Biomedical subjects

M Onorato

Publications and source records attributed to M Onorato.

At least 19 recordsLinked to original sources

PubMed2006

Modulational instability in crossing sea states: a possible mechanism for the formation of freak waves.

Here we consider a simple weakly nonlinear model that describes the interaction of two-wave systems in deep water with two different directions of propagation. Under the hypothesis that both sea systems are narrow banded, we derive from the Zakharov equation two coupled nonlinear Schrödinger equations. Given a single unstable plane wave, here we show that the introduction of a second plane wave, propagating in a different direction, can result in an increase of the instability growth rates and enlargement of the instability region. We discuss these results in the context of the formation of rogue waves.

Journal Article↗

PubMed2004

Observation of strongly non-Gaussian statistics for random sea surface gravity waves in wave flume experiments.

We study random surface gravity wave fields and address the formation of large-amplitude waves in a laboratory environment. Experiments are performed in one of the largest wave tank facilities in the world. We present experimental evidence that the tail of the probability density function for wave height strongly depends on the Benjamin-Feir index (BFI)-i.e., the ratio between wave steepness and spectral bandwidth. While for a small BFI the probability density functions obtained experimentally are consistent with the Rayleigh distribution, for a large BFI the Rayleigh distribution clearly underestimates the probability of large events. These results confirm experimentally the fact that large-amplitude waves in random spectra may result from the modulational instability.

Journal Article↗

PubMed2002

Freely decaying weak turbulence for sea surface gravity waves.

We study the long-time evolution of deep-water ocean surface waves in order to better understand the behavior of the nonlinear interaction processes that need to be accurately predicted in numerical models of wind-generated ocean surface waves. Of particular interest are those nonlinear interactions which are predicted by weak turbulence theory to result in a wave energy spectrum of the form of [k](-2.5). We numerically implement the primitive Euler equations for surface waves and demonstrate agreement between weak turbulence theory and the numerical results.

Journal Article↗

PubMed2002

Peracetic acid as an alternative wastewater disinfectant to chlorine dioxide.

AIMS: The aim of this study was to compare the efficiency of peracetic acid with that of chlorine dioxide in the disinfection of wastewater from a sewage treatment plant (serving about 650 000 inhabitants) that has been using peracetic acid as a disinfectant since 1998. METHODS AND RESULTS: A total of 23 samplings were made, each consisting of three samples: from secondary effluent, effluent disinfected with 2 mg l(-1) of peracetic acid and effluent disinfected with 2.2 mg l(-1) of chlorine dioxide (contact time 20 min). For each sample, measurements were made of the heterotrophic plate count at 36 degrees C, total and faecal coliforms, Escherichia coli, enterococci, pH, suspended solids and chemical oxygen demand (COD). During the first phase of the experiment the peracetic acid was seen to be less efficient than chlorine dioxide. To improve the disinfectant action a system of mechanical agitation was added which led to a greater efficiency in the inactivation of bacteria of faecal origin. CONCLUSIONS: Both products were found to be influenced by the level of microbial contamination, the amount of suspended solids and COD but not by the pH of the effluent before disinfection. The immediate mixing of the wastewater and disinfectant caused a greater reduction in enterococci. SIGNIFICANCE AND IMPACT OF THE STUDY: Since peracetic acid was seen to produce a high abatement of micro-organisms, it can be considered as a valid alternative to chlorine dioxide in the disinfection of wastewaters.

Bacteria↗

PubMed2001

Freak waves in random oceanic sea states.

Freak waves are very large, rare events in a random ocean wave train. Here we study their generation in a random sea state characterized by the Joint North Sea Wave Project spectrum. We assume, to cubic order in nonlinearity, that the wave dynamics are governed by the nonlinear Schrödinger (NLS) equation. We show from extensive numerical simulations of the NLS equation how freak waves in a random sea state are more likely to occur for large values of the Phillips parameter alpha and the enhancement coefficient gamma. Comparison with linear simulations is also reported.

Journal Article↗

PubMed2001

Probability density function and "plus" and "minus" structure functions in a turbulent channel flow.

We consider the statistical properties of the longitudinal velocity increments in a turbulent channel flow at different distances from the wall. The probability density function (PDF) of the velocity difference of the streamwise component near the wall are found to be, especially at small scales, strongly skewed, showing a very long left tail. We consider "plus" and "minus" structure functions and compute separately the statistics for the right and left part of the PDF. It is found that the relative scaling exponents for the right tail are less affected by the presence of the wall and their values are consistent with the ones found in experiments in homogeneous and isotropic turbulence. A simple phenomenological model that explains the results obtained is also given.

