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M Onodera

Publications and source records attributed to M Onodera.

At least 55 records · Page 3Linked to original sources
PubMed1995

Immunological detection and characterization of an estrus-associated antigen in the goat oviduct.

The present study was designed to investigate the possibility that the goat oviduct produces specific substance(s) similar to the oviductal glycoprotein (BOGP) of the bovine oviduct. Oviductal flushings obtained from goats at the follicular and luteal phases of the estrous cycle were examined by immunoblotting for the presence of material that cross-reacted with monoclonal antibodies (MAbs) specific for BOGP. The MAbs immunoreacted with a broad band with a molecular mass of protein of about 97 kDa in the flushings of goat oviducts after fractionation of proteins by electrophoresis under reducing conditions. The antigen was present in flushings obtained from the ampullar segments of oviducts from goats at the follicular stage, but this antigen could hardly be detected in the flushings from the ampulla at the luteal phase and or the flushings from the isthmus at either the follicular or the luteal phase. This antigen was not detected in uterine flushings, follicular fluid, or serum. An immunohistochemical study demonstrated that the MAbs reacted specifically with the epithelial cells of the goat oviduct. Intense labeling was observed in the ampullar and fimbrial epithelia of goat oviducts at the follicular phase, but the immunohistochemical reaction was very weak at the luteal phase. In the isthmus, the reaction was faint during both the follicular and the luteal phase. No specific immunohistochemical reactivity with the MAbs was observed with other tissues from the reproductive tract or with nonreproductive tissues. Immunofluorescent staining showed that the antigen was associated with the zona pellucida of goat ovarian eggs that had been incubated with ampullar flushings from goats at the follicular stage.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1995

Pancreatic involvement in patients with Sjögren's syndrome and primary biliary cirrhosis.

Serum pancreatic enzyme activities, exocrine pancreatic function, and pancreatic ductal morphology were evaluated in patients with one or both of Sjögren's syndrome and primary biliary cirrhosis. Ten of 20 patients with Sjögren's syndrome (50%), 6 of 17 patients with primary biliary cirrhosis (35%), and 4 of 11 patients with both diseases (36%) had an elevated level of at least one pancreatic enzyme, including elastase-1, lipase, and trypsin. Diminished excretion of N-benzoyl-L-tyrosyl-para-aminobenzoic acid was observed in 3 of 17 patients with Sjögren's syndrome (18%), 4 of 16 with primary biliary cirrhosis (25%), and none of 7 with both diseases. Endoscopic retrograde pancreatograms demonstrated an abnormal pancreatic ductal configuration in 3 of 11 patients with Sjögren's syndrome (27%), 2 of 9 with primary biliary cirrhosis (22%), and 3 of 4 with both diseases (75%). Only minimal changes in branches of the pancreatic duct were observed in the pancreatogram. Finally, 9-30% of patients with Sjögren's syndrome and/or primary biliary cirrhosis had a mild and intermittent abdominal pain. These findings support the concept of a disease complex, "autoimmune exocrinopathy," in patients with Sjögren's syndrome, primary biliary cirrhosis, and chronic pancreatitis.

4-Aminobenzoic Acid↗
PubMed1994

Identification of autoantibodies to a pancreatic antigen in patients with idiopathic chronic pancreatitis and Sjögren's syndrome.

A serum autoantibody to a pancreatic antigen was identified in patients with idiopathic chronic pancreatitis and Sjögren's syndrome by radioimmunoassay and Western immunoblotting. Antigen from porcine and human pancreas extracts was partially purified using a monoclonal antibody, SP3-1, which recognizes the antigen in duct cells of various exocrine organs. Solid phase radioimmunoassay of the pancreatic antigen showed a positive result in 6 of 20 patients with idiopathic chronic pancreatitis (30%), 3 of 11 patients with Sjögren's syndrome (27%), and 1 of 15 patients with alcoholic chronic pancreatitis (7%). Among seven patients with stone-related chronic pancreatitis, six patients with autoimmune thyroiditis, and 14 normal controls, none showed evidence of autoantibodies to the pancreatic antigen. Western immunoblotting showed that serum antibody commonly reacted with a 60-kD molecule of either porcine or human pancreatic antigen, with which SP3-1 also reacted. These results show the existence of the autoantibodies to pancreas, especially to an antigen expressed in ductal cells of exocrine glands, in idiopathic chronic pancreatitis and Sjögren's syndrome, and suggest the possibility of an autoimmune mechanism in the pathogenesis of idiopathic chronic pancreatitis.

