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M O Nielsen

Publications and source records attributed to M O Nielsen.

13 recordsLinked to original sources
PubMed1995

The local differential effect of prostacyclin, prostaglandin E2 and prostaglandin F2 alpha on mammary blood flow of lactating goats.

Mammary blood flow (MBF) and milk yield are closely related in dairy ruminants, but little is known about the regulation of MBF in vivo. The local effects on MBF of injections or continuous infusions into the mammary artery of prostaglandins (PG) or indomethacin (an inhibitor of prostaglandins) respectively, were investigated in surgically prepared conscious goats. Prostacyclin (PGI2) was found to be a potent stimulator of MBF which increased linearly over the dose range 50-1000 ng. PGE2 was almost as potent as PGI2 at low doses, but tachyphylaxis occurred at doses at and above 100 ng. The response to repeated injections of PGE2 quickly declined depending on the dose. PGF2 alpha had no effect on MBF. During infusion of indomethacin into the mammary artery MBF was reduced markedly, showing that endogenous mammary prostaglandins are involved in the regulation of vasodilatation. The results indicate that PGI2 (and to a lesser extent PGE2) has an important role in the local regulation of vascular tone in the mammary gland.

Animals↗
PubMed1994

[Alternatives to acute admissions to a city hospital. Is it possible to reduce the number of acute admissions?].

The purpose of the study was to evaluate the number of inappropriate admissions to a smaller city hospital and find possible alternatives. Physicians and surgeons from three units (abdominal surgery, internal medicine and orthopaedic surgery) together with general practitioners, doctors on homecall duty and health personnel from the region contributed to the study. In all 421 consecutive patients were included during a three-week period. The patients' median age was 60.5 years. It was found that at least 13.6% of all patients seemed to have been admitted for an inappropriate reason. According to the admitting doctor 3.4% of the patients were not ill at all. The investigation implies that one out of seven acute admissions could be replaced by alternatives such as immediate care in residential homes, more flexible contact to the outpatient's clinic, better access to geriatric evaluation and improved laboratory service. We conclude that acute admission to hospital can be replaced by other alternatives, thereby achieving greater efficiency and better economics.

Adult↗
PubMed1994

Changes in mammary uptake of free fatty acids, triglyceride, cholesterol and phospholipid in relation to milk synthesis during lactation in goats.

Uptake of free fatty acids (FFA), triglycerides (TG), cholesterol (CHOL) and phospholipids (PL) was measured in both mammary glands of dairy goats during lactation. Arterial concentrations of TG, CHOL and PL as well as arterio-venous difference (AV) and extraction rate (E) for TG were highest in goats with the highest dietary feed intake. AV were linearly related to arterial concentrations for the four lipid classes, and arterial concentrations of CHOL were linearly related to output of lactose, protein and fat in milk. Arterial supply, and not mammary synthetic activity, is the main determinant of mammary FFA, TG and CHOL uptake.

Animals↗
PubMed1994

Studies on responses to potassium, noradrenaline, serotonin, histamine and prostaglandin F2 alpha, of isolated pudendal arteries from non-lactating goats.

The effects of potassium (K+), noradrenaline (NA), serotonin (5-HR), histamine (Hi) and prostaglandin F2 alpha (PGF2 alpha) were studied on isolated pudendal arteries from non-lactating goats. K+, NA, 5-HT, Hi and PGF2 alpha had concentration-dependent contractile effects on the arteries. The developed tensions were, in order of potencies, 5-HT > NA > PGF2 alpha > Hi > K+. NA induced a significantly higher maximal contractile force than K+, 5-HT, PGF2 alpha and Hi. Pretreatment with cocaine was without significant effect on the contractile response to NA. The contractile response to Hi was totally eliminated by mepyramine. Ca2+ had a concentration-dependent contractile effect on arteries depolarized by 125 mM K+. The results indicate that NA, 5-HT and PGF2 alpha may play essential roles in the regulation of goat mammary blood flow. The response to K+ is highly dependent on extracellular Ca2+. The contractile response to histamine may be mediated via H1-receptors. The in vitro method used is well suited to study the vascular reactivity in different regions of the mammary vascular bed.

Animals↗
PubMed1993

Changes in mammary glucose and protein uptake in relation to milk synthesis during lactation in high- and low-yielding goats.

1. Glucose and protein uptake were measured in both mammary glands of two low- and two high-yielding dairy goats during lactation. 2. Low-yielding goats tended to have higher arterial glucose concentrations, but approximately 40% lower arterio-venous differences (AV) and extraction rates (E) for glucose than high-yielding goats. 3. AV and E for glucose (but not protein) were linearly related to yields of both lactose, milk protein and fat. 4. Mammary uptake of glucose is determined primarily by mammary glucose metabolism, not glucose supply; lower intracellular glucose concentration in mammary cells of genetically superior animals thus explains the more efficient mammary uptake.

Animals↗
PubMed1990

Changes in mammary blood flow during the lactation period in goats measured by the ultrasound Doppler principle.