Journal Article↗

PubMed2001

Testicular atrophy in 80 HIV-positive patients: a multivariate statistical analysis.

This retrospective study of risk factors for testicular atrophy in HIV-infected men investigates the relationship between complications of AIDS such as wasting or opportunistic illness and testicular atrophy. Microscopic sections of the right testis were evaluated for testicular atrophy by assessing the mean score in each of 80 selected HIV-infected patients who underwent an autopsy during a one-year period. A significant association was observed between testicular atrophy and body mass index (BMI) (P=0.0496). Thus, underweight patients with HIV infection were 3.52 times more likely to have testicular atrophy than those with acceptable body weight. Other significant associations between other variables were not found.

Adult↗

PubMed2000

Small scale intermittency and bursting in a turbulent channel flow

The statistical properties of the streamwise velocity fluctuations in a fully developed turbulent channel flow are studied experimentally by means of single hot wire measurements. The intermittency features, studied through the scaling of the moments of the velocity structure function computed using the extended self- similarity and through the probability density function of the wavelet coefficients, are found to be dependent on the distance from the wall. The maximum intermittency effects are observed in the region between the buffer layer and the inner part of the logarithmic region where it is known that the bursting phenomenon, related to coherent structures such as low speed streaks and streamwise vortices, is the dominant dynamical feature. An eduction technique based on wavelet transform for identification of organized motion is developed and used to analyze the turbulent signals. Streamwise velocity conditional averages computed on events educed with the proposed method are reported. Events responsible for intermittency are found to consist of regions of high velocity gradients and are directly correlated with the observed increase of intermittency close to the wall.

Journal Article↗

PubMed2000

Gallstones in elderly patients: impact of laparoscopic cholecystectomy.

The use of laparoscopic cholecystectomy (LC) in elderly patients may pose problems because of their poor general condition, especially of cardiopulmonary function. Moreover, these patients present with acute cholecystitis and associated common bile duct stones more often than their younger counterparts. From 1990 to 1999, the authors performed 943 LCs; 31 (3.2%) were attempted on elderly patients, 11 (35%) of which were on an emergency basis because of acute cholecystitis, cholangitis or acute biliary pancreatitis. Ten per cent of LCs needed to be converted to an open cholecystectomy, most often because of an increase in the partial pressure of carbon dioxide in the blood produced by excessive operative time. A gasless procedure was used in the last three years of the study on eight cases; the overall rate of conversion from LC to open cholecystectomy in this group was 0%. Associated gallbladder and common bile duct stones were found in five (16%) patients (four preoperative LC endoscopic sphincterotomy and one transcystic approach). The success rate in both of these cases was 100%, overall morbidity was 29% and there was no mortality. These results show that LC is a feasible and safe procedure for use in elderly patients. Gasless LC should be preferred in patients classified as American Society of Anesthesiologists' class III because an excessive duration of operation is the most common reason for converting to an open cholecystectomy.

Aged↗

PubMed1999

Risk factors for colonization or infection due to methicillin-resistant Staphylococcus aureus in HIV-positive patients: a retrospective case-control study.

OBJECTIVE: To determine the risk factors for colonization or infection with methicillin-resistant Staphylococcus aureus in human immunodeficiency virus (HIV)-infected patients. DESIGN: Retrospective matched-pair case-control study. SETTING: Continuity clinic and inpatient HIV service of a university medical center. POPULATION: Patients with HIV infection from the general population of eastern and coastal Texas and from the Texas Department of Criminal Justice. DATA COLLECTION: Patient charts and the AIDS Care and Clinical Research Program Database were reviewed for the following: age, race, number of admissions, total hospital days, presence of a central venous catheter, serum albumin, total white blood cell count and absolute neutrophil count, invasive or surgical procedures, any cultures positive for S. aureus, and a history of opportunistic illnesses, diabetes, or dermatologic diagnoses. Data also were collected on the administration of antibiotics, antiretroviral therapy, steroids, cancer chemotherapy, and subcutaneous medications. RESULTS: In the univariate analysis, the presence of a central venous catheter, an underlying dermatologic disease, lower serum albumin, prior steroid therapy, and prior antibiotic therapy, particularly antistaphylococcal therapy or multiple courses of antibiotics, were associated with increased risk for colonization or infection with methicillin-resistant S. aureus. Multivariate analysis yielded a model that included presence of a central venous catheter, underlying dermatologic disease, broad-spectrum antibiotic exposure, and number of hospital days as independent risk factors for colonization or infection with methicillin-resistant S. aureus. CONCLUSIONS: In our HIV-infected patient population, prior hospitalization, exposure to broad-spectrum antibiotics, presence of a central venous catheter, and dermatologic disease were risk factors for acquisition of methicillin-resistant S. aureus.

