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Biomedical subjects

M Průcha

Publications and source records attributed to M Průcha.

At least 19 recordsLinked to original sources
PubMed2005

[Immunological monitoring of sepsis using flow cytometry--quantitation of monocyte HLA-DR expression and granulocyte CD64 expression].

BACKGROUND: Sepsis is a serious disease with a high case fatality rate. A variety of changes in the host immune responsiveness are observed in the pathogenesis of sepsis, ranging from detrimental hyperinflammation to profound immunoparalysis, i.e. acquired immunodeficiency. The level of monocyte HLA-DR expression reflects the functional status of monocytes as antigen-presenting cells and granulocyte CD64 expression is also indicative of infectious inflammation. MATERIAL AND METHODS: Monocyte HLA-DR expression and granulocyte CD64 expression were measured in 49 septic patients and 30 healthy controls using flow cytometry focused on three parameters: positive cell percentage, mean fluorescence intensity and quantitation of antibodies bound per cell (QuantiBRITE). RESULTS: The significance of both monocyte HLA-DR expression and granulocyte CD64 expression in septic patients was confirmed. Monocyte HLA-DR dramatically decreases in septic patients compared to controls, is one of the prognostic factors and correlates with C-reactive protein. In contrast, granulocyte CD64 sharply rises in patients with sepsis and correlates with mediators of systemic inflammation (procalcitonin - PCT), proinflammatory mediators (interleukin-6 - IL-6, lipopolysaccharide binding protein - LBP) and anti-inflammatory cytokines (interleukin-10 - IL10). CONCLUSION: Quantitative monocyte HLA-DR expression and granulocyte CD64 expression are useful indicators in septic patients when considered along with the panel of other markers, monitored over a period of time and in the context of the clinical course of sepsis.

Adult↗
PubMed2004

[Evaluation of lymphocyte activation in patients in intensive care units by using flow cytometry for determining expression of early activation antigen CD69].

Disorders in immune reactions represent one of the main pathogenetic mechanisms in patients of Intensive Care Units who suffer from sepsis or syndrome of systemic inflammatory response. The determination of early activation sign CD69 on T lymphocytes makes it possible to evaluate functional capacity of this cell population. The determination of other selected immunological parameters contributes to differential diagnosis of infectious and non-infectious etiology of systemic inflammatory response. The authors demonstrated lower expression of CD69 on CD3+ CD8+ lymphocytes after stimulation with phytohemagglutinin in patients with sepsis as well as patients with the syndrome of systemic inflammatory response of non-infections etiology. No statistically significant differences were proved in the concentrations of IgG, IgA and IgM, number of leukocytes, percentual representation of lymphocytes, TNF alpha production and the expression of surface sign of CD64 in both groups of patients. There were statistically significant differences in the concentrations of C3 and C4 components of complement, prealbumin and procalcitonin. The study did not show correlation between the expression of CD69 and selected immunological parameters (IgA, IgM, IgG, C3, C4, leukocytes, lymphocytes, prealbumin, procalcitonin, TNF alpha and CD64).

Antigens, CD↗
PubMed2003

[Comparison of procalcitonin, interleukin-6 and C-reactive protein in the differential diagnosis of patients with sepsis syndrome in intensive care units].

