PubMed Health⌕ Search

Biomedical subjects

M Novák

Publications and source records attributed to M Novák.

At least 181 records · Page 10Linked to original sources
PubMed1976

Electron microscopy of virus particles in ultrathin frozen sections.

A procedure with the use of polyethylene glycol (PEG) 600 to support biological materials for ultracryotomy was developed. Developing and mature virions were demonstrated by electron microscopy in negatively stained frozen sections of vaccinia virus-infected human diploid cells and of herpes simplex virus (HSV) type 1 infected primary rabbit kidney cells. Approximate localisation of the virus particles in the cells was possible.

Culture Techniques↗
PubMed1975

[Usability of methods for the determination of minute cardiac volume under clinical conditions].

Methods for determination of cardiac output are described and their evaluation and possibilities to use them in clinical practice mentioned. Out of the invasive methods apart from the classical Fick's method, the dye dilution method is of advantage during catheterization, being relatively simple and ready to be easily repeated if necessary directly at the bed side. The common disadvantage of all invasive methods is the disturbance of the patient's basal state. Noninvasive methods yield results suitable more or less for orientation only. From these techniques the indirect method of Fick using the rebreathing of CO2 is simple and can be applied at the bed side. So called physical methods are already obsolete at present.

Cardiac Catheterization↗
PubMed1975

Electron microscopy study of the development of neurovaccinia virus in rabbit blood leucocyte cultures.

In electron microscopy of rabbit blood leucocytes cultured after infection with a large dose of neurovaccinia virus, virus particles can be observed within 24 hours in the cytoplasm of the monocytoid cells (monocytes and macrophages) and occasionally in the polymorphonuclear cells, but never in the lymphocytes. The morphology of the virus particles does not differ from the description of these particles in other types of host cells. The virus replication cycle in the monocytoid cells is slow and the virus matures asynchronously. Virus replication causes infected cells to disintegrate.

Animals↗
PubMed1975

Effect of 60amino-6-deoxy-D-glucose hydrochloride on influenza infection in mice.

A prophylactic effect of 6-amino-6-deoxy-D-glucose hydrochloride (AG) in influenza-infected mice was demonstrated. A dose of 500 mg AG per kg body-weight given intranasally 2000 mouse intranasal LD50) protected 32-40 per cent of the animals from death. The virus titres in the lungs and serum were lower in treated mice than in untreated ones. After AG administration, low interferon titres were demonstrated in the lungs and serum. The group of infected mice, untreated with AG, was examined electron microscopically. Virus was demonstrated on the surface of granular pneumocytes and in the lumen of lung alveoli.

Administration, Intranasal↗
PubMed1975

Electron microscopic demonstration of Crimean hemorrhagic fever virus in CV-1 cells.

Crimean hemorrhagic fever (CHF) virus was detected by electron microscopy in ultrathin sections of CV-1 cells. The virus particles appeared spherical or oval and occurred both intra- and extracellularly. Based on their size and density of their contents, two types of virions, probably representing different phases of the reproduction cycle of CHF virus, could be distinguished.

Animals↗