Biomedical subjects

M Pulat

Publications and source records attributed to M Pulat.

3 recordsLinked to original sources

PubMed1995

Water and antimicrobial agent permation of PU and PHEMA membranes in relation to their surface and bulk properties.

Four types of polyurethane (PU) and two types of 2-polyhydroxyethylmethacrylate (PHEMA) membranes were prepared by convenient methods. Surface properties, water and antibacterial agent permeabilities of PU membranes were investigated. Water permeability values were determined in the region of 2000 g/m2.24 h. The membranes were found to have sufficient permeability toward antibacterial agents of 1% Silver Sulfadiazine and Bacitracin 10,000 UI-Neomycin sulphate 100 mg ointment.

Anti-Infective Agents, Local↗

PubMed1993

Relationship of surface characteristics to cellular attachment in PU and PHEMA.

Adhesion characteristics of fibroblastic baby hamster kidney (BHK) cells and epithelial Madine Darby kidney (MDBK) cells on polyurethane (PU) and polyhydroxyethylmethacrylate (PHEMA) based surfaces have been studied. PU surfaces were prepared by a classical solvent-casting procedure of Pellethane solution that contains different types of solvents, i.e., THF, dioxane and their compositions. PHEMA based surfaces were obtained by bulk polymerization of respective comonomers (HEMA, acrylic acid, AA, and dimethylaminoethylmethacrylate, DMAEMA) in the presence of the crosslinker. Thus, a number of polymeric surfaces were obtained with different surface charges (COO- and NH+4) and with different surface free energies in a range between 60-82 ergs/cm2. Surface properties of these membranes were characterized by equilibrium water contents, air and octane contact angles, surface free energies, SEM photographs and ATR-FTIR spectra. Interactions of BHK and MDBK cells with the surfaces were examined in stationary culture conditions which were carried out in MEM supplemented with fetal calf serum. The observations strongly suggested that the chemical and/or physical properties of membrane surface and morphology of the cell control the degree of cell adhesion to the PU and PHEMA based membranes.

Animals↗

PubMed1990

Structural and cellular characterization of solvent-casted polyurethane membranes.

Fibroblastic cell attachment and growth characteristics of different polyurethane (PU) films were tested. These films were prepared by a classical solvent-casting procedure. By changing the composition and the type of casting solution (i.e. tetrahydrofurane, dioxane, dimethyl formamide-tetrahydrofurane, tetrahydrofurane-dioxane, etc.) PU films with different physical and chemical bulk and surface structures were obtained. Structural properties of these films were investigated by scanning electron microscopy, equilibrium swelling experiments and contact-angle studies. In stationary cell culture tests, a model cell-line, i.e. baby hamster kidney (BHK) were used. Thus the effects of structural properties on the cell behaviour were investigated. The results demonstrate that it is possible to achieve different cell responses by changing the preparation conditions of the films. While the cell attachment is excellent on porous PU surfaces, the others showed similar adhesion. Better proliferation of BHK cells was obtained with PU films prepared from dioxane solution.

Animals↗