Biomedical subjects

M Ou

Publications and source records attributed to M Ou.

8 recordsLinked to original sources

PubMed1999

[The detection of virus antigen in the lower respiratory tract of the patients with lung cancer].

OBJECTIVE: To find out about the viral infection situation of lower respiratory tract of the patients with lung cancer. METHODS: The excretion from the surface of bronchiogenic carcinoma was brushed under fibrobronchoscopy. The respiratory virus antigen was detected and analysed by reagent kit produced by the 262nd Hospital of Beijing Military Region. RESULTS: The respiratory virus antigen was positive in eight cases of lung cancer group, the positive rate was 17.4%(8/46), it was significantly higher than that in non-lung cancer group (P < 0.05). Among them, there were one case of influenza virus A, two cases of influenza virus B, two cases of para-influenza 1,3, two cases of adenovirus and one case of respiratory syncytial virus. The carcinoma accompanied with viral infection were 4,3,1, cases in order of squamous carcinoma, small cell lung carcinoma and adenocarcinoma. CONCLUSION: The results showed that a relationship existed between lung cancer and viral infection of respiratory tract statistically. The viral infection increased in patients with lung cancer, this is worthy to pay attention to.

Adult↗

PubMed1998

p53 overexpression and downregulation of inter-alpha-inhibitor are associated with hyaluronidase enhancement of TNF cytotoxicity in L929 fibroblasts.

Degradation of extracellular matrix by hyaluronidase increases murine L929 cell sensitivity to tumor necrosis factor (TNF) cytotoxicity. Seeding and culturing L929 cells onto the matrix of serum fetuin and the hyaluronate-binding inter-alpha-inhibitor resulted in inhibition of hyaluronidase-enhanced TNF killing, suggesting that the release of these proteins from hyaluronidase-degraded matrix confers cellular TNF susceptibility. Metabolic labeling studies showed that hyaluronidase mediated de novo protein synthesis and down regulated several proteins in L929 cells. Specifically, hyaluronidase upregulated p53 protein expression (>200%) but down regulated a p85 inter-alpha-inhibitor-like protein (>90%) in L929 cells, whereas it had no effect on the protein levels of ICH-1, Bcl-xL, Bcl-2, Fas ligand, CAS (cellular apoptosis susceptible protein), TIAR (an RNA-binding protein) and alpha-tubulin. Conceivably, hyaluronidase enhancement of TNF sensitivity in L929 cells is p53-dependent and the matrix inter-alpha-inhibitor contributes a protective role against TNF cytotoxicity.

Alpha-Globulins↗

PubMed1998

[Factors influencing morale among the elderly in long-term care].

The purpose of this research was to investigate the factors which influence morale among the elderly in long-term care. The sampling criteria for subjects were that they were (1) 65 years old or older and were residents or care recipients at a nursing home, day care or home care center which participated in the experimental project in Department of Health; (2) able to understand the Mandarin or Taiwanese language; (3) capable of verbal communication; and (4) family caregivers whose elder family met the above three requirements. A total sample of 174 subjects consisting of 87 elderly and 87 caregivers were chosen. Research findings revealed: the mean score for morale for elderly in this study was 11.71 out of range 2-19. Except for birthplace and caring models, there was no significant difference among the elderly. The elderly born in Taiwan had significantly higher morale than those born in Mainland China. The elderly in day care had significantly higher morale than those in home care and nursing homes. No significant difference was found among family caregivers, except for family caregivers who had changed their job due to the demands of caring for elderly relations. Elderly in this situation showed significantly lower morale in the elderly whose family caregivers did not change jobs. Correlation analysis showed a positive relationship between the elderly's level of daily activity and their morale level. Higher morale in the elderly was also found to be positively related to perceived higher levels of social support in family caregivers. In multiple regression, morale among the elderly was used as the criterion variable, while the variables of birth place, functional status, caregivers' perceived social support, home care, nursing home, changed job, never having had a job, caregivers' sex caregivers' educational level were used as predictors. The explanation variance achieved using the above research model was 30.78%. Recommendations derived from this study include suggesting the establishment of community adult day care centers to facilitate the elderly being able to stay within their communities, and to provide mental support for caregivers.

Adult↗

PubMed1996

Analysis on decay rate of vibration following impact to human dry skull with and without mouthguards.

