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Biomedical subjects

M O Raushenbakh

Publications and source records attributed to M O Raushenbakh.

At least 19 recordsLinked to original sources
PubMed1987

[Effect of the carcinogenic metabolites of aromatic amino acids on oxidative processes in lipids in vitro and in vivo].

A comparative study of the effect produced by endogenous carcinogens (p-hydroxyphenyllactic and 5-methoxyindole-3-acetic acids) and their non-cancerogenic analogues on lipid peroxidation in vitro and in vivo was performed. It has been found that cancerogenic tyrosine and serotonin metabolites, unlike their non-cancerogenic analogues, increase lipid peroxidation in vitro. In vivo, cancerogenic tyrosine metabolite--p-hydroxyphenyllactic acid--is capable both of increasing and decreasing antioxidative lipid activity in the animal liver.

Animals↗
PubMed1986

[Transplacental carcinogenic effect of the serotonin derivative 5-methoxyindoleacetic acid].

Transplacental administration of serotonin metabolite 5-methoxyindole-3-acetic acid possessing blastomogenic activity induced the development of malignant and benign neoplasms (lymphosarcomas, adenomas, hepatomas and other tumours) in 84% of C57BL/6 mouse progeny. The number of neoplasms was significantly higher, than in the control, they appeared earlier and were more malignant.

Animals↗
PubMed1986

[Effect of the carcinogenic tyrosine metabolite p-hydroxyphenyllactic acid on the ascorbic acid concentration in the organs and blood of mice].

The effect of cancerogenic tyrosine metabolite, p-hydroxyphenyllactic acid, on the concentration of ascorbic acid in the organs and blood of mice has been studied. p-Hydroxyphenyllactic acid was demonstrated to decrease considerably ascorbic acid concentration in the liver, adrenal glands and blood of mice. The above phenomenon and the previous data on tyrosine aminotransferase induction by p-hydroxyphenyllactic acid suggest the existence of two interdependent mechanisms of cancerogenic tyrosine metabolite (p-hydroxyphenyllactic acid) accumulation.

Animals↗
PubMed1985

[Blastomogenic activity of biogenic methoxyindoles].

A considerable blastogenic activity of biogenic methoxyindoles--melatonin, 5-methoxytryptamine (5-MOT) and their common metabolite--5-methoxyindolyl-3-acetic acid (5-MIAA) was established by prolonged s.c. injection in C57BL/6 mice. However, the blastogenic effect of 5-MOT decreased by 26% when its further metabolism to 5-MIAA was blocked for some time and the synthesis of its carcinogenic metabolite was inhibited. These results showed that the blastogenic effect of 5-MOT is not direct; and it is mediated by its transformation to 5-MIAA in the body.

5-Methoxytryptamine↗
PubMed1985

[Carcinogenic action of 5-methoxytryptamine related to its transformation into 5-methoxyindolyl-3-acetic acid].

A considerable blastomogenic effect of metabolite serotonin 5-methoxytryptamine (5-MOT) subcutaneously administered for a long time to C57BL/6 mice was established. This effect was decreased noticeably if the further metabolism of 5-MOT into 5-methoxyindolyl-3-acetic acid (5-MIAA) was blocked by pyrazidol. These results explain the fact that the blastomogenic effect of 5-MOT is not direct but is caused by the transformation of 5-MOT into its final carcinogenic metabolite 5-MIAA.

5-Methoxytryptamine↗
PubMed1984

[Morphological and cytochemical characteristics of spontaneous hemoblastoses in AKR mice].

Spontaneous hemoblastoses of AKR mice are widely used in experimental oncology and hematology. To study their morphological characteristics, 500 AKR mice were used. Of these, 400 animals were followed up throughout their lives. Ten animals were sacrificed monthly out of the group of 100 rats. All the animals which died or were sacrificed were autopsied and subjected to cytological and histological studies. Besides, 36 animals with hemoblastoses were examined cytochemically for the activity of acid and alkaline phosphatases, peroxidase and nonspecific esterase and glycogen. Analysis of the data obtained suggests that hemoblastoses of AKR mice are the generalized forms of lymphosarcoma, characterized by a significant degree of cytochemical and cytological heterogeneity.

