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Biomedical subjects

M Pegoraro

Publications and source records attributed to M Pegoraro.

At least 19 recordsLinked to original sources
PubMed2001

Interleukin-3 stimulates migration and proliferation of vascular smooth muscle cells: a potential role in atherogenesis.

BACKGROUND: Cytokines released by activated T lymphocytes are key regulators of chronic inflammatory response, including atherosclerosis. The aim of this study was to investigate the presence of interleukin-3 (IL-3) in lymphocytes infiltrating the atherosclerotic plaque and the effect of this cytokine on primary vascular smooth muscle cells (SMCs). METHODS AND RESULTS: Twenty atherosclerotic carotid arterial specimens and 5 early atherosclerotic lesions from the internal carotid were manually minced to fragments, and T lymphocytes infiltrating the atherosclerotic plaque were isolated on solid-phase anti-CD3 polystyrene plates. Southern blot analysis demonstrated that in all samples, lymphocytes expressed IL-3 and IL-2 receptor alpha-chain transcripts, indicating that in this context, the activated T lymphocytes may release IL-3. We further analyzed the expression of the IL-3 receptor and the biological effects exerted by the ligand on vascular SMCs. ss-IL-3-transducing subunit was detected both on cultured SMCs and on endothelial cells and SMCs within atheroma. The analysis of the IL-3-induced biological effects demonstrated that it was able to trigger both mitogenic and motogenic signals. Moreover, we demonstrated that the addition of PD98059, a known inhibitor of the MAP-extracellular signaling-regulated/MAP kinase pathway, completely inhibited IL-3-mediated MAP kinase activation and IL-3-induced migration and proliferation. Finally, IL-3 was found to stimulate vascular endothelial growth factor (VEGF) gene transcription. CONCLUSIONS: IL-3, expressed by activated T lymphocytes infiltrating early and advanced atherosclerotic plaques, may sustain the atherosclerotic process either directly, by activating SMC migration and proliferation, or indirectly, via VEGF production.

Arteriosclerosis↗
PubMed1993

[Charcoal and isoflurane alveolar washout in low-flow circuit].

The inclusion of charcoal filters in anaesthetic low-flow systems contributes to the acceleration of the kinetics of isoflurane (Forane). In twenty subjects, scheduled for extra- and intracranial surgery, ventilated with a low-flow system (Ohmeda Excel OAV7750 with rebreathing cassette) with a mean total flow of 0.7 l/min, awakening was complete within 5 minutes from stopping isoflurane and including charcoal in the circuit. At the awakening, the expiratory concentration of isoflurane was 0.23% (95% confidence intervals: 0.19-0.27%): these values are similar to those of MACawake of isoflurane after fast alveolar washout. The use of charcoal during low-flow anaesthesia makes low-flow systems more flexible in their use; the anaesthetic level is more easily controllable and accidental overdosing of volatile anaesthetics can be immediately removed.

Adult↗
PubMed1990

[Extra-anatomical bypasses: axillofemoral and femorofemoral. Indications and results].

Indications for a unilateral or bilateral axillofemoral and femorofemoral bypass, are presented. Our experience in 48 patients with axillofemoral and femorofemoral form the basis of this report. Indications for these extended bypasses included suppurative growing infection and obliterated common and profunda femoris arteries. These procedures were rapidly performed in critically ill patients. One patient died of recurrent artoduodenal hemorrhage three days following successful axillofemoral bypass. The other patients enjoyed patent grafts for variable periods, although some of them successfully underwent a declotting procedure.

Aged↗
PubMed1990

[Peripheral nerve injuries during carotid endarterectomy].

Our experience with patients undergoing carotid endoarteriectomy over a 10 year period has been retrospectively reviewed. Nerve injuries were detected by reviewing postoperative progress and clinical notes. One hundred thirty-four procedures were performed on 120 patients, to 15 of whom (9%) occurred major nerve injuries. These included seven vagal nerve injuries causing ipsilateral vocal cord paralysis and hoarseness, five injuries of the marginal mandibular nerve and three injuries of the hypoglossal nerve. None of the patients with nerve injury had a stroke as a result of carotid operation. Vocal cord paralysis was documented by laryngoscopy. The incidence of cranial nerve injury during carotid endoarteriectomy appears to be higher than expected, particularly if asymptomatic patients are controlled.

Aged↗
PubMed1990

[Follow-up in femoral artery embolectomy].

The results of 112 femoral embolectomies performed on 100 patients were reviewed. Operative mortality rate was 15% and early amputation (within 30 day-after the procedure) 18%. The role of early amputation was closely related to the time of leg ischemia. Current follow-up as established for 86 patients. 5 years and 10 years survival rates for the group were 49% and 40%. Although early survival is decreased after femoral embolectomy long term survivors can be expected to live independently with excellent limb salvage and function.

Adult↗
PubMed1990

[Popliteal aneurysm. Our experience in 65 cases].

Fifty patients with 65 popliteal aneurysms underwent reconstructive procedures (mean age 64). Atherosclerosis is almost the exclusive cause of popliteal aneurysms. Most of the cases showed an acute ischemia, while he others were asymptomatic or presented a complication of venous occlusion or neural compression. Numerous cases were found incidentally at amputations (these were not included). The best operational method is ligation of the aneurysm sometimes with partial resection and autologous venous graft bypass. The immediate results were excellent in all cases, loss of limb was observed in only three.

Aged↗
PubMed1990

[Aneurysms of the abdominal aorta in patients 80 and over years of age. Long term results].

During a thirteen years period 29 patients 80 years of age and older, underwent surgical procedure for abdominal aortic aneurysm. There were 9 elective aortic reconstructions and 20 ruptured abdominal aortic aneurysms (AAA) repairs. Perioperative mortality after elective AAA resection was 33% vs 50% after ruptured AAA (a postoperative period of 30 days was considered). Comparison of survival following aortic reconstruction in 14 patients with survival of general population matched for age, sex and race, is not significant.

Age Factors↗