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M Pinard

Publications and source records attributed to M Pinard.

At least 19 recordsLinked to original sources

Radiation-pressure cooling and optomechanical instability of a micromirror.

Recent table-top optical interferometry experiments and advances in gravitational-wave detectors have demonstrated the capability of optical interferometry to detect displacements with high sensitivity. Operation at higher powers will be crucial for further sensitivity enhancement, but dynamical effects caused by radiation pressure on the interferometer mirrors must be taken into account, and the appearance of optomechanical instabilities may jeopardize the stable operation of the next generation of interferometers. These instabilities are the result of a nonlinear coupling between the motion of the mirrors and the optical field, which modifies the effective dynamics of the mirror. Such 'optical spring' effects have already been demonstrated for the mechanical damping of an electromagnetic waveguide with a moving wall, the resonance frequency of a specially designed flexure oscillator, and the optomechanical instability of a silica microtoroidal resonator. Here we present an experiment where a micromechanical resonator is used as a mirror in a very high-finesse optical cavity, and its displacements are monitored with unprecedented sensitivity. By detuning the laser frequency with respect to the cavity resonance, we have observed a drastic cooling of the microresonator by intracavity radiation pressure, down to an effective temperature of 10 kelvin. For opposite detuning, efficient heating is observed, as well as a radiation-pressure-induced instability of the resonator. Further experimental progress and cryogenic operation may lead to the experimental observation of the quantum ground state of a micromechanical resonator, either by passive or active cooling techniques.

Journal Article↗

High-sensitivity optical monitoring of a micromechanical resonator with a quantum-limited optomechanical sensor.

We experimentally demonstrate the high-sensitivity optical monitoring of a micromechanical resonator and its cooling by active control. Coating a low-loss mirror upon the resonator, we have built an optomechanical sensor based on a very high-finesse cavity (30 000). We have measured the thermal noise of the resonator with a quantum-limited sensitivity at the 10(-19) m/sqrt[Hz] level, and cooled the resonator down to 5 K by a cold-damping technique. Applications of our setup range from quantum optics experiments to the experimental demonstration of the quantum ground state of a macroscopic mechanical resonator.

Journal Article↗

Spin squeezing and light entanglement in coherent population trapping.

We show that strong squeezing and entanglement can be generated at the output of a cavity containing atoms interacting with two fields in a coherent population trapping situation, on account of a nonlinear Faraday effect experienced by the fields close to a dark-state resonance in a cavity. Moreover, the cavity provides a feedback mechanism allowing to reduce the quantum fluctuations of the ground state spin, resulting in strong steady state spin squeezing.

Journal Article↗

Long-lived quantum memory with nuclear atomic spins.

We propose to store nonclassical states of light into the macroscopic collective nuclear spin (10(18) atoms) of a 3He vapor, using metastability exchange collisions. These collisions, commonly used to transfer orientation from the metastable state 2 3S1 to the ground state of 3He, can also transfer quantum correlations. This gives a possible experimental scheme to map a squeezed vacuum field state onto a nuclear spin state with very long storage times (hours).

Journal Article↗

Teleportation of an atomic ensemble quantum state.

We propose a protocol to achieve high fidelity quantum state teleportation of a macroscopic atomic ensemble using a pair of quantum-correlated atomic ensembles. We show how to prepare this pair of ensembles using quasiperfect quantum state transfer processes between light and atoms. Our protocol relies on optical joint measurements of the atomic ensemble states and magnetic feedback reconstruction.

Journal Article↗

Continuous variable entanglement using cold atoms.

We present an experimental demonstration of both quadrature and polarization entanglement generated via the interaction between a coherent linearly polarized field and cold atoms in a high finesse optical cavity. The nonlinear atom-field interaction produces two squeezed modes with orthogonal polarizations which are used to generate a pair of nonseparable beams, the entanglement of which is demonstrated by checking the inseparability criterion for continuous variables recently derived by Duan et al. [Phys. Rev. Lett. 84, 2722 (2000)]] and calculating the entanglement of formation [Phys. Rev. Lett. 91, 107901 (2003)]].

Journal Article↗

Polarization squeezing with cold atoms.

We study the interaction of a nearly resonant linearly polarized laser beam with a cloud of cold cesium atoms in a high finesse optical cavity. We show theoretically and experimentally that the cross-Kerr effect due to the saturation of the optical transition produces quadrature squeezing on both the mean field and the orthogonally polarized vacuum mode. An interpretation of this vacuum squeezing as polarization squeezing is given and a method for measuring quantum Stokes parameters for weak beams via a local oscillator is developed.

Journal Article↗

Experimental measurement of the dynamic photothermal effect in Fabry-Perot cavities for gravitational wave detectors.

