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M Pergande

Publications and source records attributed to M Pergande.

At least 37 records · Page 2Linked to original sources
PubMed1988

Different susceptibility of cortical and medullary rat kidney mitochondria to ischemic injury.

Rat kidneys were exposed for different times to in vitro ischemia at 37 degrees C. Then the mitochondria of cortex and medulla were isolated and their respiratory qualities were determined. In dependence on the ischemic time, the active respiration (state 3) and the respiratory control index decreased whereas the resting state respiration (state 4) increased. The cortical mitochondria were affected more strongly by ischemia than medullary mitochondria. Therefore, the different susceptibility of cortical and medullary mitochondria to ischemia does not explain the suggested high risk of medulla for ischemia.

Animals↗
PubMed1987

Diagnostic value of low-molecular mass proteins in serum for the detection of reduced glomerular filtration rate.

The diagnostic value of the low-molecular mass proteins ribonuclease, beta 2-microglobulin, and lysozyme in serum for the detection of reduced glomerular filtration rates was evaluated. The values of these proteins and of serum creatinine investigated in 52 patients suffering from chronic renal diseases were plotted against 99m-Tc-diethylenetriaminopentaacetate clearance as an indicator of glomerular filtration rate. Log-transformed data showed a good fit of linearity. Considering the 95% confidence limits of the regression equations, ribonuclease increased above the normal range when the glomerular filtration rate was lower than 1.24 ml/s whereas the other analytes partly remained within their normal limits. Out of those 18 patients with glomerular filtration rates lower than 1.24 ml/s, all patients showed elevated ribonuclease levels. beta 2-Microglobulin, creatinine, and lysozyme were increased in 17, 14, and 12 cases, respectively. Ribonuclease and beta 2-microglobulin showed similar results when other diagnostic criteria (specificity, efficiency and predictive values) were taken into account. We recommend ribonuclease determination in serum for the detection of reduced glomerular filtration rate in the normal range of creatinine. The test is diagnostically powerful, cheap and easy to perform.

Adult↗
PubMed1985

Influence of cyclosporin A on the respiration of isolated rat kidney mitochondria.

In vitro exposure of isolated rat kidney mitochondria to cyclosporin A, a new immunosuppressive agent with serious nephrotoxic side-effects, leads to alterations of both succinate- and glutamate plus malate-supported respiration in a dose-related manner. ADP- and 2,4-dinitrophenol-stimulated respiration, respiratory control indices, and ADP/O ratios are decreased. The mitochondrial alterations are discussed as possible pathogenetic reasons of cyclosporin A nephrotoxicity.

Adenosine Diphosphate↗
PubMed1985

[Characteristic respiratory magnitude of isolated mitochondria of the rat kidney cortex in relation to measurement conditions].

Respiration speed, respiratory control index and ADP/O-quotient are important characteristics of mitochondria. Since their numerical values much differ in literature, the influence of the composition of the measuring medium on these parameters was established. In their value the parameters are particularly determined by the Mg2+ and the phosphate concentration as well as by the pH-value of the medium. For the investigation of the characteristics a medium of the following composition is recommended: 210 mmol/l saccharose, 10 mmol/l KCl, 10 mmol/l KH2PO4, 60 mmol/l tris(hydroxymethyl)aminomethan (pH 7.40), 0.5 mmol/l ethylene diamine tetra-acetic acid.

Adenosine Diphosphate↗
PubMed1985

Mitochondrial enzymes in human serum: comparative determinations of glutamate dehydrogenase and mitochondrial aspartate aminotransferase in healthy persons and patients with chronic liver diseases.

We measured the activities of two mitochondrial enzymes, the mitochondrial form of aspartate aminotransferase (EC 2.6.1.1) and glutamate dehydrogenase (EC 1.4.1.2), in the serum of apparently healthy persons (n = 84) and patients suffering from chronic liver diseases (n = 43). The distribution of activities for glutamate dehydrogenase, but not mitochondrial aspartate aminotransferase, was sex-dependent. The upper limits of the reference intervals (99th percentile) at 37 degrees C were 3.2 U/L for mitochondrial aspartate aminotransferase, 6.4 U/L for glutamate dehydrogenase (women), and 11.0 U/L for glutamate dehydrogenase (men); there was a weak correlation between the activities of both mitochondrial enzymes (r = 0.439). In patients with chronic liver diseases we found a greater increase in the activity of glutamate dehydrogenase than of mitochondrial aspartate aminotransferase and the correlation between the two mitochondrial enzymes was stronger. The diagnostic sensitivity and specificity of either mitochondrial enzyme was less than that of total aspartate aminotransferase, alanine aminotransferase (EC 2.6.1.2), or gamma-glutamyltransferase (EC 2.3.2.2).

