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M Ollero

Publications and source records attributed to M Ollero.

36 records · Page 2Linked to original sources
PubMed1991

[Spread pattern of HIV-2 in patients at risk. Evaluation of serologic markers].

The aim of this paper is to ascertain the spread pattern of HIV-2 in patients at risk in an area of frequent contact with sub-Saharan Africa and evaluate the seric marker of HIV-2 infection. 606 serum samples from 524 patients were analysed. 445 patients were intravenous drug users (IDU), 17 used inhaled drugs, 26 had HIV-2 (+)partner, and others 36 high-risk practice. The ser were analysed for HIV-1 and HIV-2, using ELISA, Western blot (LAVBLOT 1, LAVBLOT 2) and specific synthetic peptides such as gp 41 and gp 36 (PEPTI-LAV 1-2). 245 out of 524 (47%) patients were HIV-1 (+). 65 out of 524 (12.4%) were Elavia-2 and HIV-1 (+). All samples tests using Western blot 1, confirmed the results of the ELISA (2 protein of the membrane). The Western blot of HIV-2 Elavia-2 (+) in 2/53 showed two proteins of the membrane and 26/53 one protein of the membrane and several of the core. The PEPTIL-LAV 1-2 was HIV-1 positive in 77/77 HIV-1 serum samples and in 10 HIV-2 positive, however, in all cases the HIV-2 reactivity was lower than HIV-1, allowing the orientation of the diagnosis towards the HIV-1 positive serum. In spite of the frequent contact with Africa, the spread of HIV-2 in the south of Spain is still quite insignificant. The Western blot of HIV-2 gives a high proportion of double reactivity, including those cases with restrictive criteria to be positive. PEPTI-LAV 1-2 has proved most useful in the diagnosis orientation in the cases of crossed reactivity between HIV-1 and HIV-2.

Adult↗
PubMed1991

[HTLV-I infection in sailors who travel to sub-Saharan West Africa].

In order to assess if HTLV-1 retrovirus had penetrated in a group of sailors that often travel to endemic areas for HTLV-1 infection (West coast of sub-Saharan Africa) we have performed the following study. A total of 499 sera samples from sailors traveling to Africa during the last five years were studied. The presence of HTLV-1 antibodies was determined using an enzyme-linked immunosorbent assay (ELISA, Dupont HTLV-1). Positive ELISA test results were further confirmed using a Western blot technique. In all patients the presence of HIV-1 and HIV-2 antibodies was also tested using ELISA and Western blot techniques. Using ELISA we find 7 positive sera to HTLV-1 (1.4%) but only one sera sample (0.2%) was also positive by Western blot analysis. We conclude that HTLV-1 infection is entering a population group traveling to endemic areas. The possibility of heterosexual transmission of the virus in our environment has to be taken into account.

Adult↗
PubMed1990

[HTLV-I infection in a high-risk group].

The aim of this study is to detect the presence of HTLV-1 in a high-risk population in west Andalusia. We studied 267 samples of serum from 255 patients: 179 of these patients being intravenous drug-users, 14 had ADVP sexual partners, 16 were inhalation drug-users, 4 were hemophiliacs, 9 had other high-risk habits and 25 hematological patients afflicted with leukemia or lymphoma. All of them were tested for antibodies against HTLV-1 by means of an in vitro qualitative ELISA technique (ELISA Du Pont HTLV-1). The positive results were confirmed by the Western blot technique. Additionally, the p24 antigen and the antibodies against VIH-1 and VIH-2 (ENV/CORE) were analysed, except in the 25 hematological patients. We found 20 serum samples positive to HTLV-1 by ELISA (7.4%), but only 1 (0.3%) was confirmed by the Western blot technique. The prevalence of VIH-1 was 46%; 9% had p24 VIH antigen and 26% had false positive ELISA to VIH-2. We found a statistically significant relationship (p = 0.0005) between positive ELISA to HTLV-1 and antibodies against VIH. We conclude that HTLV-1 has penetrated into the high-risk population of west Andalusia , although not yet to a great degree, and point out the need for seric epidemiological surveillance to prevent the spread of the retrovirus in these groups.

False Positive Reactions↗
PubMed1985

Antibodies to human T-cell leukemia virus type III in hemophiliacs from Spain.

We tested serum samples from 50 hemophiliacs from Sevilla, Spain, for antibody to HTLV-III by indirect membrane immunofluorescence (IMI) and radioimmunoprecipitation with SDS polyacrylamide gel electrophoresis (RIP-SDS/PAGE). All had received commercial clotting factors from the United States with the exception of one hemophiliac who had never been transfused. Thirty-four (68%) reacted with HTLV-III-infected cells (H9/HTLV-III) by both methods, but not with the uninfected line (H9). Of 41 hemophilia-A patients tested, 28 (68%) were positive, and of nine hemophilia-B patients, six (66%) were positive. The nontransfused hemophilia-B patient was negative for antibody to HTLV-III by both methods. One patient with clinical AIDS tested positive as did six of seven with chronic unexplained lymphadenopathy. The eight individuals with AIDS or lymphadenopathy all had hemophilia A. We conclude that exposure to HTLV-III is widespread among asymptomatic hemophiliacs in Spain.

