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M Quilici

Publications and source records attributed to M Quilici.

At least 37 records · Page 2Linked to original sources
PubMed1988

Glomerulonephritis in dogs with canine leishmaniasis.

Kidney specimens, obtained from 11 dogs of diverse species that had contracted spontaneous leishmaniasis, were submitted to a histopathological investigation using a classical preparation of sections embedded in paraffin, semi-thin and ultra-thin sections embedded in plastic, and sections frozen under immunofluorescence. Eight animals presented moderate to severe renal insufficiency. Two major categories of glomerular lesions were identified in the kidneys examined: acute glomerulonephritis and extra-membranous glomerulonephritis. These two categories were never associated and no transition from from one to the other was found. Immune complex deposits prevailed sharply over proliferative phenomena, which remained infrequent. Study of these glomerulonephrities could represent a model for better understanding of the immunological aspects of leishmaniasis.

Animals↗
PubMed1987

[Comparative value of immunoelectrophoresis and ELISA in the serological diagnosis of human hypodermyiasis].

186 sera of patients with hypodermatosis were comparatively studied by immunoelectrophoresis and ELISA. After establishing limits of certainty (2 distinct precipitating arcs with IEP and an optical density greater than or equal to 0.8 with ELISA), the comparison of obtained results shows that there is a good concordance between the two methods, but that the second, by its higher sensitiveness, allows to reveal residual antibodies during a delay after disease recovery longer than with the first. Consequently, this higher sensitiveness allows, first, retrospective diagnosis, then the more frequent settling, when border-lined serological positivities are obtained with IEP, therefore without possible conclusions. On the other side, importance of crossed reactions between Hypoderma bovis and H. lineatum revealed with ELISA, does not allow, contrarily to what is done frequently by IEP, much more specific method, to know the species of Hypoderma causing the disease and, therefore, to bring more to the prognosis.

Enzyme-Linked Immunosorbent Assay↗
PubMed1987

[Optimization of sensitivity tests to ketoconazole. Value of experience planning in the choice of a medium].

Using factorial design, the authors evaluate the factors which influence in vitro, the anti-fungal activity of ketoconazole against a strain of Candida albicans (CBS 562). With the four mediums most frequently used, (Sabouraud, Casitone, MEM, YNB) they precise the results concerning inoculum, pH, incubation temperature, growth time, serum concentration, and shaking. This method allows the evaluation of each factor in optimal conditions of test. In most cases, the best results are obtained with a 10(4) UFC/ml inoculum, a pH = 7, in absence of serum and without shaking. The influence of temperature is very small. In these conditions, the Casitone medium seems the most satisfactory.

Candida albicans↗
PubMed1985

Mediterranean spotted fever in the South of France; serosurvey of dogs.

The authors tested 481 dog sera from the South of France for Rickettsia conorii antibodies using an Indirect Immunofluorescent Antibody Technique. Eighty percent of the sera were positive at a dilution of 1:32 and 45% at a dilution of 1:128. The authors conclude that this provides evidence of the endemic situation of Mediterranean Spotted Fever in the South of France.

Animals↗
PubMed1985

[Trichinosis in Provence. Apropos of a familial epidemic].

An outbreak of trichinosis following meals in a rural area of Provence is reported. The epidemiologic parameters reconstitute the contamination cycle (man-pig: fox-rat) which indicated indisputably the diagnosis, the appropriate therapy, and also evidenced a rural zoonosis of trichinosis in Provence. The clinical picture among twenty-one people included morbilliform rash in 38% of cases and an enanthema in 24% of cases, uncommon findings, with respect to their frequency, and the other usual signs. Early laboratory diagnosis was made possible by blood analysis for hypereosinophilia and immunoassay (ELISA) for antigen-specific IgE. Treatment with benzimidazoles was quite effective.

Adult↗
PubMed1983

[Hydatic cyst of the pelvis. Failure of treatment with flubendazole].

In a 54 year old, algerian male we found a inoperable hydatid disease of the pelvis. A treatment by flubendazole 4 g/day is started on February 1982. The patient presents a neck of the femur fracture, on April 1982; on September 1982 he had a myocardial infarctus and a second one in February 1983. On March 1983 he had a new femur fracture. The biological data show no difference in hypereosinophilia and serological exploration. In this case the result is poor.

Administration, Topical↗
PubMed1982

[Human brucellosis in Mali. Results of a seroepidemiological study].