Acid Phosphatase↗
PubMed1983

[Lectin-dependent cytotoxicity of human peripheral blood lymphocytes].

The evaluative technique of lymphocyte cytolytic activity in human peripheral blood has been designed. The target cells, lectin (Con A) concentration and incubation time for measuring cytolytic activity of lymphocytes pre-purified from adherent cells have been selected. The mean values of lectin-dependent cytotoxicity in peripheral blood of 50 healthy donors are presented.

Animals↗
PubMed1982

[Characteristics of the secretory apparatus of memory T-cells].

It has been shown that by day 7--8 of cultivation, large lymphocytes and lymphoblasts disappear, the DNA synthesis and cytolytic activity decrease in lymphocyte suspension enriched with a fraction of lymphoblasts obtained on the 5th day of mixed lymphocyte culture. The cytoplasm of medium-size and small lymphocytes adsorbed on the surface of target-cells manifests no signs of secretion: there are no tubular structures or rough reticulum, the Golgi complex is underdeveloped. It is suggested that there is a relationship between the secretory apparatus and cytolytic activity of T-killers.

Adsorption↗
PubMed1981

[Effect of L-ascorbic and D-isoascorbic acids on induced formation of tyrosine aminotransferase in rat liver].

Administration of ascorbic acid at moderate doses/0.05-0.1 g per kg of body mass/was shown to induce tyrosine transaminase in liver tissue of intact rats. The enzyme induction depended on the state of adrenal glands and was inhibited by actinomycin D. As distinct from the native form of L-ascorbic acid moderate doses of D-isoascorbic acid did not induce the enzyme in rat liver tissue; these data suggest the relative biological inactivity of D-isoascorbic acid.

Adrenal Glands↗
PubMed1978

[Ultrastructure of the conjugates of cytolytic T-lymphocytes and target cells].

Conjugates of target cells and of cytological T-lymphocytes obtained on the 11th day after alloimmunization were investigated. The conjugates formed small and medium lymphocytes; mature secretory granules, crystal-like structures and lipids were revealed in their cytoplasm. The lymphocyte is spherical, the area of contact with the target cell does not exceed 5 to 15%. Cytolysis of target cells is observed after 30 to 60 minutes of incubation. The lymphocyte becomes flattened, its nucleus acquires an oval form, and the area of contact with the target cell increases considerably. At the same time hypertrophy and change of orientation of Golgi's complex to the area of contact with the target cell, coalescence of the secretory granules with lipids and crystal-like structures, the appearance of immature secretory granules and vacuolar degeneration of mitochondria are demonstrated. The lymphocyte membrane becomes "desquamated"; structures connected with it, named "membranosomes" are described. It is suggested that the secretory processes in the cytoplasm of cytolytic T-lymphocytes are activated in their interaction with target cells.

Animals↗
PubMed1977

[Effect of avitaminosis B6 in mice on the function of the T-lymphocytes in vitro].

The influence of different degrees of avitaminosis B6 in mice on the cytolytic activity of T-lymphocytes measured by the amount of Na2Cr51O4 released from the lysed target cells was studied on a model of primary immune response in a mixed lymphocyte culture in vitro. Keeping of the animals for 3 weeks on pyridoxine-free diet failed to influence the capacity of lymphocytes to proliferate in vitro and their cytolytic activity. In animals on pyridoxine-free diet for 45 days the amount of pyridoxal 5(1)-phosphate in the spleen decreased by 55% in comparison with control. Lymphocytes obtained from these animals and cultivated in vitro had a markedly decreased capacity to 3H-thymidine incorporation into DNA in response to alloantigen. The cytolytic activity of these lymphocytes also diminished. The capacity of various pyridoxine forms to restore T-lymphocyte functions disturbed by avitaminosis B6 was studied.

Animals↗