PubMed Health⌕ Search

Biomedical subjects

M Regan

Publications and source records attributed to M Regan.

10 recordsLinked to original sources
PubMed1991

Electron microscopic characterization of neonatal platelet ultrastructure: effects of sampling techniques.

Detailed ultrastructure of 720 neonatal platelets was systematically examined in 200-300 microliters cord blood samples from four normal term infants. There were no differences in platelet organelles between samples collected into standard sodium citrate anticoagulant and minimally heparinized samples collected into sodium citrate. Samples collected into EDTA showed significant differences from the other two solutions. There were also differences in organelle characteristics within a solution group, as time from sample collection to EM fixation varied. These results verify utilization of small minimally heparinized blood samples for examination of platelet ultrastructure. This system can now be expanded to the premature infant, where small blood sample requirements are essential, and minimally heparinized blood may be more readily available.

Anticoagulants↗
PubMed1991

Occupational hearing loss in New York dairy farmers.

A study of 49 randomly selected, full-time dairy farmers was performed to assess the prevalence and nature of hearing loss in this population. An age- and sex-matched group of rural, non-farmers was also studied. Medical, occupational, and recreational histories were taken and standard audiometric testing was administered. Sixty-five percent of farmers had hearing loss in the higher frequencies and 37% had losses in the mid-frequency range as compared to 37% and 12% of non-farmers respectively (p less than .01). Farmers' left ears were more severely affected. The age of the subjects and the number of years spent farming were highly correlated with hearing loss. Correlation and regression analyses supported the hypothesis that the difference in the prevalence of hearing loss between the dairy farmers and the non-farmers was due to occupational noise exposure on the farm.

Adult↗
PubMed1991

A proposed minimal rheumatological screening history and examination. The joint answers back.

We have developed a rapid, reliable locomotor screening procedure to identify regional locomotor problems. An initial screen was tested in general medical inpatients and modified in the light of this experience. The revised screen was subsequently retested in a similar group of patients and new referrals to a rheumatology outpatient clinic. The revised screen proved sensitive, quick, and acceptable to both patients and doctors. Such a screen could readily be adopted by undergraduates: its routine use should improve detection and awareness of rheumatological problems and form the foundation for development of rheumatological clinical skills.

Adult↗
PubMed1990

Histopathologic features of composite ganglioneuroblastoma. Immunohistochemical distinction of the stromal component is related to prognosis.

Histopathologic features of 18 cases of composite ganglioneuroblastoma (CGNB) were studied with immunohistochemical staining techniques using antibodies against S-100 protein (S-100), ferritin (FER), and leukocytic common antigen (LCA). Cases of CGNB were divided on the basis of the morphologic features of neuroblastic elements into three prognostic subgroups: "Type A Intermixed," having individual microscopic nests of neuroblasts (N = 4, 100% survival); "Type B Intermixed," having microscopic aggregates of multiple neuroblastic nests (N = 6, 67% survival); and "Nodular," having grossly visible nodule(s) of neuroblastic proliferation (N = 8, 0% survival). Survival rates are significantly different for the prognostic subgroups (P less than 0.025). Each prognostic subgroup demonstrated an immunohistochemically distinct pattern of stromal cell composition in the neuroblastic elements: Type A Intermixed had numerous S-100 cells and no FER cells, Type B Intermixed contained many S-100 cells and a moderate number of FER cells, and Nodular had few S-100 cells with many FER cells. The S-100 and FER scores, determined by counting the positive cells through a line sampling method, differed significantly between these prognostic subgroups. Lymphocytic aggregations in tumor tissue evaluated by volumetric assessment with LCA staining, on the other hand, showed no contribution in predicting the outcome of the patients. There was also an inverse relationship between S-100 and FER score, suggesting a relationship between the relative predominance of these stromal cell types, tumor histopathologic features, and the biologic behavior of CGNB.

Cell Aggregation↗
PubMed1990

Noise-induced hearing loss in randomly selected New York dairy farmers.

To understand better the effects of noise levels associated with dairy farming, we randomly selected 49 full-time dairy farmers from an established cohort. Medical and occupational histories were taken and standard audiometric testing was done. Forty-six males (94%) and three females (6%) with a mean age of 43.5 (+/- 13) years and an average of 29.4 (+/- 14) years in farming were tested. Pure Tone Average thresholds (PTA4) at 0.5, 1.0, 2.0, and 3.0 kHz plus High Frequency Average thresholds (HFA3) at 3.0, 4.0, and 6.0 kHz were calculated. Subjects with a loss of greater than or equal to 20 db in either ear were considered abnormal. Eighteen subjects (37%) had abnormal PTA4S and 32 (65%) abnormal HFA3S. The left ear was more severely affected in both groups (p less than or equal to .05, t-test). Significant associations were found between hearing loss and years worked (odds ratio 4.1, r = .53) and age (odds ratio 4.1, r = .59). No association could be found between hearing loss and measles; mumps; previous ear infections; or use of power tools, guns, motorcycles, snowmobiles, or stereo headphones. Our data suggest that among farmers, substantial hearing loss occurs especially in the high-frequency ranges. Presbycusis is an important confounding variable.

Adult↗
PubMed1990

Laryngeal mask airway for caesarean section.

The use of a laryngeal mask airway is described in a patient for emergency Caesarean section, when tracheal intubation was not possible. Provision of the laryngeal mask airway for use in the Maternity Unit is discussed.

Adult↗