AIDS-Related Opportunistic Infections↗

PubMed1999

Endoscopic and surgical integration in the approach to biliary tract disease.

Several techniques are available today to access the bile ducts, all equally safe and effective. Since 1990, we have studied three groups of patients treated with different methods: the sequential endoscopic sphincterotomy + laparoscopic cholecystectomy, the single-stage laparoscopic approach, and the single laparoscopic-endoscopic approach. The results obtained in 127 patients to date suggest that one single-stage treatment is more convenient for the patient, while the combination of endoscopic sphincterotomy with laparoscopic cholecystectomy is preferable in terms of efficacy and safety.

Aged↗

PubMed1997

[Endolaparoscopic "Rendez-vous" in the treatment of cholecysto-choledochal calculosis].

Different methods, all of which equally efficacious and safe, can be selected to access the choledochus in patients with cholecysto and choledocholithiasis on the basis of clinical and anatomosurgical parameters. From 1990 we evaluated three groups of patients who underwent surgery at different times and with different methods: sequentially (endoscopic sphincterotomy and laparoscopic cholecystectomy), one step laparoscopy and combined laparo-endoscopy. The results obtained seem to show that the treatment with laparoscopy alone is the most advantageous in terms of cost-benefit, while the endoscopic access of the choledochus during laparoscopic cholecystectomy is the one to prefer in terms of efficacy and safety.

Biliary Tract Surgical Procedures↗

PubMed1996

[A case of anaplastic carcinoma of the thyroid. Clinico-pathological and therapeutic aspects and review of the literature].

A case of anaplastic carcinoma of the thyroid observed in a 72-year-old female patient, admitted with previous diagnosis of Riedel's thyroiditis, is reported. Clinicopathologic features and management of this rare neoplasm are discussed and Literature is reviewed. Anaplastic cancer is a locally and systemically aggressive histologic type, occurring more frequently in older patients and in those with a history of benign and/or malignant thyroid disease. Because long-term survival was seen most commonly in well-localized anaplastic tumors, a more aggressive approach to thyroid lesions, especially in the elderly, may be warranted to improve early diagnosis and effectiveness of therapy of this nearly always lethal neoplasm. Although patients can rarely be cured, efforts should be made to control the disease locally by combined treatment including surgery and radiochemotherapy, which is found to be, at times, a national palliative therapeutic approach despite patient's advanced age.

Aged↗

PubMed1995

An approach to antiretroviral treatment of HIV disease. New approaches.

Viral resistance limits the value of drugs that act through competitive inhibition of HIV reverse transcriptase (RT). Thus, the emphasis of current research is on agents with other mechanisms. The possibilities include noncompetitive RT inhibitors, drugs against an entirely different enzyme--HIV proteinase, and measures to enhance immunity, either globally or against HIV specifically.

Antiviral Agents↗

PubMed1991

Activation of the granulocyte-monocyte colony stimulating factor gene in acute myeloid leukaemia cells is not related to gene rearrangement.

Several reports have documented that leukaemic blasts produce a number of cytokines among them the granulocyte-monocyte colony stimulating factor (GM-CSF). We analysed the structure of the gene that codes for GM-CSF in 44 acute myeloid leukaemia (AML) cases in an attempt to establish whether the autocrine production of GM-CSF was due to a structural gene alteration. No structural alteration was detected in the GM-CSF gene in any of the 44 cases studied. We, therefore, conclude that the autocrine production of GM-CSF by leukaemia blasts is not dependent on gene rearrangement.

Gene Expression Regulation, Neoplastic↗

PubMed1991

Neuropil threads of Alzheimer's disease show a marked alteration of the normal cytoskeleton.

