AN ADAPTOR FOR MOUNTING GLASS KNIVES.
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Equipment and Supplies↗
Biomedical subjects
M R CLEVENGER
Publications and source records attributed to M R CLEVENGER.
ULTRAMICROTOME: A SIMPLE, EASILY CONSTRUCTED INSTRUMENT.
A practical microtome may be built with nominal skill, hand tools, and a drill press. Thermal specimen advance and cutting action by rod flexion eliminate pivots and critical moving parts. The cost of materials is negligible. Resultant sections are adequate for routine work in electron microscopy. sectioning for electron microscopy.
Equipment and Supplies↗
Polyester-methacrylate embedments for electron microscopy.
It has been found that tissues fixed for electron microscopy and dehydrated in acetone can be embedded in mixtures of n-butyl methacrylate and polyester resin. Activation with 1 per cent tert-butyl hydroperoxide followed by 12 to 48 hours at 60 degrees C produces blocks that section well with glass knives. The ribbons are cleared of methacrylate by heat (200-250 degrees C for 1 hour) and/or immersion in organic solvents (CCL(4), acetone-ether). After removal of the methacrylate the residual polyester matrix provides thermostable and insoluble support for the tissue. Its insolubility permits staining by immersion of cleared preparations in organic solvents carrying heavy metal compounds in solution. Clearing by heat stabilizes section-grid relationships. The removal of volatile materials by clearing substantially reduces contamination of both specimen and microscope. Tissue fine structure is well preserved in these preparations.
Electrons↗