UNLABELLED: One of the most difficult tasks in differential diagnosis of patients with septic syndrome at the Intensive Care Units is to differentiate between infection and non-infection etiology of this syndrome. In the last years, new parameters have played an important role in this area--C-reactive protein, Interleukin-6 and procalcitonin. THE AIM: Of the investigation was to compare these three parameters in differential diagnosis of the septic syndrome. THE COHORT AND METHODS: The authors examined 56 patients (17 women and 39 men, mean age being 43 and 51 years, respectively) hospitalized at the Intensive Care Units who corresponded to the criteria of the syndrome of inflammatory response, sepsis or septic shock. A total of 99 examinations were done. The samples were taken up to 24 hours after the beginning of clinical symptomatology and submitted to the laboratory within four hours. Immediately afterwards the determination of concentrations of all three parameters--C-reactive protein, interlaukin-6 and procalcitonin, were done. The results of the examinations were compared to each other as well as to the diagnosis of sepsis--the confirmed infection etiology. RESULTS: In all the evaluated parameters the authors detected significant differences between the values of entry examination and all measurements between the patients with the syndrome of systemic inflammatory response and septic shock as well as among patients with sepsis and the septic shock. Likewise, the authors confirmed significant differences between concentrations of all three parameters in comparing the patients with sepsis and those with the septic shock. Only in the case of procalcitonin there was a significant difference in concentration between patients with the syndrome of systemic inflammatory response of non-infectious etiology and those with sepsis. The concentration of procalcitonin was the only predictive marker of diagnosis with the correlation coefficient r = 0.7263, r2 = 0.5275, P < 0.00005. CONCLUSION: Calcitonin proved to be the most specific parameter in demonstrating infection etiology in patients with the septic syndrome, its predictive value being higher than that of C-reactive protein and Interleukin-6. Monitoring of calcitonin dynamism provides important information on efficiency of the applied antibiotic treatment. In patients with diagnostic uncertainties as far as the etiology of the septic syndrome is concerned; procalcitonin is the parameter of choice, while it may be supplemented with the examination of C-reactive protein.

Adult↗
PubMed2003

Complete proteinogram of cerebrospinal fluid and its contribution to the diagnostics of inflammatory and autoimmune diseases of the central nervous system.

Complete evaluation of cerebrospinal fluid proteinogram represents a routine request of the clinician in the analysis of CSF in the Czech Republic. It comprises the measurement of concentrations of acute phase proteins (CRP, orosomucoid, haptoglobin, transferrin, prealbumin), immunoglobulins (IgG, IgA, IgM), compressive markers (albumin, fibrinogen), markers of CNS tissue destruction (apolipoproteins A-I, A-II, Apo B), complement components (C3, C4), alpha-1-microglobulin, beta-2-microglobulin, and proteinase inhibitors (alpha-1-antitrypsin, antithrombin III). Therefore, 19 CSF proteins of precisely verified clinical relevance are routine parameters for the assessment of the functional state of the blood-CSF barrier, presence of the intrathecal synthesis of immunoglobulins, inflammatory changes and verification of CNS tissue destruction. Evidence of these clinically relevant and independent parameters enabled the detection of the presence of autoimmune and neuroinfective diseases of CNS, even in clinical cases where the basic CSF parameters do not express relevant changes, or they are of a bordering or non-specific character. Clinically typical and the most significant abnormalities in the CSF proteinogram represent themselves a new access to a contemporary CSF analysis. Despite the fact that assessment of CSF proteins and their analysis is quite a difficult field in laboratory medicine, it is now routinely requested and routinely performed in the Czech Republic.

Autoimmune Diseases of the Nervous System↗
PubMed1994

[Determination of antiphospholipid antibodies in serum using ELISA].

The authors compare two ELISA methods for the assessment of antiphospholipid antibodies, classes IgG and IgM, in serum: ELISA Pin Plate System ALPHA DIALAB Co. and the ELISA method developed in the Research Institute of Rheumatic Diseases. Both methods use cardiolipin as antigen. In the Pin Plate test the immunochemical reaction antigen/antibody does not take place at the surface of the pits of the microtitration plates but on the tip of the next plate. The results of examinations of antiphospholipid antibodies obtained by the tested methods are comparable, the Pin Plate test is quicker and more sensitive, but its price limits routine use.

Antibodies, Antiphospholipid↗
PubMed1992

[Secondary post-traumatic immunodeficiency syndrome in patients in the anesthesiology-resuscitation department. Possibilities of immunomodulation therapy].

The most frequent cause of death of patients with multiple injuries are infectious complications developing on the basis of secondary posttraumatic immunodeficiency syndrome. The authors submit their experience with monitoring of some immunological parameters of these patients and with the use of immunomodulation therapy with transfer factor--a leucocyte dialysate (TF, DLE, Immodin Sevac). They discuss problems pertaining to the mode of therapy, amounts and time of immunomodulation treatment in these patients.

Adult↗
PubMed1990

[Determination of fibronectin using immunonephelometry].

The authors obtained by a simple isolation procedure pure fibronectin which served as an immunogen for the preparation of the antibody. The antibody was used in the immunonephelometric protein estimation. The authors assessed the reference range of fibronectin concentrations for the healthy population, using different anticoagulating agents.

Fibronectins↗