PubMed Health⌕ Search

Biomedical subjects

M Podolak-Dawidziak

Publications and source records attributed to M Podolak-Dawidziak.

At least 19 recordsLinked to original sources
PubMed2003

Serum thrombopoietin levels in patients with reactive thrombocytosis due to lung cancer and in patients with essential thrombocythemia.

In patients with thrombocytosis normal to increased serum thrombopoietin (TPO) levels have been reported. The aim of this study was to investigate the relationship between serum TPO concentration, platelet number and plasma levels of fibrinogen in patients with reactive thrombocytosis (RT) due to lung cancer and in patients with essential (primary) thrombocythemia (ET). A total of 70 newly diagnosed patients with RT or ET (platelet counts >600 G/l) were studied: 45 with RT due to lung cancer (25 with non-small cell lung cancer, NSCLC and 20 with small cell lung cancer, SCLC), and 25 with ET. Twenty normal volunteers were used as controls. TPO was measured by immunoassay technique (ELISA). Mean serum TPO values in patients with RT due to lung cancer were statistically significantly higher than those in patients with ET or in controls. The highest platelet count was seen in patients with ET, and mean plasma fibrinogen levels were the highest in RT patients. In NSCLC patients mean serum TPO concentrations were higher (not statistically significant) than in SCLC, and a statistically significant relationship between TPO serum concentration and fibrinogen level was observed. No correlations between platelet counts and TPO serum concentrations were found. Our results indicate increased serum TPO levels in patients with thrombocytosis in lung cancer which may be related to the activity of neoplasms. In addition, it is postulated that the relatively low TPO values in patients with ET may result from a dysregulation of the feedback loop involved in platelet production.

Adult↗
PubMed1998

[Levels of procollagen type III n-terminal peptide (PIIINP) in serum of patients with myeloproliferative syndrome (mps)].

Serum concentration of aminoterminal peptide of type III procollagen (PIIINP) were determined in 48 patients with myeloproliferative disorders (MPD). 27 with and 21 without marrow fibrosis. Serum concentration of PIIINP in MPD was significantly higher than that in control group, but not in secondary thrombocytosis. The highest serum level of PIIINP was found in the primary osteomielofibrosis. In myeloproliferative disorder a positive correlation between megakaryocyte count, marrow fibrosis and serum level of PIIINP was found. This indicates, that in MPD megakaryocytes stimulates fibroblasts to augment their fibrile formation function. Concentration of PIIINP in the serum seems to be a good, though not specific, marker of marrow fibrosis in myeloproliferative disorders.

Adult↗
PubMed1995

The expression of mRNA for fibrinogen in megakaryocytes isolated from patients with T-cell lymphoma.

The expression of fibrinogen mRNA was studied by in situ hybridization in freshly isolated megakaryocytes in 14 newly-diagnosed patients: seven with non-Hodgkin's lymphoma (NHL), three with immune thrombocytopenic purpura (ITP) and four haematologically normal patients prior to coronary artery bypass surgery. Fibrinogen mRNA in megakaryocytes was not detected in ITP, B-cell lymphomas or in healthy donors. However, it was present in all patients with the high-grade T-cell lymphomas, both with and without thrombocytopenia.

Adult↗
PubMed1995

[Emperipolesis in megakaryocytes in patients with thrombocytosis in the course of myeloproliferative disorders].

The incorporation of other marrow cells into megakaryocytes, termed emperipolesis, has been studied in paraffin biopsy sections from 17 untreated patients with myeloproliferative disorders (MPDs). The group consisted of 12 females and 5 males, aged from 34 to 72 years (mean 51.3). Patients with essential thrombocythemia (ET)--9, chronic granulocytic leukemia (CGL)--4, polycythemia vera (PV)--3, and myelofibrosis (MF)--1 were included into the study. Clusters of large polyploid megakaryocytes were observed in anatomic relation to the marrow sinusoidal system. Emperipolesis has been scored as being present or absent per 100 megakaryocytes/slide. Cells found within megakaryocytes were mostly erythroblasts and mature granulocytes. The number of incorporated cells varied from 1 to 7 per one megakaryocyte. Considering the 17 patients with MPDs, emperipolesis was observed in a vast majority of those with ET(8/9) and PV(2/3), in some with CGL(1/4), but not in MF. The mechanism of megakaryocytic emperipolesis remains unclear. Adhesion molecules on megakaryocytes and incorporated cells may possible mediate the cell-to-cell interactions important for emperipolesis.