Animals↗
PubMed1994

Immune complex specific for the pancreatic duct antigen in patients with idiopathic chronic pancreatitis and Sjögren syndrome.

Circulating immune complexes (ICs) containing the pancreatic antigen against SP3-1 monoclonal antibody were measured in patients with idiopathic chronic pancreatitis (ICP) and Sjögren syndrome (SjS) by Raji cell and solid-phase radioimmuno-assays (RIA). The mean serum levels of ICs measured by solid-phase RIA were significantly higher in patients with ICP (n = 23) and SjS (n = 21) than control (n = 15, p < 0.05, p < 0.02, respectively). ICs were positive in 10 patients with ICP (43%) and 12 SjS patients (57%). Raji cell assay also revealed a significantly higher serum ICs levels in patients with ICP (n = 17) and SjS (n = 12) compared with those of control (n = 7, p < 0.025, p < 0.005, respectively). Seven patients with ICP (41%) and 8 SjS patients (67%) had positive ICs. This was in contrast to the normal level of ICs in patients with alcoholic chronic pancreatitis, primary biliary cirrhosis, and chronic thyroiditis. Our analysis demonstrated a significant and positive relationship between RIA and Raji cell assay (r = 0.70, p < 0.05). Our results suggest that ICs specific for SP3-1 may play a role in the pathophysiology of ICP and SjS.

Alcoholism↗
PubMed1994

Multidrug resistance gene and P-glycoprotein expression in anaplastic carcinoma of the thyroid.

The resistance of malignant tumors to chemotherapy with anticancer drugs has been considered to be due partly to overexpression of the multidrug resistance gene (mdr1) and its gene product, P-glycoprotein (P-GP), which acts as a drug efflux pump for several chemotherapeutic agents. In order to elucidate the mechanism of anticancer drug resistance in anaplastic thyroid carcinoma with very poor prognosis, we examined the expression of mdr1 mRNA and P-GP, and analyzed their relationships to chemotherapy response. Twenty surgical samples from 16 patients with anaplastic thyroid carcinoma were used. The mdr1 mRNA expression was examined by reverse transcription and polymerase chain reaction, and P-GP expression was evaluated by an immunohistochemical method using JSB-1 monoclonal antibody. Of the 20 clinical samples, expression of mdr1 mRNA and P-GP was observed in three and four samples, respectively. Three of the patients from whom the samples were obtained had been given anticancer drugs before biopsy. Of 12 patients who received chemotherapy for clinically evaluable diseases, 2 responded well, but 10 showed no response. All except one patient died of cancer progression. There was no relationship between the response to chemotherapy and the expression of mdr1 and P-GP. The expression of mdr1 mRNA and/or P-GP was observed in 5 of 16 patients with anaplastic thyroid carcinoma. However, the appearance of anticancer drug resistance in anaplastic thyroid carcinoma may not be explained solely by the expression of mdr1 and P-GP.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed1994

[An overexpression of retinoic acid receptor alpha blocks myeloid cell differentiation at the promyelocyte stage].

