Biomedical subjects
Publications and source records attributed to M Reynes.
3 cases of hairy cell leukaemia were studied with ultrastructural immunocytochemical methods using an anti human Ig HRPO-Fab fragment. Ig were detected on the cell surface, in the perinuclear cisterna and endoplasmic reticulum of hairy cells. Evidence of Ig in these sites demonstrates a B-lymphoid differentiation of the leukaemic cells.
Explore the source record for details and available documents.
An ultrastructural cytochemical study of lysosomal acid phosphatase was performed on leukemic cells in a case of chronic lymphocytic leukaemia of T cell origin (T-CLL). The cells showed inclusion bodies known as parallel tubular arrays, which often lay within acid phosphatase-positive, membrane-bound spaces. This suggests their lysosomal location.
Epidermolysis bullosa acquisita (EBA) is a rare non hereditary bullous disease. One case is described with a morphological, and immunocytochemical ultrastructural study. The plain ultrastructural morphology showed a dense amorphous deposit in the superficial dermis, under the basal lamina. Immunofluorescence showed IgG and C3 deposits. The ultrastructural study (direct immunoperoxidase technique using Fab) showed a dense labelling associating : dark dots spaced under the basal lamina and fogging less dense labelling in the clarified superficial dermis. Using the patient's antibodies an indirect reaction on normal human skin performed : the same dotted sub laminal labelling was found but with no dermal diffuse reaction product. The study shows the site and the aspect of the Ig deposit in EBA and indicates that the antibodies demonstrated in some patients are reactive with a constituent of the normal superficial dermis.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Peripheral lymphoid organs have two groups of non lymphoid cells : that of macrophages which belongs to R. van Furth's system of mononuclear phagocytes and the one made of elements for long labelled "reticular cells". The use of electron microscopy has permitted a good understanding of these elements and a recognition of ubiquitary cells (fibroblasts, endothelial cells) and specific cells to the lymph node which seem to play a very important role in the immune reactions (interdigitated cells, reticulum cells, dendritic cells). We describe their light and electron microscopical features and histoenzymatic characters, and present the actual concept of their origin and function. The latest discoveries on their classification are reviewed and have permitted to distinguish three categories of non lymphoid cells : first the group of reticulum cells (reticulum cells and dendritic cells), second that of mononuclear phagocytes (macrophages and interdigitated cells) and third that of endothelial cells. Other elements of questionable existence or insufficiently known do not appear in this classification : dark cells, Steinman and Cohn's dendritic cells and cells containing Birbeck's granules.
Explore the source record for details and available documents.
This work is devoted to the analysis of the nature of lymphoblastic lymphoma/leukemia with convoluted nuclei which were initially described by Barcos and Lukes. Ultrastructural, cytochemical, and immunologic features of tumor cells were investigated in patients chosen according to known morphologic criteria. Through results of the E rosette test, the patients were divided into two groups (E+ and E-). In the E+ group, the predominant features were sex (only men), the mediastinal localization, and the focal positivity of the acid phosphatase reaction. Cytotoxicity tests with rabbit antihuman T-lymphocyte anti-serum confirmed the results of the E rosette test in the 3 patients of the E+ group who were tested and were also positive in 2 patients from the E- group (1 of these 2 patients had the characteristics found in the E+ group and can thus be related to this group; the other patient had none of these characteristics). This raises the question of a leukemia arising from a less differentiated T-cell but this interpretation is limited by the specificity of the anti-T-serum. Ultrastructural study defines more precisely the convoluted aspect but does not at present allow a distinction between the two groups.
The new classifications adopted for lymphoid tumors now include a particular type of proliferative process: immunoblastosarcoma. This tumor is not a rare occurrence in the orbit, as shown by the two cases reported, and possesses sufficiently specific clinical and histologicas characteristics to justify its appearance in a separate class. The cytological and histological characteristics of the immunoblasts from which it develops are described. Tumors of this type were previously mostly included in the group of reticulosarcomas, now become obsolete because of poorly defined characteristics of the reticular cell. The prognosis is poor in spite of the chemotherapeutic and radiotherapeutic treatments proposed.
Two cases are reported of an apparently distinct type of immune disorder. Beginning with mild anaemia and widespread massive lymphadenopathy, the disease progressed to a fatal autoimmune type haemolytic anaemia. Serum investigation showed polyclonal hypergamma-globulinaemia and some autoantibodies. Repeat lymph node biopsies in each case showed hyperplasia within B lymphocyte territory (follicular hyperplasia and polyclonal plasmacytosis with IgG predominance) and atrophy of T dependent areas. Dilatation of lymph sinuses, vascular proliferation, and sclerosis were striking features. This appears to be a new entity, and reasons are given for separating this disease from other pseudotumourous lymph node disorders associated with dysimmunity.
Explore the source record for details and available documents.
A case of Hodgkin's disease is described which developed into a terminal illness characterized by a malignant proliferation of T-cells. The leukemic cells, after optical and ultrastructural analysis, were distinct from those of myelomonocytic, acute lymphoblastic, chronic lymphocytic as well as prolymphocytic leukemia. Their relationship with the T-cell lineage seemed to be confirmed by a highly positive E-rosette test and by cytochemistry which showed focal positivity of acid phosphatase. The importance of this T-cell malignant proliferation is discussed, especially with regard to cellular interactions in Hodgkin's disease.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Bone marrow plays a considerable role in the diagnosis of hairy cell leukemia. The morphological aspects should be well known. One should emphasize the existence of aplastic or hypoplastic forms requiring one to repeat biopsy on the slightest clinical suscpicon. Routine histological techniques are sufficient for the diagnosis. The ultrastructural study also provides valuable histological and cytological evidence.
A description of a non-excretory myeloma located in the orbit. The authors stress the importance of immunofluorescent and ultra-structural studies in the identification of these tumours. They discuss the relationship between multiple myeloma and extra-medullary plasmocytoma and raise the possibility of modes of transmission.
The case was one of a 25-year-old woman with a polycystic subcutaneous nodule in the posterior iliac region, developing three weeks after a series of injections. Histological examination revealed the presence of cysts with a saccular formation filled with endospherules characteristic of the disorder. Myospherulosis is reviewed in the light of this first european case.