Adult↗
PubMed1994

[Treatment of drug induced agranulocytosis with granulocyte-macrophage colony stimulating factor (G-CSF)].

G-CSF was applied in three patients with acute, iatrogenic, immunological agranulocytosis (after ticlopidine, thimazol and aminoglutethimide) complicated by severe infections. Before this treatment was started no improvement had been achieved despite the administration of antibiotics, and corticosteroids for 4 to 9 days. Two patients had anaemia and one--thrombocytopenia probably due to the damage to the earlier, common progenitor cells. In bone marrow smears a plasmocytic reaction reaching 11-13% of total cell counts was observed. After G-CSF all patients showed a prompt amelioration of clinical symptoms, and the leucocyte count raised in several days up to 11.0-73.0 x 10(3)/microliters. Simultaneously young cellular forms of granulocyte lineage appeared in peripheral blood.

Adult↗
PubMed1994

[Susceptibility to roxithromycin of gram-positive cocci isolated from patients with lowered immunity].

The study involved 412 strains of Gram-positive cocci isolated from throat, sputum, urine and stool of patients prepared for bone marrow transplantation at Department of Hematology. Determination of drug susceptibility was performed by disc diffusion method applying discs with roxithromycin, erythromycin, lincomycin, clindamycin and augmentin. Among strains of S. aureus, S. epidermidis and Micrococcus susceptible strains comprised respectively 48, 48 and 45%; among group A, B, C and G streptococci respectively 92 and 52%. Among 108 strains of group D Streptococci only 24% were susceptible to roxithromycin. Comparison of susceptibility of tested strains to roxithromycin and other antibiotics revealed similar susceptibility of staphylococci and streptococci to roxithromycin and erythromycin, while among staphylococci higher percentage of strains and among streptococci lower percentage was susceptible to lincomycin than to roxithromycin.

Anti-Bacterial Agents↗
PubMed1993

[The origin of fibrinogen in megakaryocytes and blood platelets].

Fibrinogen is involved in platelet aggregation and clot formation. It is mainly synthesized by hepatocytes. The additional, small pool of this glycoprotein is known to exist in the alpha granules of bone marrow megakaryocytes and blood platelets. Up-date of the origin of fibrinogen in these cells has been discussed.

Animals↗
PubMed1991

Megakaryocyte progenitors in immune thrombocytopenic purpura (ITP).

Megakaryocyte colony formation was increased in 13 patients with ITP. Out of splenectomized ITP patients Colony Forming Unit-Megakaryocyte (CFU-Mk) number normalized in four but was lower than normal in the remaining two. The proportion of immature megakaryocytes was higher in ITP than in normals or in ITP patients after splenectomy. In conclusion, in ITP accelerated platelet destruction leads to stimulation of megakaryocytopoiesis, but some features of its ineffectiveness can be observed.

Adult↗
PubMed1990

Ability of plasma from patients with immune thrombocytopenic purpura (ITP) to promote the growth of megakaryocyte progenitors from normal bone marrow.

The ability of plasma from ITP patients (before and after splenectomy) to support the growth of megakaryocyte progenitors was compared with that from healthy subjects. Plasma Factor Index-Megakaryocyte PFI-Mk (ITP) which expressed resultant colony growth was significantly lower before splenectomy, but it normalized after splenectomy. (PFI-Mk) (ITP) did not relate neither to megakaryocyte nor to platelet counts. A positive correlation has been observed between megakaryocyte and platelet numbers in healthy subjects and in ITP patients after splenectomy, but not before splenectomy. The proportion of immature megakaryocytes was markedly higher in ITP marrow before splenectomy. This study indicates, that in ITP apart from antibodies directed to platelets and megakarocytes a low plasma stimulatory activity affected megakaryocytopoiesis.

Bone Marrow Cells↗