Retinoic acid (RA), a vitamin A derivative, exerts a wide range of biological effects related to cell proliferation and differentiation. The pleiotropic effects of RA are thought to be mediated through specific nuclear RA receptors (RARs). RARs are members of the steroid/thyroid hormone receptor superfamily and exhibit a molecular structure that possess discrete DNA-binding and RA (ligand)-binding domains. In hematopoietic system, RA and RARs, predominantly RAR alpha may play key roles for the proliferation and differentiation of hematopoietic progenitors. However, it is currently unknown how RA and RARs are involved in regulating normal hematopoietic differentiation. To make clear the roles of RA and RAR alpha in the normal hematopoiesis, I have introduced the construct of human RAR alpha (hRAR alpha) into murine bone marrow cells with retroviral vector, and selected infected cells with drug resistant marker (Neo(r)) cultured on the stroma cell line (PA6-neo), and analyzed the behavior of infected cells. All of procedure were done in vitro. Most cells infected with hRAR alpha exhibited promyelocytic morphology and were thought to be blocked at the promyelocytic stage in their myeloid differentiation. Furthermore, these immature cells differentiated terminally into mature granulocytes by adding with RA (10(-6) M). RAR alpha infected cells were also able to differentiate into mature macrophages in the both of long term culture and IL3 colony. These observations suggest that an overexpression of RAR alpha alone is effective to suppress myeloid cell differentiation and RAR alpha plays a crucial role in the terminal differentiation of myeloid precursors. The system described here may serve as a model for studying the the essential genes for differentiation of normal bone marrow cells.

Animals↗
PubMed1993

Immunocytochemical localization of oviduct-specific glycoproteins in the oviductal epithelium from cows at follicular and luteal phases.

The immunocytochemical localization of bovine oviduct-specific glycoproteins was investigated by light and electron microscopy. Using monoclonal antibodies (MAbs) specific for bovine oviductal glycoproteins, 3 regions (fimbriae, ampulla, and isthmus) of the epithelium in the bovine oviduct we studied during the follicular and luteal phases. The MAbs reacted specifically with the oviductal epithelial cells. Intense labeling was observed in the ampullar and fimbrial epithelia of cows at the follicular phase, but the reactions were weaker at the luteal phase. In the isthmus, the immunohistochemical reaction was faint during both follicular and luteal phases. At the ultrastructural level, the MAbs bound selectively to putative secretory granules of nonciliated cells in the ampulla and fimbriae, but not in the isthmus. These results suggest that there are cyclic changes and regional differences in the production of glycoproteins in the bovine oviduct.

Animals↗
PubMed1993

A Japanese family of X-linked auto-immune enteropathy with haemolytic anaemia and polyendocrinopathy.

Three cases of X-linked auto-immune enteropathy with haemolytic anaemia and polyendocrinopathy are described from one related Japanese kindred. Two boys had died due to severe diarrhoea accompanied by total or subtotal intestinal villous atrophy. In contrast, although one patient showed the same symptoms and had circulating IgG antibodies against enterocytes, his condition improved dramatically and he developed well following the use of cyclosporin A (CSA). CSA may be beneficial in patients with this rare disorder. Auto-immune enteropathy should be considered as a cause of protracted diarrhoea with unknown aetiology.

Anemia, Hemolytic↗
PubMed1993

Scanning electron microscopy of goat oviductal epithelial cells at the follicular and luteal phases of the oestrus cycle.

The luminal surfaces of epithelial cells in various regions of the oviducts of the goats at the follicular and luteal phases of the oestrous cycle were examined by scanning electron microscopy. Marked cyclic changes were observed on the surface of the epithelium in the fimbriae, ampulla and ampullar-isthmic junction, but few changes were found in the isthmus or uterotubal junction. The epithelium of the fimbriae, ampulla, and ampullar-isthmic junction of oviducts in the follicular phase was extensively ciliated and most of the cilia extended above the apical processes of the nonciliated cells. In the luteal phase, many ciliated cells were hidden by the bulbous processes of the nonciliated cells. In the isthmus and at the uterotubal junction, the apical surfaces of the nonciliated cells were flat or gently rounded at both phases of the oestrous cycle. The results demonstrate that regional variations are associated with the cyclic changes in the epithelial cells of the goat oviduct.

Animals↗
PubMed1993

Reduced nm 23-H1 messenger RNA expression in metastatic lymph nodes from patients with papillary carcinoma of the thyroid.

