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Biomedical subjects

M Röthlisberger

Publications and source records attributed to M Röthlisberger.

10 recordsLinked to original sources
PubMed1999

Molecular screening for alkane hydroxylase genes in Gram-negative and Gram-positive strains.

We have developed highly degenerate oligonucleotides for polymerase chain reaction (PCR) amplification of genes related to the Pseudomonas oleovorans GPo1 and Acinetobacter sp. ADP1 alkane hydroxylases, based on a number of highly conserved sequence motifs. In all Gram-negative and in two out of three Gram-positive strains able to grow on medium- (C6-C11) or long-chain n-alkanes (C12-C16), PCR products of the expected size were obtained. The PCR fragments were cloned and sequenced and found to encode peptides with 43.2-93.8% sequence identity to the corresponding fragment of the P. oleovorans GPo1 alkane hydroxylase. Strains that were unable to grow on n-alkanes did not yield PCR products with homology to alkane hydroxylase genes. The alkane hydroxylase genes of Acinetobacter calcoaceticus EB104 and Pseudomonas putida P1 were cloned using the PCR products as probes. The two genes allow an alkane hydroxylase-negative mutant of Acinetobacter sp. ADP1 and an Escherichia coli recombinant containing all P. oleovorans alk genes except alkB, respectively, to grow on n-alkanes, showing that the cloned genes do indeed encode alkane hydroxylases.

Acinetobacter calcoaceticus↗
PubMed1998

[Pedestrian accidents].

The epidemiology of non-traffic-related, pedestrian injuries in the winter sports resort of Arosa (Switzerland) between 1968 and 1995 (beginning december to end april) was studied. A total of 2813 patients (1140 male and 1673 female patients) with 3010 injuries was surveyed in a general practice. The study showed in the examined period not only an increasing number of injuries and a culmination in February, but also a higher risk for females (60 versus 40 per cent) and an increasing incidence with age. 6% of all patients had more than one injury and 5% required hospitalization. Most pedestrians had injuries of the upper and lower extremity; fracture of the distal radius was the most often diagnosed injury, followed by distortions of the foot and wrist and fractures of the ankle. For accident prevention it is important, that the authorities do not abstain from mechanical cleaning of walking surfaces as soon as they become slippery and from giving out warnings via the mass media. For outdoor walking we postulate boots for ankle protection. The results are discussed and compared with already existing publications.

Accidents, Traffic↗
PubMed1996

[Comparison between ski and snowboarding accidents. Current overview from the ski area Arosa (Switzerland) (1988/89 to 1994/95)].

The ski resort of Arosa (Switzerland) is geographically isolated and has recorded it winter sports accidents since 1969. Based on this reliable data, the ski (3877 in total) and snowboard (512 in total) accidents of the last seven years were statistically analyzed. The study showed that snowboarding isn't much more dangerous than skiing the number of accidents was about the same in both groups, whereas snowboarders were less likely to be admitted to a hospital or to get injured by collision. The average age of snowboarders was 21.6 years, almost 20 years lower than that of the skiers. Both sexes were about equally represented. Most snowboarders had injuries of the upper extremity (14% fractures of the radius, 9% distortions), particularly because of falling backward after losing balance. In both groups (skiers and snowboarders) 60% of all injuries were of low grade, which did not lead to long absence from work. The results are discussed and compared with already existing publications.

Accidents↗
PubMed1990

[Changes in skiing accidents during the last 20 years].

Since 23 years the skiing-accidents are statistically surveyed in the private practice of the author. The number of injured patients is continuously decreasing. Specially the injuries in years with little snow fall are analyzed. It is evident that leg fractures are quite rare but severe injuries of the knee are increasing. 50% of ski accidents are minor injuries.

Accidents↗
PubMed1986

Iodination of thyroglobulin molecules depends on their diffusion velocity in follicular colloid.

We have studied in vitro the effects of altered physicochemical properties of thyroglobulin molecules in solution and of the solution itself on iodination kinetics and hormone synthesis. Any change in hydrodynamic properties had a far greater effect in compartmentalized systems, obtained by coating the test tubes with peroxidase, than in conventional homogeneously mixed systems. Increasing thyroglobulin concentration in a range still far below that existing in vivo greatly retarded iodination and hormone synthesis. In contrast, a number of physiological and non-physiological changes of thyroglobulin structure, such as desialylation, preiodination, oxidation and denaturation, strikingly accelerated iodination. The highly variable physical-chemical state of thyroglobulin molecules appears to be a main determinant of protein diffusion within the colloid and, thereby, of iodination kinetics and rate of hormone synthesis. Moreover, alterations of the physicochemical state of thyroglobulin molecules may explain some hitherto ill-understood diffusion phenomena in live follicles.

Chemical Phenomena↗
PubMed1981

[Skiing accidents in children (author's transl)].

In Arosa, a coherent skiing arena, all skiing accidents were systemically recorded by the two general practitioners for 10 years. The incidence of accidents to children compared with that of adolescents and adults was of particular interest. The incidence of accidents to children has more markedly decreased. The frequency of collisions is increasing. The most common injuries to children are fractures, followed by sprains and contusions. On the other hand sprains are the most common injury to adolescents and adults, followed by fractures and an almost equal number of contusions. The dangers of skiing and the severity of the injuries are discussed. Demands are made for prevention of skiing accidents.

Adolescent↗