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Biomedical subjects

M Pokorny

Publications and source records attributed to M Pokorny.

13 recordsLinked to original sources
PubMed1992

Extracellular alpha-amylase from Streptomyces rimosus.

A purification procedure for an extracellular alpha-amylase from Streptomyces rimosus, oxytetracycline-producing strain, is described. The enzyme obtained was shown to be an acidic (pI 4.75) monomer with a relative molecular mass (M(r)) of 43,000, containing three cysteines involved in the catalytic activity of the enzyme. Its amino-terminal part has 57-67% homology with amylases from other Streptomyces species. S. rimosus alpha-amylase is sensitive to higher temperatures, and partially stabilized by Ca2+ ions. It hydrolyses starch (optimum at pH 5.0-6.0) in an endohydrolase manner giving rise to maltotriose, maltotetraose and higher oligosaccharides. Starch granules, except those from rice, were not significantly affected by the isolated alpha-amylase.

Amino Acid Sequence↗
PubMed1992

Early diagnosis of congenital dislocation of the hip.

In this prospective study, 35,550 neonates were examined shortly after birth by a team of orthopaedic surgeons. They diagnosed 775 unstable or dislocated hips in 656 babies; there were two teratological dislocations. Treatment was first with a Frejka pillow and, if this failed to give a normal hip, a Pavlik harness at three months. Early clinical examination did not identify 21 infants who were found to have subluxation or dislocation of the hip at the three-month review. The number of missed cases declined during the study, however, reflecting the increasing experience of the examiners. One case of avascular necrosis occurred in the group treated from birth and one in the late-diagnosed group. Open reduction was necessary only in the two teratological dislocations. Experienced examiners are needed for accurate clinical diagnosis; and treatment should be started before the baby is discharged from the maternity ward.

Czechoslovakia↗
PubMed1991

Comparative determination of salinomycin by high-performance liquid chromatography, microbiological and colorimetric methods in testing production processes and animal feed preparations.

Salinomycin and other polyether antibiotics usually do not have a strong chromophore, nor electrochemical or fluorescence activity. Consequently, common liquid chromatography detectors cannot be used for the direct determination of these substances and LC methods that utilize a specific post-column reaction with vanillin have been developed to overcome this limitation. In this report, some modifications of previously published LC methods were made to optimize the analysis of salinomycin. Comparison with standard microbiological and colorimetric methods has been carried out. The LC method was found to provide the best way of precise, and accurate determination in low levels of salinomycin down to the concentration of 1 mg kg-1.

Animal Feed↗
PubMed1979

Urological injuries associated with pelvic trauma.

We reviewed 100 consecutive cases of pelvic fractures at our hospital to establish the relationship between bone injuries and urological lesions. There were 11 major urological injuries found: 6 patients had bladder laceration, 4 had rupture of the membranous urethra and 1 had a ureteral injury. Emphasis is placed on the physiopathology of the urological injuries.

Adolescent↗
PubMed1976

Ureterostomy in situ: technique for temporary urinary diversion.

Ureterostomy in situ is a simple technique for supravesical urinary diversion. Although it was described a few years ago, it has not gained wide acceptance. From our experience and with slight modifications, we believe there is a place for this procedure as a method of temporary urinary diversion.

Adult↗
PubMed1975

The inhibitory activities of 2-acetamido-2,3-dideoxy-D-hex-2-enonolactones on 2-acetamido-2-deoxy-beta-D-glucosidase.

Treatment of 2-acetamido-2-deoxy-D-mannono-1,4-lactone with dicyclohexylamine in ethanolic solution afforded an unsaturated 1,4-lactone, 2-acetamido-2,3-dideoxy-D-erythro-hex-2-enono-1,4-lactone (1), in good yield. 2-Acetamido-2,3-dideoxy-D-threo-hex-2-enono-1,4-lactone (2) was similarly prepared from 2-acetamido-2-deoxy-D-galactono-1,4-lactone. An unsaturated 1,5-lactone, 2-acetamido-2,3-dideoxy-D-threo-hex-2-enono-1,5-lactone (4), was obtained through the oxidation of 2-acetamido-2-doexy-4,6-0-isopropylidene-D-galactopyranose with silver carbonate on Celite, followed by mild hydrolysis. The inhibitory activity of four isomeric 2-acetamido-2,3-dideoxy-D-hex-2-enonolactones [1, 2, 4, and 2-acetamido-2,3-dideoxy-D-erythro-hex-2-enono-1,5-lactone (3)] was assayed against 2-acetamido-2-deoxy-beta-D-glucosidase from bull epididymis. Only the erythro lactones 1 and 3 are weak competitive inhibitors, whereas the threo lactones 2 and 4 are practically inactive. The 1,4-lactone 1 inhibited 2-acetamido-2-deoxy-beta-D-glucosidase more strongly than the 1,5-lactone 3. The lactones 1-4 were found to be quite stable in aqueous solution or under inhibitory-assay conditions. In addition, two 2-acetamido-2-deoxy-D-glycals, 2-acetamido-1,5-anhydrohex-1-enitol (7) were tested; both are 10 times as active as 1.

Acetylglucosaminidase↗