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Biomedical subjects

M R Ras

Publications and source records attributed to M R Ras.

29 records · Page 2Linked to original sources
PubMed1977

Evidence of agar gel electrophoresis changes of lipoprotein-X after phospholipase A and deoxycholic acid.

The effect of phospholipase A from snake venom and deoxycholic acid on lipoprotein-X (LP-X) recovered from the cathode side of a previous agar gel electrophoresis is described. Adding phospholipase A and deoxycholic acid to the removed cathodal fraction is followed by a marked migration to the anode side on a second electrophoresis procedure. This seems to confirm that phospholipase A and bile salts on LP-X particles modifying their agar gel electrophoretic migration characteristics.

Cholestasis↗
PubMed1977

Liver tissue lecithin: cholesterol acyltransferase activity in patients with liver disease.

Lecithin: Cholesterol acyltransferase (LCAT) activity was measured in serum and liver tissue from patients with parenchymal liver disease. Serum LCAT activity was within normal limits and it was probably related to the absence of clinical and laboratory evidence of a decompensated liver function. Liver tissue LCAT activity is about tenfold lower than that in serum. The relative proportion of cholesterol esters in liver tissue was much higher that could be expected according the low tissue LCAT activity. This findings suggest that LCAT is a "plasma specific" enzyme and that cholesterol esters in parenchyma may be considered as a storage form of cholesterol esterified in plasma pool.

Acyltransferases↗
PubMed1976

Effect of heparin on lecithin: cholesterol acyltransferase and lipids in cholestasis.

The effect of heparin on the proportion of cholesterol esters, lecithin/lysolecithin ratio, and lecithin: cholesterol acyltransferase (LCAT) activity was studied in twelve patients with cholestasis. A good correlation was observed between a decreased LCAT activity and the proportion of cholesterol esters, and no consistent variation was noted after heparin. The lecithin/lysolecithin ratio was increased in 7 patients and became normal after heparin in 6 cases. The clearance of lipoprotein-X after heparin seems to be not related to LCAT activity. These results demonstrate that heparin does not modify the LCAT activity, and suggest that heparin induces an increase of phospholipase activity in some patients with cholestasis.

Acyltransferases↗
PubMed1976

Action of heparin on lecithin:cholesterol acyltransferase activity in normal subjects.

The triglyceride decrease and free fatty acid increase by lipoproteinlipase post-heparin effect does not modify the Lecithin:Cholesterol Acyltransferase activity in vitro, using homologous substrate, in normal subjects. These findings agree with the unmodified proportion of esterified cholesterol and relative proportion of phospholipids on thin layer chromatographic fractioning after heparin. The conclusion is reached that heparin has no action on Lecithin:Cholesterol Acyltransferase activity in normal subjects.

Acyltransferases↗
PubMed1976

[Determination of lecithin: cholesterol acyltransferase activity by a radioisotopic assay (author's transl)].

A method for determining Lecithin: Cholesterol Acyltransferase (LCAT) activity is presented. Trace amounts of labelled cholesterol are added to innactivated homologous substrate from a pool of normal serum. LCAT activity is determined measuring the cholesterol esterification, and is expressed as micrograms of cholesterol esters formed per milliliter plasma per hour. LCAT time-course, reproducibility, activity after serum storage at 4 degrees C, and normal values from 20 healthy subjects are studied.

Acyltransferases↗
PubMed1975

The effect of heparin on serum lipoprotein-X.

Serum lipoprotein-X was investigated in 12 patients with cholestasis in basal conditions and 20 minutes after intravenous heparin. In all cases, lipoprotein-X was positive in the first sample and became negative after heparin. A decrease in triglycerides was observed in all patients after heparin with an increase in free fatty acids and mobilization of prebetalipoproteins, and a transformation of lecithin into lysoleithin in 4 cases after heparin. These facts suggest that heparin plays an important role in the mobilization of serum lipoprotein-X.

Adult↗