Biomedical subjects

M Pertseva

Publications and source records attributed to M Pertseva.

3 recordsLinked to original sources

PubMed2003

Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system.

The biological activity of insulins of lower vertebrates (teleosts-Oncorhynchus gorbuscha, Scorpaena porcus, chondrosteans-Acipenser guldenstaedti and cyclostomates-Lamperta fluviatilis) was studied and compared with that of standard pig insulin. The determination of biological activity was made using the novel adenylyl cyclase (AC) test-system based on the adenylyl cyclase signaling mechanism (ACSM) of insulin action discovered earlier by the authors. The biological activity of insulins was estimated as EC(50), i.e. concentration leading to half-maximal activating effect of the hormone (10(-11)-10(-7) M), in vitro, on adenylyl cyclase in two types of the target tissues: in membrane fractions of the muscles of rat and mollusc Anodonta cygnea. In rat, the efficiency of insulins was found to decrease in the following order: pig insulin>scorpaena insulin>gorbuscha insulin>sturgeon insulin>lamprey insulin. In the mollusc, the order was different: sturgeon insulin>scorpaena insulin>pig insulin>gorbuscha insulin. Lamprey insulin at the same concentrations did not apparently reach the maximal adenylyl cyclase activating effect. The suggestion was made that differences in the biological activity of insulins depend on the hormone structure and a number of indexes characteristic of the adenylyl cyclase test-system in the vertebrate and invertebrate tissues. The proposed adenylyl cyclase test-system is highly sensitive to insulin at physiological concentrations, has good reproduction and is easy to apply.

Adenylyl Cyclases↗

PubMed1999

On the mechanism of relaxin action: the involvement of adenylyl cyclase signalling system.

The molecular mechanism of relaxin action was studied taking into account the evolutionary relationship of the peptides belonging to the insulin superfamily and using the authors' previous data on the involvement of the adenylyl cyclase (AC) signalling system in the action of insulin and related peptides. Human relaxin 2 (10(-12)-10(-8) M) has been shown to cause a dose-dependent activating effect on AC in the human myometrium (+370%), in rat skeletal muscles (+117%) and the smooth foot muscles of the bivalve mollusc Anodonta cygnea (+73%). In these tissues mammalian insulin and insulin-like growth factor-1 (IGF-1) also had the AC activating effect. The order of efficiency of the above peptides based upon their ability to induce the maximal AC activating effect was as follows: relaxin > IGF-1 > insulin (human myometrium); IGF-1 > relaxin > insulin (rat skeletal muscle); molluscan insulin-like peptide > IGF-I > insulin > relaxin (molluscan muscle). The relaxin AC activating effect was inhibited with a selective tyrosine kinase blocker tyrphostin 47 and potentiated with Gpp[NH]p providing evidence for the participation of the receptor-tyrosine kinase and G-protein of the stimulatory type (Gs) in the regulatory action of relaxin. The conclusion is that the signalling chain: receptor tyrosine kinase ==> Gs protein ==> AC is involved in the mechanism of relaxin action.

Adenylyl Cyclases↗

PubMed1991

The evolution of hormonal signalling systems.

1. A comparative analysis was made of chemosignalling systems responsible for the action of hormones, hormone-like substances, pheromones, etc. in vertebrates--multicellular invertebrates--unicellular eukaryotes. Many common features revealed in structural-functional organization of the above systems give evidence of their evolutionary conservatism. 2. It was shown that some molecular components as well as signal transduction mechanisms similar to those of higher eukaryote hormonal signalling systems are present in such early organisms as bacteria. This allowed a suggestion that the roots of chemosignalling systems are likely to be found in prokaryotes. 3. The evolution of hormonal signalling systems is discussed in terms of current theories of the origin of eukaryotic cell, its organelles and components. A hypothesis is put forward about endosymbiotic genesis of these signal transduction systems in eukaryotes. 4. A possible evolutionary scenario of the formation of hormonocompetent systems is proposed with hormone-sensitive adenylate cyclase complex taken as an example.

Animals↗