lymphocytes in the tunica albuginea was considerably higher than in the interstitial tissue. HLA-ABC and HLA-DR antigens could be observed in the endothelial cells and macrophages which were both strongly positive. The myoid cells and the Leydig cells expressed these antigens only weakly. Neither of the HLA antigens was expressed in the germinal epithelium.

HLA Antigens↗
PubMed1986

Macrophages in the interstitial tissue of the rat testis.

Macrophages were identified in the intertubular tissue of the rat testis by loading animals with a particulate vital dye (trypan blue or India ink) and by localizing immunocytochemically a macrophage membrane antigen (MRC W3/25). Leydig cells were identified by the histochemical staining reaction for 3 beta-hydroxysteroid dehydrogenase activity and by a monoclonal antibody. Macrophages were scattered in the interstitial tissue closely attached to and mixed with the Leydig cells. They were never found in the seminiferous tubules. The macrophages comprised about 25% of all the cells in the interstitium. Double staining with a vital dye and a marker antibody showed that all the phagocytosing cells were macrophages and that the Leydig cells did not take up vital dyes. Double staining for the demonstration of the 3 beta-hydroxysteroid dehydrogenase activity and the macrophage antigen likewise revealed two distinctly different cell populations. Crude Leydig cell preparations obtained by collagenase treatment of the testis contained macrophages (12-14%). Macrophages were present throughout the postnatal prepuberal development of the testis. Their density was increased in the cryptorchid and irradiated testis.

3-Hydroxysteroid Dehydrogenases↗
PubMed1986

Gamma glutamyl transpeptidase in the vasculature of the rat testis.

Activity of gamma glutamyl transpeptidase (GGT) in the testes of mature and prepuberal rats was investigated histochemically and biochemically. Histochemically, the enzyme activity was localized predominantly in the arterial and arteriolar endothelium and was absent from the capillaries and the seminiferous tubules. The activity in the arterial endothelium extended to the testicular artery on the surface of the testis and in the spermatic cord, but the veins in both the pampiniform plexus and on the testicular surface were negative. The endothelium of the testicular artery was already faintly positive at birth, and the activity increased during the second and third postnatal week during the branching and remodeling of the intratesticular arteries and arterioles. Activity of GGT was estimated quantitatively after dissection of the testis into tubular and interstitial tissue. The enzyme activity was very low in the tubules. It was fivefold stronger in the interstitium, and this activity was further enhanced by pretreatment of the dissected tissue with collagenase to remove the Leydig cells.

Animals↗
PubMed1983

Antibiotic resistance among different species of fecal coliforms isolated from water samples.

The distribution of resistance to ampicillin, chloramphenicol, sulfonamides, tetracycline, and streptomycin among fecal coliforms in sewage, surface waters, and sea water was investigated. The incidence of resistant strains among isolates varied significantly among the water samples, without obvious connection with the water source or the level of pollution. The average frequency of multiple resistance was not always high in the same samples in which the overall resistance was high. The species composition varied considerably in different water samples. A significant correlation was observed between the relative frequency of Klebsiella species and the incidence of ampicillin resistance in water samples. The importance of species composition of fecal coliforms, affected by their source and by the aquatic environment, on the resistance pattern is noted.

Anti-Bacterial Agents↗
PubMed1975

DNA and protein synthesis of prostatic cultures in relation to histological response under the influence of testosterone and its metabolites.

Testosterone as well as dihydrotestosterone and 5alpha-androstane-3beta, 17beta-diol were capable of maintaining, in rather low concentrations (0.02, 0.02 and 1.0 mug/ml, respectively), the histological structure of the rat ventral prostate in organotypic tissue culture. By means of histometrical analysis testosterone and dihydrotestosterone were found to be active in lower concentration (0.05 mug/ml) than adrostanediol. In addition to the visual evaluation of the structure of the explants their total DNA and protein content was measured as well as their rate of incorporation of tritiated thymidine and leucine. In a concentration of 1 mug/ml the response of the ventral prostate culture was almost equal to all the steroids. Androstanediol, however, caused no stimulation in lowest concentrations (0.02-0.1 mug/ml) and was not toxic in the highest concentrations (100 mug/ml) at which testosterone and dihydrotestosterone were definately cytolytic. When the serum from the culture medium was omitted even hormone concentrations close to the physiological level (0.003 mug/ml) could stimulate thymidine and leucine incorporation into the cultured ventral prostate.

Androstanes↗
PubMed1975

The effects of testosterone administered neonatally on the incorporation of 3H-leucine into protein by thyroid gland of rat: a neonatal priming.

Rats were castrated on day 2 after birth, given one injection of testosterone pronate (TP: 2.5 mg) or oil just after operation and then received TP or oil when adult. 3H-Leucine was injected intravenously 2.5 hours before killing. The incorporation of labelled amino acid into protein was investigated in the thyroid glands. Males receiving TP both neonatally and when adult incorporate twice as much labelled leucine into protein as compared to their counterparts. Grain density was highest in the thyroid gland of early hormone treated rats when challanged with testosterone in adulthood. Thyroid gland priming by early hormone treatment has been discussed.

Adrenal Glands↗