1. Mammary blood flow was measured during the lactation period in two low- and two high-yielding dairy goats (peak milk yields approx. 1.5 and 3.6 kg/day respectively), using the ultrasound Doppler principle for determination of blood velocity in both milk veins (subcutaneous abdominal veins) of the animals, and ultrasound scanning for measurement of cross-sectional area of the veins. 2. Milk vein cross-sectional area ranged from 0.11 to 0.38 cm2 in the four goats, with a close to significant (P = 0.06) difference between the veins in the two sides of the animals. Cross-sectional area remained constant during the lactation period. Changes in mammary blood flow was therefore caused by changes in blood velocity. 3. Milk vein blood velocities ranged from 4.4 to 34.7 cm/sec independently of the time of the day, and were of the same magnitude in the two sides of the animals. Except in one goat (P = 0.1), blood velocity decreased significantly (P less than 0.01) with progressing lactation, during which period also milk yield was declining. 4. In the two low-yielding goats, a positive linear relationship (R2 = 0.20) was found between milk yield and milk vein blood velocity, whereas a diminishing exponential relationship (R2 = 0.97) was found in the two high-yielding goats. At a given milk vein blood velocity, high-yielding goats obtained a higher milk yield and also responded to changes in blood velocity (up to approx. 15 cm/sec) with greater increases in milk yield than low-yielding goats.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1990

Insulin-like growth factor I (somatomedin C) in goats during normal lactation and in response to somatotropin treatment.

1. Two experiments were performed. In Experiment 1, IGF-I concentrations in serum were followed in four lactating dairy goats during normal lactation and before, during and after 14 days of daily administration of 10 IU recombinant bovine somatotropin, starting 10 weeks post-partum. In Experiment 2, IGF-I was measured in single blood samples obtained from 40 dairy goats in the first part of lactation. 2. Measured IGF-I concentrations ranged from 4.4-9.0 nM. Concentrations varied during the day with coefficients of variation from 8.9 to 18.4% in the four goats in Experiment 1. There were no systematic trends in the diurnal variations (P greater than 0.75). 3. Concentrations of IGF-I remained fairly constant in all goats in Experiment 1 during the lactational period, and were not related to the changes in milk yield (P greater than 0.65). Similarly, no relationship was found in Experiment 2 between level of milk production and IGF-I concentration in serum. 4. Somatotropin treatment stimulated milk yields by 14 +/- 2.9% (P less than 0.05) and IGF-I concentration by 27.9 +/- 6.7% (P less than 0.05). 5. IGF-I is the most likely factor which increases mammary gland synthetic capacity in response to somatotropin treatment, and thus is responsible for the galactopoietic effect of this hormone being exerted. 6. However, serum IGF-I may primarily be involved in regulation of nutrient partitioning during normal lactation, but a paracrine synthesis with local effect on the mammary gland cannot be ruled out.

Animals↗
PubMed1989

The excretion of prostacyclin (PGI2) in milk and its possible role as a vasodilator in the mammary gland of goats.

1. Prostacyclin production in mammary gland of two lactating goats measured as the excretion in milk of 6-ketoprostaglandin F1 alpha (6-KPGF1 alpha) was followed for 16 days before, during and after exogenous administration of recombinant bovine growth hormone (GH). 2. 6-KPGF1 alpha was detected in all milk samples in concentrations ranging from 32-99 pg/ml milk independently of the time of sampling. 3. GH-treatment significantly increased milk yield, the concentration and excretion of 6-KPGF1 alpha in milk. 4. The concentration of milk 6-KPGF1 alpha was positively correlated with milk yield in the high (R2 = 0.35), but not in the low yielding goat (R2 = 0.003). 5. The possible role of prostacyclin as a local vasodilator in the mammary gland of goats is discussed.

6-Ketoprostaglandin F1 alpha↗
PubMed1989

Evaluation of mammary blood flow measurements in lactating goats using the ultrasound Doppler principle.

1. Non-invasive methods were developed for measuring mammary blood flow in lactating goats. 2. A Doppler principle ultrasound device was equipped with an external detector measuring maximal blood velocity (Vmax) and average blood velocity (Vav) was calculated as Vmax/2. Volume flow then depended on determination of the angle of insonation and the cross-sectional area of the milk vein (the caudal superficial epigastric or subcutaneous abdominal vein). 3. Blood velocities were measured on the milk vein of either side of the animal while clamping the pudendal veins manually. Blood velocities ranged from 7-34 cm/sec. 4. The milk vein diameter was measured by means of a slide gauge which, for clearly protruding veins, gave similar results to that measured by ultrasound scanning. In protruding veins the cross-section was circular. In non-protruding veins the cross-section was elliptical and the slide gauge significantly (P less than 0.01) overestimated the cross-sectional area. The milk vein diameter of either side measured in 10 lactating goats was 8.8 +/- 1.1 mm (means +/- SD). 5. Blood flow ranged from 90-675 ml/min in a dry and a high-yielding (3.4 l milk daily) goat, respectively. The reproducibility of the blood flow measurements was 12-16%. 6. It is concluded that the present method may be used for quantitative measurements of mammary blood flow in goats.

Animals↗