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Biomedical subjects

M P Taylor

Publications and source records attributed to M P Taylor.

9 recordsLinked to original sources
PubMed1990

Gentle chemical deoxygenation of hemoglobin solutions.

Dithionite is often used to deoxygenate aqueous solutions because it reacts readily with oxygen. However, milder reducing agents, that do not ordinarily react readily with oxygen, may do so in the presence of an appropriate redox catalyst. We show that dithiothreitol reacts rapidly with oxygen in concentrated hemoglobin solutions to produce a mixture of deoxy-, met- and sulf-hemoglobin. The reaction in neutral phosphate buffer is not significantly affected by superoxide dismutase, benzoate or EDTA. However, addition of catalase or horseradish peroxidase decreases the proportions of met- and sulf-hemoglobin produced. We conclude that both hemoglobin and horse radish peroxidase accept dithiothreitol as the reducing substrate in heme catalyzed reactions with their respective oxidizing substrates (dioxygen and hydrogen peroxide). As a result, deoxy-hemoglobin suitable for physical studies can be prepared with a combination of a stoichiometric excess of dithiothreitol and a catalytic amount of horse radish peroxidase.

Benzoates↗
PubMed1987

Epilepsy in a Doncaster practice: audit and change over eight years.

The results of active management and the findings of repeated audits of epilepsy care over the period 1978-86 in a general practice are described. It was found that about one-fifth of epileptic patients continued to have frequent seizures, usually complex partial, about half had few seizures, and many with mild epilepsy remitted early.By 1980 attempts to reduce polypharmacy, change treatment and achieve optimal use of anticonvulsant drugs in epileptic patients with frequent seizures or side effects had led to an overall improvement in seizure control in 27%, a reduction of polypharmacy in 24% and an improvement in well-being in many patients. Subsequently it proved possible to maintain this improvement, to achieve similar results in epileptic patients joining the practice and to avoid misdiagnosis and polypharmacy in newly diagnosed patients.General practitioners can make a considerable contribution to the care of patients with epilepsy but improved overall care requires better collaboration between neurologists and other clinicians. A district epilepsy service, based on a local clinic, which actively pursues a collaborative approach is suggested as the model for providing optimum care to epilepsy patients.

Anticonvulsants↗
PubMed1977

Continuing education for general practice--a learning system.

A learning system for continuing education for general practitioners is described and illustrated by examples from educational programmes held in Doncaster. The work that needs to be done by organizers in planning, organizing, implementing, and evaluating educational programmes is outlined. I hope that this will help other organizers of continuing education.

Education, Medical, Continuing↗
PubMed1972

Acute myocardial infarction in Doncaster. I. Estimating size of coronary care unit.

A "no refusal" coronary care service (for one year) was offered to a selected sample of 10 general practices (total list 74,657). The patients were admitted to a three-bedded unit in Doncaster Royal Infirmary and data were collected to enable estimation of the size of unit necessary to serve the whole population in the area (census estimate 268,560; total G.P. list 315,811). This estimation was based on:1. The frequency of admission to hospital of suspected acute myocardial infarction in one year, estimated at 978 from the total population.2. The average duration of stay in the unit, which was 2.45 days.3. The distribution of observed occupancy was approximately Poissonian.From these the average expected bed occupancy was calculated as 6.56 and reference to probability tables gave the frequency of overload for different numbers of beds provided. The incidence of acute myocardial infarction in the area is estimated at 275 per 100,000 per annum, on calculations based on the practice list size, and at 323 per 100,000 per annum, on calculations based on census figures.

Adult↗
PubMed1972

Acute myocardial infarction in Doncaster. II. Delays in admission and survival.

The speed of admission of patients with suspected acute myocardial infarction was observed over a period of 12 months during which a "no refusal" coronary care scheme was functioning, with emphasis on minimizing delay. During the same period the duration of survival of cases diagnosed as coronary thrombosis by the coroner's pathologist was measured. Comparison of the two series shows that 75% to 80% of the coroner's cases had died before the median time of notification of the general practitioner by those patients referred to hospital.We argue that the provision of mobile coronary care on request from general practitioners is unlikely to have an appreciable effect in preventing deaths from acute myocardial infarction outside hospital.

Coronary Care Units↗