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Biomedical subjects

M Patton

Publications and source records attributed to M Patton.

31 records · Page 2Linked to original sources
PubMed1991

Direct sequence evaluation of the major outer membrane protein gene variant regions of Chlamydia trachomatis subtypes D', I', and L2'.

The nucleotide sequences of variable segments (VS) 1, 2, and 4 for the major outer membrane protein gene (omp1) of Chlamydia trachomatis were determined for serologically defined subtypes D', I', and L2'. Asymmetric DNA amplification was used to produce single-stranded DNA for direct sequencing. Amino acid substitutions were detected in VS1, VS2, and VS4 for I', in VS2 for L2', and in VS4 for D'. DNA sequencing of omp1 variant regions may be an important method for evaluating the molecular epidemiology of Chlamydia spp.

Amino Acid Sequence↗
PubMed1991

Adult polycystic kidney disease in a kindred of West Indian origin exhibits linkage with the 3'HVR probe on chromosome 16.

In a large pedigree of Caribbean origin with adult polycystic kidney disease, linkage has been established to the 3'HVR probe on chromosome 16. Although there are five different fathers in this pedigree, only one of whom was available for DNA analysis, the polymorphic nature of 3'HVR has enabled gene tracking to be carried out. The same allele cosegregates with the disease in every affected family member.

Adult↗
PubMed1991

Use of probes for ZFY, SRY, and the Y pseudoautosomal boundary in XX males, XX true hermaphrodites, and an XY female.

Three XX males, two XX true hermaphrodites, and an XY female were studied for possible deletions using probes for the recently characterised SRY gene and the pseudoautosomal boundary. The XX males and true hermaphrodites were negative for all three probes, while the XY female was positive. One XX male and one XX true hermaphrodite were sibs. A previous sib pair of an XX male and an XX true hermaphrodite have been shown to be positive for Y chromosomal material near the pseudoautosomal boundary. Thus, both phenotypes can be produced from different mutations, some involving the SRY gene and others not.

Adolescent↗
PubMed1989

The virus in our midst.

AIDS and HIV-seropositivity have shown increasing prevalence in the Mountain State. Charleston Area Medical Center (CAMC), being the state's largest tertiary health center, has attracted a number of seropositive patients. This sample, though small by comparison to the experience of other tertiary centers, constitutes the largest single cluster of patients so studied in a single West Virginia hospital to date. This study documents both sociological and economic issues associated with the AIDS population. A clear profile of the typical AIDS patient in West Virginia emerges: 1) gay male; 2) native son; 3) unemployed; 4) Medicaid-dependent; 5) clinically well advanced; 6) infected out of state; 7) under age 30; 8) tertiary care dependent; and 9) homeless. The paper closes with a discussion of the possible growth of the AIDS population in West Virginia in the years ahead. Strategies are set forth to serve as guidelines for managing effectively the AIDS population's needs in both the clinical settings and in society at large.

Acquired Immunodeficiency Syndrome↗
PubMed1983

In vitro perfusion of the isolated dog glomerulus.

Recent technological advances allowing direct in vivo measurements of the determinants of glomerular ultrafiltration have greatly expanded our understanding of that process. In addition, these in vivo studies have clarified the dynamics of glomerular ultrafiltration in a number of physiologic and pathophysiologic conditions. Despite this progress, important issues remain unresolved and beyond the scrutiny of in vivo techniques. We have therefore devised a technique for in vitro glomerular perfusion of the isolated dog glomerulus. In eight glomeruli perfused at physiologic rates, the glomerular filtration rate averaged 39 nl/min and the filtration fraction was 0.19. Filtration pressure disequilibrium was observed in all studies and thus allowed calculation of a unique value for the ultrafiltration coefficient. That parameter averaged 2.34 nl/(min X mmHg). Morphologic studies employing transmission electron microscopy indicate that isolated perfused glomeruli remain ultrastructurally intact. The method for glomerular isolation and in vitro perfusion is presented in detail, the results obtained are compared with published in vivo results, and the advantages offered by the technique are discussed.

Animals↗
PubMed1981

Rifampin prophylaxis for contacts of Haemophilus influenzae type b disease.

Rifampin prophylaxis (20 mg/kg once daily for four days) was used in close contacts of children with Haemophilus influenzae type b (HIB) disease. Two hours after a dose, the concentration of rifampin in serum and saliva exceeded the minimum bactericidal concentrations of organisms obtained from four of the carriers. In both a randomized prospective and an open study, nasopharyngeal carriage was eradicated in 37 (95%) of 39 contacts. No cases of serious H influenzae disease occurred in four to six months of follow-up. At the dose and treatment schedule used, rifampin was safe and effective for eradication of HIB carriage.

Adult↗
PubMedDate not supplied

Regional health assessment relating to mercury content of fish caught in the Yukon-Kuskokwim Delta rivers system.

Seven species of fish were surveyed for muscle tissue mercury content across a broad area of western Alaska. Total mercury levels were determined by cold vapor atomic fluorescence spectroscopy in 66 fish sampled during 1997. Methylmercury in sampled fish amounted to 97 to 100% of total mercury values. Using mercury consumption risk levels derived from U.S. Environmental Protection Agency hazard assessment models, mean total mercury was determined to be above the human critical value of 0.2 g/g (ppm) in 29% of the fish species, and 62% of the fish species contained mercury exceeding the wildlife critical value for piscivorous mammals. Overall, 24% of the fish exceed the critical value for human consumption and 58% the wildlife critical value. Similarly 31% of sites sampled exceeded the human consumption critical value. Based on the mean of all fish sampled and a small number of river otters, a biomagnification factor of 12 was calculated for the Yukon-Kuskokwim Delta Region of Alaska.

Alaska↗
PubMedDate not supplied

The efficacy of dental sealants for an adult population.

A 1-year pilot study was conducted to determine the retentive capacities of dental sealants on adult teeth. It proposed to utilize the knowledge and experience gained in sealant application, as a means of attacking the problem of the increase in restored teeth at risk in the aging population. Eighty patients aged 30-50 had Delton tinted sealant applied on one to four contralateral nonhomologous, first or second, acceptable molar pairs. Fifty-five patients completed the evaluation at 1 year, with a 68% retention for 250 teeth: teeth listed as sound, 94.1%; IC and IC-amalgam, 93.7%, and amalgam, 44.9%.

Adult↗