We report the experimental observation of the frequency dependence of the photothermal effect. The measurements are performed by modulating the laser power absorbed by the mirrors of two high-finesse Fabry-Perot cavities. The results are very well described by a recently proposed theoretical model [M. Cerdonio, L. Conti, A. Heidmann, and M. Pinard, Phys. Rev. D 63, 082003 (2001)]], confirming the correctness of such calculations. Our observations and quantitative characterization of the dynamic photothermal effect demonstrate its critical importance for interferometric displacement measurements towards the quantum limit, as those necessary for gravitational wave detection.

Journal Article↗

A novel regulatory element in the dnmt1 gene that responds to co-activation by Rb and c-Jun.

Rb, c-Jun and dnmt1 play critical roles in the process of cellular differentiation. We demonstrate that a regulatory region of murine dnmt1 contains an element which is responsible for transactivation by Rb and c-Jun in P19 embryocarcinoma cells which is not observed in Y1 adrenocarcinoma cells. During differentiation of P19 cells, the induction of Rb and c-Jun coincides with an increase of dnmt1 mRNA. Using linker scanning mutagenesis we identify the element that is responsible for this activation to be a non-canonical AP-1 site. Our data is an example of how a proto-oncogene activates its downstream effectors by recruiting a tumor suppressor. This interaction of Rb and a proto-oncogene might play an important role in differentiation. The responsiveness of dnmt1 to this type of signal is consistent with an important role for regulated expression of dnmt1 during cellular differentiation.

Animals↗

DNA methyltransferase is a downstream effector of cellular transformation triggered by simian virus 40 large T antigen.

This paper tests the hypothesis that DNA methyltransferase plays a causal role in cellular transformation induced by SV40 T antigen. We show that T antigen expression results in elevation of DNA methyltransferase (MeTase) mRNA, DNA MeTase protein levels, and global genomic DNA methylation. A T antigen mutant that has lost the ability to bind pRb does not induce DNA MeTase. This up-regulation of DNA MeTase by T antigen occurs mainly at the posttranscriptional level by altering mRNA stability. Inhibition of DNA MeTase by antisense oligonucleotide inhibitors results in inhibition of induction of cellular transformation by T antigen as determined by a transient transfection and soft agar assay. These results suggest that elevation of DNA MeTase is an essential component of the oncogenic program induced by T antigen.

3T3 Cells↗

Navigating ambulatory referral: a standardized process.

Significant funding and structure changes to healthcare in Ontario in the mid-90's led The Hospital for Sick Children in Toronto to examine patient referral processes. In an effort to streamline access and encourage more appropriate referrals, the hospital tested and implemented three major changes. This article outlines these changes using the PDSA (Plan, Do, Study, Act) improvement framework and summarizes the results from this project.

Ambulatory Care↗

Feedback regulation of DNA methyltransferase gene expression by methylation.

This paper tests the hypothesis that expression of the DNA methyltransferase, dnmt1, gene is regulated by a methylation-sensitive DNA element. Methylation of DNA is an attractive system for feedback regulation of DNA methyltransferase as the final product of the reaction, methylated DNA, can regulate gene expression in cis. We show that an AP-1-dependent regulatory element of dnmt1 is heavily methylated in most somatic tissues and in the mouse embryonal cell line, P19, and completely unmethylated in a mouse adrenal carcinoma cell line, Y1. dnmt1 is highly over expressed in Y1 relative to P19 cell lines. Global inhibition of DNA methylation in P19 cells by 5-azadeoxycytidine results in demethylation of the AP-1 regulatory region and induction of dnmt1 expression in P19cells, but not Y1 cells. We propose that this regulatory region of dnmt1 acts as a sensor of the DNA methylation capacity of the cell. These results provide an explanation for the documented coexistence of global hypomethylation and high levels of DNA methyltransferase activity in many cancer cells and for the carcinogenic effect of hypomethylating diets.

Animals↗

Inhibition of tumorigenesis by a cytosine-DNA, methyltransferase, antisense oligodeoxynucleotide.

This paper tests the hypothesis that cytosine DNA methyltransferase (DNA MeTase) is a candidate target for anticancer therapy. Several observations have suggested recently that hyperactivation of DNA MeTase plays a critical role in initiation and progression of cancer and that its up-regulation is a component of the Ras oncogenic signaling pathway. We show that a phosphorothioate-modified, antisense oligodeoxynucleotide directed against the DNA MeTase mRNA reduces the level of DNA MeTase mRNA, inhibits DNA MeTase activity, and inhibits anchorage independent growth of Y1 adrenocortical carcinoma cells ex vivo in a dose-dependent manner. Injection of DNA MeTase antisense oligodeoxynucleotides i.p. inhibits the growth of Y1 tumors in syngeneic LAF1 mice, reduces the level of DNA MeTase, and induces demethylation of the adrenocortical-specific gene C21 and its expression in tumors in vivo. These results support the hypothesis that an increase in DNA MeTase activity is critical for tumorigenesis and is reversible by pharmacological inhibition of DNA MeTase.

Animals↗

[Diaper rash].

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Diaper Rash↗