Abnormal neurites, neuropil threads, are a widespread and characteristic lesion of Alzheimer's disease likely to play a major role in the cognitive impairment of this disease. Contrary to normal neurites, neuropil threads contain straight and paired helical filaments that contain the microtubule-associated protein tau and ubiquitin. It is not known whether these abnormal filaments are added to or replace the normal cytoskeleton. In this study, we examined the fine structure of neuropil threads and carried out a morphometric analysis of the neurofilaments and abnormal filaments contained in the neuropil threads by using an antiserum to tau and colloidal gold immuno-electron microscopy. Almost 70% of the neuropil threads contained straight or paired helical filaments with no neurofilaments. The total number of filaments in each neuropil thread remained essentially unchanged either when straight or paired helical filaments were present alone or when they coexisted either together or with neurofilaments. When the three types of filaments were expressed as a proportion of the total, a linear inverse correlation was found between neurofilaments and straight filaments as well as between straight and paired helical filaments. Approximately 10% of the neuropil threads were found to be myelinated axons. It is concluded that straight filaments are likely to replace neurofilaments, that they in turn might be replaced by paired helical filaments, and that this process occurs in axons as well as dendrites.

Aged↗

PubMed1989

The widespread alteration of neurites in Alzheimer's disease may be unrelated to amyloid deposition.

The structural changes of Alzheimer's disease (AD) include a widespread alteration of neuronal cell processes in addition to senile plaques and neurofibrillary tangles. Since the antigenic characteristics of these abnormal neurites are similar to those of the abnormal neurites associated with the senile plaques, the question has been raised as to whether the widespread neuritic alteration is secondary to the deposition of amyloid. To answer this question, we examined brains from 2 subjects with a longer-lasting form of subacute sclerosing panencephalitis (SSPE) characterized by the presence of numerous neurofibrillary tangles but no senile plaques, 3 subjects with AD, and 2 age-matched controls. Light and electron immunocytochemical analyses revealed that abnormal neurites are present diffusely in SSPE cerebral cortex in the absence of amyloid deposits. These abnormal neurites were qualitatively identical to the widespread abnormal neurites of AD. The abnormal neurites, in contrast to the neurites of control brains, immunoreacted with antibodies to tau and ubiquitin. These distinctive antigenic features were due to the presence in these abnormal neurites of straight filaments, 14 to 16 nm in diameter, mixed with a few paired helical filaments. The spatial distribution of the widespread neuritic alteration correlated with that of neurofibrillary tangles in both conditions, but not with that of senile plaques in AD. The present findings demonstrate that a diffuse alteration of neurites similar to that present in AD takes place independently of the deposition of amyloid in SSPE, and they are consistent with the hypothesis that in AD, also, this alteration is not secondary to the deposition of amyloid.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗

PubMed1989

Alteration of neuritic cytoarchitecture in Alzheimer disease.

Individuals afflicted with Alzheimer disease (AD) demonstrate two prominent brain structural alterations: senile plaques and neurofibrillary tangles (NFT) (Selkoe, 1986). Not appreciated until recently is a third quantitatively significant structure, the dystrophic neurite. Many of these altered neurites are focally associated with amyloid deposits, and together they comprise the senile plaque. However, the great majority of the abnormal neurites in AD are independent of senile plaques (Braak, et al., 1986). Recent advances have yielded considerable information on the chemical nature of the extracellular amyloid (Kang, et al., 1987; Tanzi, et al., 1988) which comprises the central portion of the senile plaque, but there is much yet to be unraveled concerning both the nature of NFT and the relationship between senile plaques and NFT (Selkoe, 1987). While the NFT is principally comprised of 20 nm helically wound filaments, descriptively termed paired helical filaments (PHF) (Kidd, 1963; Wisniewski, et al., 1976) occasionally 12-15 nm straight filaments are also present (Perry, et al., 1987c). We have found that the predominant filaments in dystrophic neurites of AD are straight filaments which are morphologically indistinguishable from those found within NFT. The abnormal neuritic filaments and PHF share microtubule associated protein, tau, and ubiquitin immunoreactivity. The prevalence of altered neurites suggests that reorganization of neuritic cytoarchitecture in AD, as demonstrated by the formation of abnormal polymers structurally, and by inference functionally distinct from the normal cytoskeleton, may constitute a more important part of the cytopathological change of AD than previously appreciated.

Alzheimer Disease↗