Prevalence of human brucellosis was evaluated in three contrasted ecoclimatic zones with different peopling of Mali. Rose bengal plate agglutination tests were carried out on capillary blood microsamples taken from 2173 subjects and indirect immunofluorescence tests were performed on 148 blood samples collected on filter paper disks. These seroepidemiological studies demonstrate the presence of the anthropozoonosis in the entire country and the influence of ecoclimatic and demographic factors on human brucellosis distribution in Mali. The high prevalence (24.4%) found in the sahelian region of Gourma shows the necessity of a national control programme. A similar prevalence has been found in other countries of tropical Africa which were formerly considered as almost free of human brucellosis. The findings suggest that seroimmunological tests should be performed systematically to detect brucellosis in immigrants coming to Europe from tropical Africa as well as in Europeans who have been travelling or staying in this region.

Agglutination Tests↗
PubMed1982

[Enteropathogenic mechanisms involved in giardiasis in children (author's transl)].

23 patients with gastroenteritis and 9 with severe malabsorption syndrome related to giardiasis were investigated in a semi-prospective fashion as follows: (1) conjugated bile acid levels measured in duodenal aspirate (thin layer chromatography) in 6 patients with steatorrhea. (2) intraepithelial lymphocytes count (results expressed as the number of intraepithelial lymphocytes per 100 epithelial cells) in small intestinal biopsies from the 32 patients, 11 of which had immunoglobulin deficiency (9 IgA deficiency). The results indicate that there is no decrease in the percentage of conjugated bile acids (mean percentage 90%; normal = 80); a significantly increased percentage of intra-epithelial lymphocytes is documented in giardiasis (11.1% +/- 6.7), versus 2.3% +/- 0.5 in acute gastroenteritis (9 patients) and 6.3 +/- 0.5 in chronic diarrheas (6 patients) (p less than 0,001). This percentage, however, is significantly lower than in untreated coeliac sprue (23 patients) (12.17 +/- 11.6) (p less than 0,01). Conversely a high intraepithelial lymphocyte count does not correlate with the degree of intestinal villous atrophy (3 patients had severe and 6 partial villous atrophy) (r = 0.170). IgA deficiency should be suspected in patients with giardiasis presenting with intestinal villous atrophy (5 patients). Steatorrhea in our patients does not appear related to bile acid deconjugation. To explain enterotoxicity in giardiasis, more than a direct effect of the ventral disk of the parasite on intestinal mucosa, one should incriminate the host immune cell mediated response as shown by lymphocytic infiltration of the epithelium on small bowel biopsies.

Adolescent↗
PubMed1981

[Growth of Leishmania infantum in continuous culture].

It has been studied the continuous growth of a Leishmania infantum canine strain from Marseille. The authors used a modified liquid medium with Panmed : foetal calf serum and calf serum. They used a bench to chemistat at 24 degrees C. With an optimum concentration inoculum they obtained a diphasic growth curve with a first exponential phase from the third to the fifth day, in which the average time of generation is 15 hours and 20 minutes, and a second growth phase from the ninth to the eleventh day. It is a--diauxie growth--(according to the J. Monod Theory). If some medium is added on the third day this first exponential growth phase continues up to the eleventh day.

Adaptation, Physiological↗
PubMed1980

Is the HpO phenomenon in tropical populations really genetic?

Long-term studies of 98 unselected adults from a rural African community in Mali where malaria is endemic showed that the haptoglobin level of the blood increased significantly after 1 year of continuous anti-malarial treatment. This seems to indicate that hypo- or anhaptoglobinaemia in populations like this may in a considerable number of cases have a non-genetic basis.

Adult↗
PubMed1979

[Results of a year's mycologic and immunologic surveillance of Candida in an intensive care ward].

Patients hospitalized in an intensive care unit must be considered to be exposed to a "high risk" in terms of candida infection. They often combine several of the factors favouring the development of "candida disease": generally deficient state, multiple antibiotics, iatrogenic or pathological immune depression. While certain patients who are admitted are already suffering from "candida infestation" (endogenous localization), the possibilities of exagenous infection are numerous. This study involved 63 patients spending at least one week in the department, from September 1977 to September 1978. Mycological (pharyngeal swab, urine culture, blood culture) and immunological (hemagglutination, immunoelectrophoresis, immunofluorescence) studies were carried out routinely on admission and then every week. Amongst the 63 patients studied, 12 were admitted with negative serology, which became positive during the first 10 days. 5 already had positive serology at the time of admission. From a mycological standpoint, the pharyngeal swab was frequently positive on admission (17 times out of 63). In 63 patients, the infection was acquired during the course of the hospital stay and, in this case, positive urine cultures were more regularly associated with a positive pharyngeal culture.

Candida↗