The nm 23 gene product, which possesses nucleoside diphosphate kinase activity, is a possible mediator of cancer cell invasion and metastasis. It has been divided into two distinct gene products, nm23-H1 and nm23-H2. We have developed a method for detecting nm 23-H1 and nm 23-H2 RNA using the polymerase chain reaction, based on the amplification of complementary DNA copies of nm 23-H1 and nm 23-H2 RNA. Using this method, the nm 23-H1 and nm 23-H2 messenger (m)RNA levels in 35 thyroid papillary carcinomas, 11 metastatic lymph nodes from patients with thyroid papillary carcinomas, five thyroid follicular adenomas, and three normal thyroid tissue samples were studied. Both nm 23-H1 and nm 23-H2 mRNA were expressed ubiquitously in normal and malignant thyroid tissues. However, in metastatic lymph nodes no (3 of 11) or weak expression (8 of 11) of nm 23-H1 mRNA was observed, the extent of which was inversely proportional to the degree of cancer cell occupancy, whereas nm 23-H2 mRNA was expressed and the levels were similar to those in other tissue tested. These results show that nm 23-H1 only may play a role in cancer cell invasion and metastasis although the exact mechanisms involved have yet to be elucidated.

Adenoma↗
PubMed1992

Metabolic fate of nicotinamide in LEC rats.

Metabolic fates of nicotinamide in Wistar and LEC rats were compared. In Wistar rats (normal rats) nicotinamide was generally catabolized to N1-methyl-4-pyridone-3-carboxamide (4-Py) through N1-methylnicotinamide (MNA). But in LEC rats nicotinamide catabolism mainly stopped at MNA. Therefore, the excretion ratio of (N1-methyl-2-pyridone-5-carboxamide + 4-Py)/MNA, which is an index of amino acid adequacy, was much lower in the LEC rats than in the Wistar rats. This phenomenon was attributed to the fact that the 4-Py-forming MNA oxidase activity was much lower in the LEC rats than in the Wistar rats. The NAD contents in liver and blood were also lower in LEC rats than in Wistar rats. These results suggest that the ability to synthesize proteins such as 4-Py-forming MNA oxidase and dehydrogenases may be impaired in LEC rats.

Animals↗
PubMed1991

High-performance liquid chromatographic determination of 3-hydroxykynurenine with fluorimetric detection; comparison of preovulatory phase and postovulatory phase urinary excretion.

A high-performance liquid chromatographic assay for 3-hydroxykynurenine in human urine is described. A fluorescent derivative of 3-hydroxykynurenine was prepared, based on the reaction of the compound with p-toluenesulphonyl chloride in a basic medium. The analytical method for the measurement of the fluorescent compound employed a Tosoh ODS 80 column eluted with 10 mM potassium dihydrogenphosphate (pH 4.5) and acetonitrile (3:2, v/v) and detection at an excitation wavelength of 375 nm (10 nm bandpass) and an emission wavelength of 455 nm (10 nm bandpass). The column temperature was maintained at 25 degrees C. The detection limit was 3 pmol (673 pg) at a signal-to-noise ratio of 5:1. The fluorescent derivative of 3-hydroxykynurenine was eluted at ca. 12.5 min. The technique was applied to the analysis of human urine. The total analysis time was ca. 15 min.

Adult↗
PubMed1991

Growth requirements and long-term subcultivation of bovine granulosa cells cultured in a serum-free medium.

We have developed an improved serum-free medium to optimize the cell growth of bovine granulosa cells. The cells on collagen-coated culture plates proliferated extensively in a nutrient medium supplemented with insulin, heparin binding growth factor-2 (HBGF-2), lipoprotein, and bovine serum albumin (BSA). The cell doubling time at logarithmic phase and final cell density at confluent cultures were equal to those of cultures grown in the presence of medium supplemented with optimal concentration (10%) of fetal bovine serum (FBS). Whereas HBGF-2 or insulin alone had a small mitogenic effect of granulosa cells, lipoprotein or BSA did not. When lipoprotein, BSA, or insulin was added together with HBGF-2, synergistic cell proliferation was observed in all combinations. Insulin or lipoprotein had an additive mitogenic stimulation of these cells in the presence of BSA. After granulosa cells were subcultivated in a serum-containing medium until three generations [8.5 cumulative population doubling level (CPDL)], subsequent subcultivation of the cells in a complete serum-free medium could be achieved up to six generations (14.4 CPDL). These results demonstrate that this serum-free medium can support the optimal cell growth and long-term subcultivation of bovine granulosa cells.

