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Biomedical subjects

M Palmieri

Publications and source records attributed to M Palmieri.

At least 109 records · Page 6Linked to original sources
PubMed1971

Cortisone-evoked decrease of acid -galactosidase, -glucuronidase, N-acetyl- -glucosaminidase and arylsulphatase in the ileum of suckling rats.

Changes of activity of intestinal acid beta-galactosidase, beta-glucuronidase, N-acetyl-beta-glucosaminidase and arylsulphatase were studied in suckling rats treated with cortisone (5mg/100g body wt. daily, started on day 9 postnatally) and compared with changes in control animals. Specific activities were not changed within the first 72h, but all enzymes decreased similarly 96h after the first injection. Total activities per ileum and animal were not changed within the first 48h, but within 72h a significant decrease was observed. Calculation of the rate of decrease of the hydrolases studied in cortisone-treated animals shows that it proceeds faster than the rate of renewal of enterocytes in this period.

Animals↗
PubMed1966

Effect of bile on the action of blood against Salmonella.

Kaye, Donald (New York Hospital-Cornell Medical Center, New York, N.Y.), Michelle Palmieri, and Heonir Rocha. Effect of bile on the action of blood against Salmonella. J. Bacteriol. 91:945-952. 1966.-Bile is superior to Trypticase Soy Broth (BBL) as a medium for isolation of Salmonella from blood. In addition to inhibitory activity against many of the common bacterial contaminants, bile has the additional advantages of a greater frequency of isolation of Salmonella and more rapid isolation. This study investigated the factors responsible for the superiority of bile. A 20% dilution of normal human blood in Trypticase Soy Broth was usually inhibitory or bactericidal against strains of Salmonella. Similarly, a 20% dilution in Trypticase Soy Broth of serum from patients with Salmonella bacteremia was frequently inhibitory or bactericidal for the salmonellae isolated from their blood. Bile inactivated the bactericidal activity of blood or serum by inactivating complement activity. Bile (13%) in Trypticase Soy Broth inactivated complement activity in 33% blood or serum. Although bile has anticoagulant and hemolytic effects, no evidence was found that these activities contribute to the superiority of bile over Trypticase Soy Broth as a culture medium for Salmonella. No evidence was found for a growth-promoting factor in bile for Salmonella. Bile was much more effective than 0.5 or 2.0% sodium taurocholate broth in inactivating the bactericidal activity of blood.

Antibodies↗
PubMedDate not supplied

Combined digoxin-molsidomine therapy in congestive heart failure following acute myocardial infarction.

The acute hemodynamic effects of combining administration of digoxin (DIG)(0.01 mg/kg intravenously) with molsidomine (MLS)(4 mg sublingually) were compared with those of DIG and MLS considered alone in 12 patients with congestive heart failure following acute myocardial infarction. The patients were classified into two subgroups, A (cardiac index [CI] less than or equal to 2.2 L/min/m2 and B (CI greater than 2.2 L/min/m2), to verify differences between the responses to the three drug regimens. MLS significantly reduced systolic blood pressure from 121.2 +/- 12.3 (mean +/- SD) to 111.7 +/- 10.9 mm Hg (p less than 0.01) after 60 min, mean right atrial pressure (RAP) from 6.2 +/- 3.6 to 2.4 +/- 2.1 mm Hg (p less than 0.0001), mean pulmonary arterial pressure (PAP), left ventricular filling pressure (LVFP) from 20.6 +/- 2.1 to 12.2 +/- 2.8 mm Hg (p less than 0.0001), and pulmonary vascular resistance (PVR). Left ventricular stroke work index (LVSWI) significantly increased after 60 min. DIG induced a significant reduction in heart rate, RAP, PAP, and LVFP from 20.1 +/- 2 to 14.3 +/- 2.7 mm Hg (p less than 0.0001) after 90 min. Stroke volume index (SVI) increased from 24.7 +/- 4.2 to 27.7 +/- 3.1 ml/beat/m2 (p less than 0.001) and LVSWI from 25.9 +/- 7.2 to 31.9 +/- 5.4 g X m/m2 (p less than 0.0001). The combination of DIG and MLS produced a reduction in RAP, PAP, and LVFP greater than that achieved with either agent alone, with a further shift of the ventricular function curve to the left, thereby leading to an improvement in cardiac performance.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMedDate not supplied

Restoration of tumor specific human leukocyte antigens class I-restricted cytotoxicity by dendritic cell stimulation of tumor infiltrating lymphocytes in patients with advanced ovarian cancer.

Despite the large number of potentially cytotoxic tumor-infiltrating (TIL) and tumor-associated (TAL) lymphocytes accumulated in the peritoneal cavity ascitic fluid and tumor tissue, advanced ovarian cancer is a progressive disease, suggesting that TIL and TAL populations eventually become functionally suppressed in vivo. Dendritic cells (DC) are the most powerful professional antigen presenting cells known in humans and recently, ovarian tumor antigen pulsed DC have been shown to elicit tumor specific human leukocyte antigens (HLA)-class I-restricted cytotoxicity from the peripheral blood of advanced ovarian cancer patients. In this study, we have evaluated the potential of tumor antigen-pulsed fully mature DC stimulation in restoring tumor-specific cytotoxicity in anergic TIL populations from advanced ovarian cancer patients. In addition, we have compared tumor-specific T-cell responses induced by tumor antigen-loaded DC in TIL to those induced in TAL and peripheral blood lymphocytes (PBL). DC stimulation induced powerful cytotoxicity against autologous tumor target cells in TIL-derived CD8+ T-cells from all patients tested, while autologous Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCL) were not lysed. Killing of autologous tumor cells was higher by CD8+ T-cells from TIL compared to PBL and TAL (P < 0.01) and was more strongly inhibited by anti-HLA class I MAb (P < 0.05 compared to PBL and TAL). Phenotypically, all cytotoxic T lymphocyte (CTL) populations were CD3+/CD8+, with variable levels of CD56 expression. Finally, although a marked Type 1 cytokine bias [ie, interferon-gamma/interleukin-4 (IFN-gammahigh/IL-4low)] was observable in all DC-stimulated CD8+ T-cell populations, TIL derived CD8+ T-cells showed a higher percentage of IFN-gamma positive cells compared to TAL and PBL. Taken together, these data show that tumor lysate-pulsed DC can consistently restore strong CD8+ CTL responses from TIL against autologous ovarian cancer cells. DC-stimulated TIL may represent a superior source of tumor-specific CTL for adoptive T-cell immunotherapy for advanced ovarian cancer.

Aged↗
PubMedDate not supplied

[Myxedematous cerebellar ataxia. Description of a case].

The case of a 64-year-old patient with a severe cerebellar ataxia of unknown origin, dating back at least three years, is described. The patient was diagnosed as suffering from hypothyroidism and after one month of replacement therapy with thyroxine there was complete remission both of the myxedema symptoms and of the cerebellar syndrome. The possible association between cerebellar ataxia and myxedema was thus confirmed. The writers stress the importance of identifying this uncommon clinical entity especially in view of the excellent results of thyroxine treatment.

Cerebellar Ataxia↗