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M Paraskevas

Publications and source records attributed to M Paraskevas.

26 records · Page 2Linked to original sources
PubMed1986

Chlamydia trachomatis infection in women with ectopic pregnancy.

Fifty women with ectopic pregnancy and 49 control women with intrauterine pregnancy were interviewed and evaluated for evidence of Chlamydia trachomatis infection. Among women with ectopic pregnancy, 14 women were wearing an intrauterine contraceptive device or had a tubal ligation (group A), and 36 women had no readily identifiable risk factors (group B). Group B women had greater total numbers of sexual partners than did control women with intrauterine pregnancy (P less than .005). Group B women more often had C trachomatis antibody than group A (P = .03) and control women (P = .002). Of 27 C trachomatis cultures from fallopian tube tissue from women with ectopic pregnancy, none were positive. Fallopian tube tissue distant from the site of ectopic implantation was available for histopathology of 41 cases. Nine (22%) had extensive subepithelial plasma cell infiltration. All nine were among group B women (P = .06) and all seven with plasma cell salpingitis who were tested for C trachomatis antibody were seropositive (P = .004). It is concluded that a subset of women with ectopic pregnancy were at increased risk for acquiring a sexually transmitted disease by virtue of their sexual behavior and that women in this subset frequently have serologic evidence of C trachomatis infection and histologic evidence of plasma cell salpingitis. Because few of these women recall having had pelvic infection, the authors speculate that subclinical C trachomatis tubal infection producing plasma cell salpingitis may commonly underly ectopic pregnancy.

Adolescent↗
PubMed1985

The association of sexually transmitted diseases with cervical intraepithelial neoplasia: a case-control study.

Thirty-three women with histologically confirmed cervical intraepithelial neoplasia (grades I to III, with one case of microinvasive carcinoma) and 54 women without evidence of the disease were prospectively studied to determine the relationship of genital infection to cervical neoplasia. Demographic and sexual data for patients and control subjects were collected, with standardized clinical and colposcopic evaluation by means of predefined diagnostic categories. Cultures from the cervix were examined for herpes simplex virus, cytomegalovirus, Chlamydia trachomatis, and Neisseria gonorrhoeae. Human papilloma virus infection was identified by characteristic changes of koilocytosis in cytologic or histopathologic specimen. Cultures from the vagina were evaluated for Gardnerella vaginalis, Trichomonas vaginalis, Ureaplasma urealyticum, Mycoplasma hominis, Candida albicans, and other yeasts. Separate Gram strains were prepared from endocervical secretions and from vaginal secretions. More lifetime sexual partners, larger area of transformation zone, evidence of human papilloma virus infection, and altered vaginal flora were observed in women with cervical intraepithelial neoplasia. The association of human papilloma virus infection and altered vaginal flora with cervical intraepithelial neoplasia was independent of sexual experience.

Adolescent↗
PubMed1983

The cytological diagnosis of prostatic carcinoma by transrectal fine needle aspiration.

During a 2-year period a prospective study was conducted, comparing the accuracy of transrectal fine needle aspiration with that of transperineal needle biopsy in the diagnosis of prostatic carcinoma. With increasing experience the accuracy of aspiration was found to be at least equal to that of biopsy. Blood cultures 1 hour postoperatively in 1 group and 5 minutes postoperatively in another revealed a low incidence of bacteremia. Our findings suggest that prostatic aspiration should be used more widely as the initial diagnostic procedure for suspected prostatic cancer.

Adenocarcinoma↗
PubMedDate not supplied

Laboratory diagnosis of latent human papillomavirus infection.

The etiologic association of human papillomavirus (HPV) with uterine cervical cancer has prompted the need for improved laboratory diagnosis of this virus. The application of conventional hybridization technology, including filter in situ hybridization (FISH) and Southern-blot analysis, has revealed that the detection and typing of the virus is inconsistent between sequential specimens from the same individual. To determine whether the polymerase chain reaction (PCR) can be used to provide a more accurate assessment of infection status, two exfoliated cervical cell specimens obtained sequentially from a cohort of 30 women without clinical evidence of cervical abnormalities were analyzed in parallel by FISH and PCR at 6-month intervals. Neither of the procedures provided consistent findings with two sequential specimens suggesting that multiple analyses are necessary to assess infection accurately. However, PCR was less subjective in interpretation and demonstrated greater specificity than did FISH. With the increased sensitivity inherent to PCR, our findings indicated that PCR is more likely to identify latent HPV infection with a single specimen.

Base Sequence↗
PubMedDate not supplied

Brush vs. spatula for cervical smears. Histologic correlation with concurrent biopsies.

In this study we compared the results of 1,293 brush smears obtained between April 23, 1990, and April 22, 1991, with those of 1,304 consecutive spatula smears from 1989. As expected, 94.1% of brush smears revealed the presence of endocervical cells as compared to 63.04% of the spatula smears (P < .0001). The number of "normal" cases was significantly (P < .001) lower in the brush smears (58.2% for the brush vs. 73.7% for the spatula). A significantly larger number (P < .001) of "abnormal" cases and low and high grade squamous intraepithelial lesions (moderate, severe, carcinoma in situ) were demonstrated in the brush smears. In the second part of the study the results of 547 brush smears with accompanying cervical biopsies obtained during the same period were studied for concordance of interpretation and were compared with 421 biopsy-accompanied spatula smears. Of the brush cases, 17.6% cytologically reported as high grade squamous intraepithelial lesions had low grade squamous intraepithelial lesions or reactive or normal histology. This discrepancy was significantly lower (P < .05) in spatula cases (8.9%). These findings suggest that in this series, although brush sampling yielded a higher pickup, it led to a tendency toward overinterpretation by cytopathologists.

Biopsy, Needle↗
PubMedDate not supplied

Processing fine needle aspiration biopsies for electron microscopic examination. Experience implementing a procedure.

While processing needle rinses from fine needle aspiration biopsy material for electron microscopy, we experienced difficulties not fully outlined previously. Problems in preservation were traced to the time elapsed before glutaraldehyde was added to the needle rinse, which had been held in a physiologic transport medium; a tendency toward hypotonicity of the commercially prepared transport medium; and vigorous handling of the needle rinse. When these problems were dealt with, preservation improved dramatically. The problem of scanty material available for ultrastructural evaluation was dealt with by decreasing the number of smears made and filtering the needle rinse to capture tissue fragments. Implementation of the procedure required leadership, good communication, motivated cytotechnologists and cytopathologists, and continuous supervision and monitoring. In cases with adequately cellular well-preserved material available for electron microscopy, ultrastructural evaluation made a contribution of clinical value to the diagnosis in 48% of cases.

Biopsy, Needle↗