[Problem of hemodynamics in programmed so-called physiological cardiac stimulation].
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Biomedical subjects
Publications and source records attributed to M Novák.
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Hybridomas secreting monoclonal antibodies with haemagglutination-inhibition (HI) activity to the Skalica strain of tick-borne encephalitis (TBE) complex were prepared by the fusion of P3-NS1-Ag4-1 myeloma cell line with spleen cells of BALB/c mice immunized with the purified Skalica strain. The highest titres of monoclonal antibodies obtained from the hybridomas S-9, S-15 and S-16 ranged from 512 to 10,240, respectively; the ascitic fluid contained as many as 4.6 mg/ml of monoclonal antibodies. Its analysis by Ouchterlony's double immunodiffusion, agarose electrophoresis, and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) revealed the presence of monoclonal antibodies with mu isotype of the heavy and kappa isotype of the light chain. The specificity of the monoclonal antibodies was proved using 11 different antigens from family Togaviridae in the HI test.
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Electron microscopy of 57 needle biopsies from livers of 55 patients with chronic hepatitis, mostly of type B, revealed, in addition to typical hepatitis B virus nucleocapsids in 34 cases, also atypical intranuclear inclusions in 9 biopsies. These inclusions consisted of electron-dense particles similar in size to hepatitis B virus nucleocapsids. The possibility of an involvement of a hepatitis non-A non-B virus is discussed.