The modal analysis on vibration in the human dry skull with and without mouthguards was performed in order to clarify which elasticity modulus of the material and what form of the mouthguard are best. Nine different mouthguards were prepared for this study from three materials with different elasticity moduli amd three different forms: Soft MOLTENO (registered) mouthguards of long, middle, and short forms. Regular MOLENTO (registered) mouthguards of long, middle and short forms, and Hard MOLENTO(registered) mouthguards of long, middle and short forms. Thirty-five measurement points were established on the skull. The main measurement system was composed of a 512-D Vibration Generator, LV-1300 Laser-Doppler Vibrometer, CF-6400 FFT Analyzer, PC-9821Xn personal computer, and Vibrant PC modal analysis software. The decay rate was calculated to evaluate effectiveness of the absorption of vibration of the skull with and without mouthguards when stress was put on the skull. The results showed that he skull fitted with the long and middle mouthguards made of regular MOLTENO (registered) had a higher decay rate than the skull w the mouthguard, and that the material properties of the mouthguards influenced the absorption of the skull vibration more intensively that did the forms of the mouthguards.

Adult↗

PubMed1994

A new method of purification and sensitive bioassay of platelet-activating factor (PAF) in human whole blood.

There is no satisfactory assay procedure of PAF in human whole blood in terms of sensitivity, reproducibility and simplicity. This is due to coexisting lipids from plasma and cellular membranes which inhibit measurement of PAF in various assay procedures, including bioassay. In the present study, an attempt was made to eliminate these interfering lipid inhibitors from blood samples. Lipids in human whole blood were extracted according to the method of Bligh & Dyer and the organic layer was dried under a stream of nitrogen. Then, the dried organic layer was dissolved in diethyl-ether and the solution was kept at -20 degrees C which was then centrifuged. The resulting supernatant was then applied to an anion-exchange column and the PAF fraction was obtained by step-wise gradient elution. The fraction was further purified by normal phase HPLC. Then PAF in the final sample was determined by sensitive bioassay using rabbit platelets containing fibrinogen and epinephrine. The recovery rate of PAF throughout this procedure was constant and satisfactory (37.4 +/- 9.7%), which was confirmed using [3H]-PAF. The lower limit of the present assay was estimated to be 5pg in 1 ml of blood and it was sensitive enough to detect PAF in blood samples from healthy volunteers and patients with sepsis or liver cirrhosis. Furthermore, attempts were made to compare the sensitivity and the recovery of our method with these of a commercially available radioimmunoassay (RIA) kit for PAF. However, it was not possible to detect any amount of authentic PAF added to whole blood.

Animals↗

PubMed1993

[Effect of yin and qi tonifying herbs on hyperthyroid model of yin-deficient rats].

The article reports the effect of Yin and Qi tonifying herbs (Radix Rehmanniae, Radix Ophiopogonis and Pseudostellaria Heterophylla) on hyperthyroid model of Yin-deficient rats. The model rats were made Yin-deficient by injecting thyroxine. Observation showed that after injection, indexes such as anus temperature, oxygen consumption, weight, diet, activity, general conditions, triiodothyronine (T3) and thyroxine (T4) in serum were markedly changed. It is thus suggested that the Yin and Qi tonifying mixture of the above three herbs can regulate and correct Yin-deficiency in hyperthyroid model of Yin-deficient rats.

Animals↗

PubMed1992

Species differences in platelet aggregation induced by platelet-activating factor (PAF).

Species differences in platelet aggregation induced by platelet-activating factor (PAF) were investigated by using the same procedure of platelet preparation and biological assay. Washed platelets of six different species (horses, dogs, rats, rabbits, sheep and guinea pigs) were prepared employing the same method and platelet aggregation was induced by C16-PAF. Horse platelets were most sensitive to PAF (8.0 x 10(-12) M) and rabbit platelets activated by 5.0 x 10(-11) M PAF were also sensitive enough to detect PAF in clinical samples.

Animals↗

PubMed1991

Simple and rapid measurement of platelet-activating factor (PAF) in whole blood.

A simple assay of platelet-activating factor (PAF) in whole blood was developed, employing acetone extraction and thin layer chromatography (TLC) purification of blood sample. The activity of acetylhydrolase present in blood sample was almost completely suppressed by ice-cold acetone extraction, and other inhibitory substances interfering the activity of PAF were effectively removed from the acetone extract by TLC. Then, the treated samples were subjected to a conventional PAF bioassay using rabbit platelets. The recovery rate of PAF by the above procedure was constant and feasible (46-48%). The lower limit of the present assay was estimated to be 1.0 x 10(-10) M. Employing the present method, it was able to determine the amount of PAF in blood (1.2-6.0 x 10(-10) M) of 6 out of 14 septic patients, while no significant PAF activity was detected in the samples from 6 healthy subjects. These results indicate a potential application of the present method in the clinical assay of PAF in blood.

1-Alkyl-2-acetylglycerophosphocholine Esterase↗