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M P Molinari

Publications and source records attributed to M P Molinari.

5 recordsLinked to original sources
PubMed2006

Antimicrobial use and resistance among Gram-negative bacilli in an Italian intensive care unit (ICU).

Gram-negative bacilli antimicrobial resistance remains a significant problem for patients in the intensive care unit (ICU). We performed a retrospective analysis of microbiological data and antibiotic consumption over a 4-year period (2000-2003) in an Italian ICU. Pseudomonas aeruginosa and Klebsiella pneumoniae represented approximately 40% of all isolates. The most significant trend in antimicrobial use was an increase in use of 3(rd )generation cephalosporins, imipenem, and ciprofloxacin. A significant trend toward an increase in resistance rates to piperacillin, 3( rd )generation cephalosporins and ciprofloxacin was observed for K. pneumoniae and a positive correlation between resistance and drug-usage was evident for K. pneumoniae and piperacillin, cefotaxime, ceftazidime, cefepime, and ciprofloxacin, but not for piperacillin/tazobactam. No statistically significant correlations were evidenced for P. aeruginosa. Trends in resistances were studied also for Serratia spp and Proteus spp. Isolation rates of extended-spectrum beta-lactamase (ESBL)-producing strains in pathogens studied were high, especially for K. pneumoniae (72%, 160/222) and Proteus spp (41%, 18/43). In conclusion, the study showed high resistance among Gram-negative organisms isolated in the ICU and significant ESBL production. A significant correlation between antibiotic consumption and increasing resistance was evident for K. pneumoniae.

Anti-Bacterial Agents↗
PubMedDate not supplied

6-(14C)-orotate uptake and rna synthesis by liver from thyroidectomized rats treated with 3,5,3'-triiodo-1-thyronine (t3).

It has been shown that T3 administration to thyroidectomized rats induces a marked increase of the in vivo labelling of liver nuclear RNA by 6-[14C]-orotic acid and, although to a lesser extent, of the radioactivity of the acid-soluble fraction. Therefore, in evaluating the real increase in RNA synthesis induced by T3 administration it has been taken into account that the hormone stimulates the uptake of the labelled precursor by the liver and enhances the specific activity of the nucleoside and nucleotide pool. The greater penetration of orotate into the liver does not depend on the increased RNA synthesis since it is not prevented by the administration of actinomycin D which markedly inhibits the transcriptional process. These findings indicate that the increased uptake of orotate and the stimulation of RNA synthesis represent distinct effects of the thyroid hormone on its target tissue.

Animals↗