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Biomedical subjects

M Putman

Publications and source records attributed to M Putman.

23 records · Page 2Linked to original sources
PubMed1980

Serial studies of leukocyte chemiluminescence: lack of effect of macrolide antibiotic therapy.

Antibiotics which affect protein synthesis have been reported to alter in vitro function of polymorphonuclear neutrophil leukocytes (PMNs). We studied chemiluminescence of PMNs from 30 healthy adults during phagocytosis of opsonized zymosan. Similar chemiluminescence values were obtained before, during, and after oral therapy with erythromycin, rosaramicin, or placebo. While macrolide antibiotic therapy did not alter this PMN function, we conclude that chemiluminescence is a reproducible technique which is well suited for serial evaluation of PMN activity.

Adult↗
PubMed1979

Quantitative nasal cultures from carriers of Staphylococcus aureus: effects of oral therapy with erythromycin, rosamicin, and placebo.

Serial quantitative nasal cultures were performed on 87 healthy nasal carriers of Staphylococcus aureus, who were randomly assigned to 7 days of oral therapy with erythromycin, rosamicin (an investigational macrolide antibiotic), or placebo. Staphylococcal carrier rates decreased during therapy with both antibiotics; however, erythromycin was significantly more effective in lowering carrier rates than was rosamicin. The anti-staphylococcal effects of both antibiotics were similar when the mean numbers of S. aureus isolated from positive cultures during therapy were compared. Side effects to each regimen were minimal.

Adolescent↗
PubMed1977

In vivo effects of josamycin, erythromycin, and placebo therapy on nasal carriage of Staphylococcus aureus.

Healthy nasal carriers of Staphylococcus aureus were randomly assigned to one of three treatment regimens: josamycin (1.5 g/day), erythromycin stearate (1.0 g/day), or placebo, each administered orally for 7 days. Quantitative nasal cultures were obtained from each subject at intervals before, during, and after treatment. All 25 placebo-treated subjects had positive nasal cultures for S. aureus at all culture intervals. Both josamycin and erythromycin were equally effective in reducing the carrier rates and in decreasing the total numbers of S. aureus isolated from subjects with positive cultures during treatment. No increase in in vitro antibiotic resistance was detected in isolates obtained after therapy. Both antibiotics were well tolerated, and toxicity was not encountered with either drug.

Anti-Bacterial Agents↗
PubMedDate not supplied

Multidrug resistance in lactic acid bacteria: molecular mechanisms and clinical relevance.

The active extrusion of cytotoxic compounds from the cell by multidrug transporters is one of the major causes of failure of chemotherapeutic treatment of tumor cells and of infections by pathogenic microorganisms. The secondary multidrug transporter LmrP and the ATP-binding cassette (ABC) type multidrug transporter LmrA in Lactococcus lactis are representatives of the two major classes of multidrug transporters found in pro- and eukaryotic organisms. Therefore, knowledge of the molecular properties of LmrP and LmrA will have a wide significance for multidrug transporters in all living cells, and may enable the development of specific inhibitors and of new drugs which circumvent the action of multidrug transporters. Interestingly, LmrP and LmrA are transport proteins with very different protein structures, which use different mechanisms of energy coupling to transport drugs out of the cell. Surprisingly, both proteins have overlapping specificities for drugs, are inhibited by the same set of modulators, and transport drugs via a similar transport mechanism. The structure-function relationships that dictate drug recognition and transport by LmrP and LmrA will represent an intriguing new area of research.

ATP-Binding Cassette Transporters↗