Animals↗
PubMed1991

Observation of spinal canal and cisternae with the newly developed small-diameter, flexible fiberscopes.

Small-diameter (0.5-, 0.9-, and 1.4-mm) flexible fiberscopes were developed for visual diagnosis of spinal canal diseases. The fiberscopes were introduced via a Tuohy needle into the subarachnoid and epidural spaces of ten patients with various pain syndromes. Clear visualization of the subarachnoid space was achieved using the fiberscopes. The epidural space could be visualized only during withdrawal of the fiberscope. In five cases, the fiberscope could be advanced up to the level of the cisternae without causing the patient any discomfort. A slight headache and transient fever were noted after the examination in five and two cases, respectively, but no other complications occurred. Interestingly, preexisting pain diminished (two cases) or disappeared (one case) after the myeloscopy in three of five cases in which the myeloscopy revealed aseptic adhesive arachnoiditis. Further studies should be carried out to evaluate the usefulness of this technique.

Adult↗
PubMed1991

High-performance liquid chromatographic measurement of tryptophan in blood, tissues, urine, and foodstuffs with electrochemical and fluorometric detections.

A rapid and convenient measurement of tryptophan in whole blood, serum, liver, brain, urine, and alkaline hydrolysates of proteins and foodstuff was done by high-performance liquid chromatography. The sample preparation was simply homogenized or mixed in a 5% trichloroacetic acid solution and a sample of the supernatant was injected onto a column after filtration with a 0.45-micron filter. The method used a Chemcosorb 5-ODS-H column (particle size, 5 microns, 150 x 4.6 mm i.d.) eluted with 20 mM potassium dihydrogen phosphate (pH adjusted to 3.7 by the addition of phosphoric acid) containing 1 g/l of sodium heptane sulfonate and 3 mg/l EDTA.2Na-acetonitrile (93:7, v/v) at a flow rate of 1.5 ml/min. The tryptophan contents in whole blood, serum, liver, and brain were electrochemically estimated at +1000 mV vs. Ag/AgCl, the detection limit being 0.2 pmol (40.84 pg) at a signal-to-noise ratio of 5:1. The tryptophan contents in urine, proteins, and foodstuff were fluorometrically estimated with an excitation wavelength of 280 nm and with an emission wavelength of 340 nm, the detection limit being 20 pmol (4.08 ng) at a signal-to-noise ratio of 5:1. Tryptophan was eluted at about 10.5 min. The total analysis time was about 12 min.

Animals↗
PubMed1990

[Anesthesia for pectus excavatum].

The incidence of arrhythmia, postoperative complication and pulmonary oxygenation (PaO2) were studied in 48 patients with pectus excavatum scheduled for the Ravitch operation under halothane-nitrous oxide-oxygen (GOF) and enflurane-nitrous oxide-oxygen (GOE) anesthesia. Preoperative abnormalities of ECG were observed in 36 of 18 cases. Main abnormalities were incomplete right bundle branch block, left atrium enlargement, and sinus arrhythmia. Ventricular arrhythmia was observed in 4 of 12 cases during GOF anesthesia, whereas no arrhythmia was observed during GOE anesthesia. In postoperative chest X-ray, pulmonary atelectasis (60%), pleural effusion (48%), and pneumothorax (8%) were observed. The results suggest that GOE is more advantageous for pectus excavatum operation than GOF. Postoperative pulmonary surveillance is important for pectus excavatum operation.

Adolescent↗