Adolescent↗
PubMedDate not supplied

Antibodies to the AIDS-associated retrovirus in hemophiliacs and other individuals from southern Spain: lack of correlation with lymphocytes sub-populations.

Exposure to the AIDS-associated retrovirus (ARV) has been observed in 54 hemophiliacs in Southern Spain who have received commercial coagulation factors manufactured in the United States. Contact with ARV could be detected in some sera collected in 1982. Frequency among asymptomatic hemophiliacs was 44%, in individuals with lymphadenopathy syndrome 71%, and 2 AIDS cases were seropositive. Evidence for exposure to ARV did not correlate with changes in the lymphocyte populations or in the clinical condition of the patients. Only the amount of factor received distinguished the seropositive and the seronegative groups of hemophiliacs. Exposure to ARV was also detected in sera from homosexual men and I.V. drug addicts. The results indicate that members of AIDS risk groups in Spain have had contact with the AIDS retrovirus.

Antibodies, Viral↗
PubMedDate not supplied

[Sphenoid metastasis mimicking a meningioma as the initial feature of adenocarcinoma of the prostate].

INTRODUCTION: Presentation of adenocarcinoma of the prostate as an intracranial metastasis is uncommon. This metastasis tends to be seen in the sphenoid bone, and in this case it is difficult to differentiate radiologically from a meningioma. Because this cancer is treatable, the differential diagnosis must be resolved as soon as possible. CLINICAL CASE: A 72 year old man presented with a rapidly progressive left parasellar syndrome. On neuroimaging there was a tumour at the level of the left lesser wing of the sphenoid, which was isodense and iso-intense and also showed homogeneous uptake of contrast material. On angiography the circulation was pathological with homogeneous delayed filling. The initial diagnosis was meningioma of the lesser wing of the sphenoid. Months later the patient complained of bone and respiratory problems. At this time plain chest X-ray was compatible with carcinomatous lymphangitis. Therefore, tumour markers were studied and for the first time the specific prostatic antigen was investigated and found to be raised. Although there were no symptoms of prostatism, per rectum there was a malignant prostatic mass. In spite of complete hormone block, his illness followed an unfavorable course. At necropsy there was adenocarcinoma of the papillary prostate and a metastasis in the left lesser wing of the sphenoid. CONCLUSIONS: In elderly men, detection of a sphenoid tumour, which radiologically may appear to be a meningioma and although prostatism has not been diagnosed, the possibility of prostatic metastasis should be considered. Per rectum examination and specific prostatic antigen determination should be done in these patients.

Adenocarcinoma↗
PubMedDate not supplied

Separation of ram spermatozoa bearing X and Y chromosome by centrifugal countercurrent distribution in an aqueous two-phase system.

The availability of reliable quantification techniques of X and Y chromosome-bearing spermatozoa in a given insemination dose would allow further approaches to their separation, which is a topic of unquestionable interest in animal production. The aim of the current work was the development of a combined approach of polymerase chain reaction and countercurrent distribution to address both objectives. First, using Sac/polymorphisms for ZFX/ZFYloci in sheep deoxyribonucleic acid, a linear correlation has been established between the densitometric quantification of the restricted fragment length polymorphisms corresponding to the amplified loci ZFX/ZFY by polymerase chain reaction and the theoretical proportions of X and Y chromosomes in standard solutions. The method, subsequently applied to semen samples, estimated an equal proportion of spermatozoa bearing each chromosome. Second, by using centrifugal countercurrent distribution in a sensitive-charge aqueous two-phase system, we achieved the separation of a sperm population enriched in Y chromosome-bearing ram spermatozoa (75%) with a high viability rate (57%).

Animals↗
PubMedDate not supplied

Improvement of cryopreserved ram sperm heterogeneity and viability by addition of seminal plasma.

The effect of seminal plasma, as well as some seminal plasma fractions, on the heterogeneity and viability of frozen-thawed ram spermatozoa was studied. Fresh and frozen sperm samples were simultaneously assessed by a routine semen-quality assay and centrifugal countercurrent distribution (CCCD) in an aqueous two-phase system analysis. All samples used for this study were obtained from Salz rams by an artificial vagina and frozen according to the Fiser method. Sperm samples, consisting of either raw or washed semen, were frozen in the presence of either whole ram seminal plasma (RSP) or > 10 kDa seminal plasma fraction. Two control samples were considered: one diluted in Fiser's extender and an undiluted sample. Cell-membrane integrity (viability), hypoosmotic swelling (HOS) test, and sperm motility were assessed in all cases after collection, before freezing (at 5 degrees C), after thawing, and after removing the diluent before CCCD analysis. Our results show that sperm cell partition behavior was dependent on the sperm treatment and that the freezing-thawing process accounted for a clear loss of sperm heterogeneity and membrane integrity. These losses were less apparent when seminal plasma had been removed from semen samples before freezing. In addition, washed semen frozen in the presence of either > 10 kDa seminal plasma fraction or whole re-added seminal plasma maintained a higher rate of sperm heterogeneity and viability.

Animals↗