Biomedical subjects
Publications and source records attributed to M Raska.
The article summarises current knowledge about the preparation and standardisation of treatment and diagnosis of allergies. It summarises the basic conceptions, units, methods and conditions for preparation, conservation and the storage of allergens. The article is especially orientated towards grass pollen.
The review concerns heat shock proteins and their significance in immune reactions. It focuses on problems of physiological and pathological interactions in etiology and duration of autoimmune diseases and infection processes, especially fungal infections. New trends are described in exploitation of heat shock proteins for preparation of specific protective vaccines.
Three sequences of hsp60 from Saccharomyces cerevisiae, Schizosaccharomyces pombe and Histoplasma capsulatum were compared. Local multiple alignment of these sequences allowed the selection of two oligonucleotides suitable as primers for the polymerase chain reaction. This primer set was used for the amplification of a part of the hsp60 gene from cDNA of Trichophyton mentagrophytes and S. cerevisiae. Similar fragments detected in both PCR's imply the possible future use of the developed primer set for the detection of the hsp60 gene in other fungal species.
Five mutants of Trichophyton mentagrophytes with changed optimum cultivation temperature and the wild strain were used in this study. The growth rates, the keratinolytic activities and the 1-D electrophoretic patterns of these mutants were examined repeatedly. The differences among the individual mutants and the wild strain can be useful for a better understanding of the participating individual proteins for thermotolerance of this fungus.
Protein pattern changes were investigated in the filamentous fungus Trichophyton mentagrophytes during the morphological transition induced by increased temperature and higher CO2 partial pressure in cultivation atmosphere. The differences between the mycelial and the arthroconidial phase were characterized by SDS-PAGE and by immunodetection with mouse polyclonal antibodies. The components found by Western blotting in mycelia were 88, 86, 32, 29, 19.5, 18.5 kDa, in arthroconidia 108, 92, 88, 66, 56, 41, 39, 19.5 kDa. The results suggest the participation of some heat shock associated proteins of T. mentagrophytes in host immune response against mycotic infection.