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Biomedical subjects

M O Birger

Publications and source records attributed to M O Birger.

At least 19 recordsLinked to original sources
PubMed1992

[Intestinal microflora in young children with rotavirus infection].

A total of 270 children with rotavirus diarrhea were examined. The quantitative and qualitative composition of their intestinal microflora was studied. Most frequently microorganisms of the genus Enterobacter and most seldom, Serratia were isolated. A decrease in the amount of bifidobacteria, normal Escherichia coli and an increase in the amount of lactose-negative Escherichia were noted. In cases of pronounced dysbiosis in young children the clinical course of rotavirus infection is aggravated and the period of rotavirus excretion is prolonged.

Enterobacter↗
PubMed1991

[A method for assessing the microfloral status of the human intestines by the quantity of adhesion-active bacteria and by the type of adhesins].

The method for the evaluation of the state of intestinal microflora in the diagnosis of enteric diseases by the determination of the number of actively adhesive bacteria and the type of adhesins in the primary culture of human microflora has been developed. This method permits the simultaneous analysis of all colonies grown in a given Petri dish by the sign of their adhesion, which eliminates the possibility of mistakes due to the occasional choice of colonies, as it may happen in the agglutination test. The time necessary for the study of adhesive properties is reduced, as the result is evaluated as early as on day 2 after the inoculation of the seed material. It is also of importance that the method is simple in realization, needs no special equipment, preparation and staining of the material. To determine the type of adhesins, a series of identical inoculations may be obtained by replication with the use of red blood cells of different animals. The proposed method may be used for the confirmation of the etiological role of enterobacteria as evidenced by the presence of high percentage of their actively adhesive colonies.

Bacteria↗
PubMed1989

[Clinico-laboratory analysis of the diarrhea syndrome in young children excreting rotaviruses with feces].

A total of 404 children admitted to the infectious hospital of the non-intestinal type were examined with the aid of counter immunoelectroosmophoresis. Rotavirus antigen was detected in feces in 46.8% of 126 children admitted with a diagnosis of diarrhea, in 10.8% of 278 without diarrhea, and also in 32.9% of 149 children with diarrhea that occurred at the hospital. The clinical course of acute rotavirus gastroenteritis appeared to be materially influenced by acute respiratory viral infection, particularly influenza, and by certain types of opportunistic bacteria contained in high concentrations by the intestine.

Child, Preschool↗
PubMed1989

[Various biological properties of enterobacteria, isolated from patients with diarrhea].

381 enterobacterial strains isolated from patients with acute enteric diseases were studied. Of these, 279 strains, as well as 20 strains isolated from 50 healthy children, were studied for the presence of adhesins, hemolysins and catalase. The comparison of the hemagglutinating activity of enterobacteria isolated from sick and healthy children revealed no essential differences between them. 15.8% of enterobacterial strains isolated from sick children possessed hemolytic activity, while strains isolated from healthy children did not induce the hemolysis of erythrocytes. All enterobacterial strains isolated from patients with acute enteric diseases were multi-resistant to antibiotics. A multitude of different antibiograms was obtained, most of them occurring only once. In 1985 the number of multiresistant (i. e. resistant to 11 and more antibiotics) strains dropped from 61% to 26..9% in comparison with 1981.

Bacterial Adhesion↗
PubMed1988

[Etiological structure of acute intestinal infections caused by opportunistic bacteria in young children].

Altogether 587 children with diagnosed acute enteric infections were examined. In 26.4% of them pathogenic enterobacteria were detected, and in 59%, opportunistic enterobacteria. In 38.2% of children these bacteria were found to be the etiological factor of the disease. A reverse relationship between the contamination of feces with pathogenic enterobacteria and the age of children was established.

Acute Disease↗
PubMed1987

A study on the adhesive properties of Cor. diphtheriae and Cor. parvum in the direct haemagglutination reaction.

The adhesive properties of one Cor. parvum and two Cor. diphtheriae strains were investigated by means of the direct haemagglutination reaction (HAR). Using a specifically developed HAR procedure, it was proved beyond doubt that the studied Corynebacteria possess adhesive properties apparently associated with the presence of pili (fimbrias) on their surfaces. In addition, a correlation was found between the agglutinating activity of bacteria and the conditions of culture growth. Based on the reported results, it is suggested that there exists an intraspecies stability of adhesive properties and a possible link between corynebacterial adhesins and reticulostimulin.

Animals↗
PubMed1987

Using SIB and API kits to identify and biotype enterobacteria of different origin.

A collection of 26 Enterobacteriaceae reference strains provided by Reference Centres in Moscow (USSR) and Copenhagen (Denmark) as well as a collection of 660 freshly isolated cultures of Gram-negative bacteria of different origin were investigated using SIB indicator systems manufactured at the Gorky Institute of Epidemiology and Microbiology (USSR) and API-20E, Rapid-20E and API-10S kits (API, France) with the aim of species determination. In analyzing freshly isolated cultures, API-20E, API-10S and SIB-B kits proved to be of approximately equal efficiency, whereas the Rapid-20E system enabled species identification in no more than 78% of the tested cultures. In a model biotyping of 284 E. coli cultures of different origin, SIB-B and API-20E kits in combination with the Analytical Profile Index enabled sufficiently rapid and standard identification of Enterobacteriaceae biovars.

Bacteriological Techniques↗
PubMed1980

[Protective somatic antigen of Corynebacterium diphtheriae].

Deoxycholate C. diphtheriae extract was separated into fractions differing in the size of their molecules. Correlation between the molecular weight of each fraction, its specific protein content and its immunogenicity was observed. The high-molecular fraction (mol. wt. exceeding 300,000 daltons) was shown to be the protective somatic antigen of C. diphtheriae.

Animals↗
PubMed1977

[Determination of the intensity of antimicrobial immunity in diphtheria during an acute experiment].

The authors elaborated a method of determination of the antimicrobial immunity intensity in diphtheria in an acute experiment: guinea pigs were infected with a virulent culture of Corynebacterium diphtheriae grown on agar with iron in a concentration depressing the toxinogenesis. A temporary absence of the toxin formation permitted to evaluate the antibacterial immunity except the antitoxic one. Guinea pigs preimmunized with somatic Corynebacterium diphtheriae antigen or an immune antimicrobial serum constituted the experimental group. The antimicrobial protection level was assessed by the difference in the mortality rate of guinea pigs in the experimental and control groups.

Acute Disease↗