Adult↗
PubMed1985

[Lipid composition of microsomes and mitochondria of the rat kidney cortex].

The lipid content, phospholipid composition and fatty acid composition of the predominant phospholipids were determined in cortical mitochondria and microsomes of rat kidney. Microsomes have a higher lipid content than mitochondria due to their higher content of phospholipid and cholesterol. Both subcellular fractions differ in their phospholipid composition. Phosphatidylcholine and phosphatidylethanolamine form together about 80% and 60% of total phospholipid in mitochondria and microsomes, respectively. The fatty acid patterns of the major phospholipids in mitochondria and microsomes are very similar. Our detailed data will be a well founded basis for studying the influence of pathological events on the lipid composition of kidney.

Animals↗
PubMed1984

Multiple forms of alanine aminopeptidase, alkaline phosphatase and gamma-glutamyltransferase in urine of healthy persons, patients suffering from kidney diseases and patients with kidney transplants.

The catalytic activities of alanine aminopeptidase, alkaline phosphatase and gamma-glutamyltransferase were determined in the soluble and particulate fractions of urine, after ultracentrifugation. In healthy adults the fractional catalytic activities in the supernatants were 0.53, 0.56 and 0.24, respectively. Nearly the same proportions were found in children. In patients suffering from chronic kidney diseases there was a tendency for the proportion of catalytic activity in the soluble fraction to increase. However, the separation into the multiple forms gave no higher diagnostic reliability than the determination of total catalytic activity of the respective enzymes. The determination of multiple forms has no clinical significance in the detection of rejection episodes in renal transplant recipients.

Adult↗
PubMed1984

Characterization of particulate and soluble variants of the brush-border enzymes alanine aminopeptidase, alkaline phosphatase and gamma-glutamyltransferase in human urine.

The urinary brush-border enzymes alanine aminopeptidase, alkaline phosphatase and gamma-glutamyltransferase can be separated by ultracentrifugation into a particulate and a soluble fraction. These variants have been characterized with respect to their kinetic data, heat stability, electrophoretic mobility and behaviour in gel chromatography and affinity chromatography. Both variants of these enzymes show nearly identical Km values and activity curves as functions of rho H and substrate concentration. The particulate variant is more heat-sensitive than the soluble one. The soluble variant as well as the particulate form consist of one fraction bound by Con A-sepharose and another one unbound by it. Origins and nature of these multiple variants in connection with their possible diagnostic significance are discussed.

Alkaline Phosphatase↗
PubMed1983

Stability of enzymes in urine at 37 degrees C.

The activity of alanine aminopeptidase, alkaline phosphatase, gamma-glutamyltransferase, lactate dehydrogenase and beta-N-acetyl-D-glucosaminidase in urine at 37 degrees C was investigated by a model simulating in vivo conditions. The stability of these urinary enzymes is influenced particularly by pH. At low pH values in urine (about pH 5.0) the four first-mentioned enzymes rapidly lose a considerable part of their activity, whereas beta-N-acetyl-D-glucosaminidase is inactivated at higher pH values in urine (about at pH 8.0). This inactivation effect is also time-dependent and can be modified by urinary substances such as creatinine, urea and electrolytes. To avoid misinterpretation of enzyme activity determinations in urine, the simultaneous measurement of urinary pH should be performed.

Creatinine↗
PubMed1983

[Lipid parameters in the perfusate of dog kidneys following hypothermic pulsatile preservation].

The concentrations of the free fatty acids, the triglycerides, the glycerol, and the phospholipids were measured in the perfusate of 10 dog kidneys after hypothermic pulsatile preservation of 48 hours duration. The concentration in free fatty acids decreased in which the fatty acid pattern is unchanged essentially. The free glycerol and the total phospholipids increased. These characteristics of the perfusate refer to an increased lipolysis of neutral fats and a changed membrane composition of the kidney during the preservation.

Animals↗