PubMed Health⌕ Search

Biomedical subjects

M Palkovic

Publications and source records attributed to M Palkovic.

At least 19 recordsLinked to original sources
PubMed2003

[Legal regulations of forensic DNA analysis in the Slovak Republic].

As of 1st January 2003 the Law No. 417/2002 Coll. on the Usage of Deoxyribonucleotic Acid Analysis for the Identification of Persons became effective in the Slovak Republic. The Law sets the conditions to take samples due to carry out the forensic DNA analysis, laid down regulations for laboratories approved to perform DNA analysis and at the same time establishes national database of DNA profiles. The author presents short overview of the Law.

DNA Fingerprinting↗
PubMed1981

Metabolic changes in rats subjected to space flight for 18.5 days in the biosatellite Cosmos 936.

From an investigation of the activity of six glucocorticoid dependent liver enzymes, the existence of chronic, transient, stress-induced hypercorticosteronaemia during flight is probable. This hypercorticosteronaemia arises from weightlessness and induces gluconeogenesis. Weightlessness also caused substantial increases in liver glycogen level. The increased lipolytic activity and that of lipoprotein lipase in several groups of animals could be interpreted as enhancement of fat mobilization and utilization under the influence of stress. As this latter enhancement was also found in ground-based controls, it may have been due to the stress of handling rather than to space flight per se.

Adipose Tissue↗
PubMed1980

The influence of thyroxine on the plasma post-heparin lipolytic activity in rats.

10 min after heparin administration (25 IU per 100 g body weight) the plasma post-heparin lipolytic activity (PHLA) was increased in thyreoidectomized (TECT), hypophysectomized (HX) and hypophysectomized + thyreoidectomized (HX + TECT) rats to the same extent as in intact and sham hypophysectomized controls. However, 60 min after heparin the increase of PHLA persisted only in the TECT, HX and HX + TECT rats. After an administration of thyroxine to TECT rats (25 microgram per 100 g body weight) and also to HX + TECT rats (25 microgram or 100 microgram per 100 g body weight) the PHLA decreased similarly as in controls 60 min after heparin administration. The results suggest that the increased PHLA in hypothyroid rats is caused by the deficiency of thyroid hormones, while TSH released after TECT seems not involved in the regulation of enzymes degrading plasma lipoproteins.

Animals↗
PubMed1977

Effect of premature weaning on the fatty acid composition of rat adipose tissue.

The authors described the effect of premature weaning on the fatty acid spectrum in the retroperintoneal fat of female rats during ontogenesis. Premature weaning reduced the lower fatty acid content [decanoic acid C 10:0, lauric acid C 12:0, myristic acid C 14:0] in 30- to 90-day-old rats, but in further development no differences between the amount of these fatty acids were observed in suckled and prematurely weaned animals. These changes concurred with the fatty acid composition of the relevant diet (rat milk and a standard laboratory diet). Changes induced in the amount of other acids by premature weaning were not permanent in character, with the exception of stearic acid C 18:0 and linoleic acid C 18:3, which were raised at 720 days in the retroperitoneal fat of prematurely weaned rats. The amount of lower saturated fatty acids fell with advancing age, whereas oleic C 18:1, linolic C 18:2 and linolenic C 18:3 acid rose.

Adipose Tissue↗
PubMed1977

Protein determination: a comparison of several methods.

A comparison of four methods for the determination of total proteins is presented from the following points of view: - sensitivity; - specificity; - amount of work, chemicals, time and equipment needed for the performance of the determination. The following tests have been examined; Tombs' (absorbancy at 210 nm); Waddell's (difference in absorbancy between 215 and 225 nm); Warburg's (absorbancy at 280 nm); Lowry's (absorbancy at 500 nm after the reaction with phenol reagent). The authors recommend Tombs' method for its outstanding sensitivity, specificity and simplicity as the best of the four.

Animals↗
PubMed1976

Influence of early weaning on the activity of several enzymes in the liver of rat.

In normally weaned rats the activity of two enzymes in rat liver, i.e. ATP citrate lyase and malic enzyme, gradually increases between the end of the third and the beginning of the fourth postnatal week. The activity of mitochondrial alpha-glycerophosphate oxidase remains unchanged during this period. Early weaning on the 18th postnatal day causes a rapid increase in activity of these enzymes. The impulse for this increase is very probably the change from high fat milk diet to high carbohydrate standard laboratory diet, the decrease of blood lipids, the activation of thyroid gland and changes of sensitivity of hepatic cells towards thyroid hormones. These results showed that early weaning modifies to the known picture of physiological weaning to a great extent.

ATP Citrate (pro-S)-Lyase↗
PubMed1976

Blood lipids during normal and early weaning in rats.

In young rats reared with their mothers, a gradual increase of serum cholesterol, triglycerides, phospholipids and total lipids was found between the 7th and the 18th to 22nd postnatal day. Then the concentrations of lipids gradually decrease. Early weaning at the 18th postnatal day causes a precocious decrease of the concentration of all studied lipids in blood so that their values on days 22 and 26 are significantly lower than in animals weaned on the 30th postnatal day. The body weight of early weaned rats decreases only slightly and returns to normal by the 30th day. The changes in serum lipids after early weaning are caused by differences in the amount of cholesterol and other lipids in breast milk, in comparison with those in the standard laboratory diet. Perhaps they may participate in disturbing the homeostatic equilibrium of cholesterol in early weaned rats.

Age Factors↗