Biomedical subjects

M Ribó

Publications and source records attributed to M Ribó.

22 records · Page 2Linked to original sources

PubMed1994

A versatile negative-staining ribonuclease zymogram.

A versatile negative-staining ribonuclease zymogram is described. The method has several advantages as it combines, by means of different staining procedures, high resolving power, sensitivity, and specificity with a rapid, reproducible, and simultaneous analysis of purity of ribonuclease samples on the same polyacrylamide gel. Activity bands can be visualized at any time during the incubation process without staining of the gel. This allows the choice of different staining procedures after incubation. Using poly(C) as substrate less than 1 pg of bovine pancreatic ribonuclease A was detected in less than 2 h after the electrophoretic run. An additional advantage with respect to other methods is that no refrigeration is needed during electrophoresis.

Adenocarcinoma↗

PubMed1994

Heterogeneity in the glycosylation pattern of human pancreatic ribonuclease.

Different molecular forms of ribonuclease were isolated from fresh human pancreas obtained from healthy transplant donors. The purification procedure consists of the preparation of an acetone powder, followed by (NH4)2SO4 precipitation and two chromatography steps (cationic exchange and reversed-phase). Protein bands in gel electrophoresis with RNAase activity were monitored using a negative-staining zymogram technique. Several glycosylated enzyme forms with apparent molecular masses ranging from 14 to 40 kDa were separated. Peptides containing the three Asn-Xaa-Thr/Ser acceptor sites for glycosylation were isolated and analysed. The site with Asn-34 was almost completely glycosylated, while the sites with Asn-76 and Asn-88 had carbohydrate in about half and a minor part of the molecules, respectively. The carbohydrate compositions of the glycopeptides are different from those of the same gene product isolated from human urine. C-Terminal threonine was present in part of the molecules, indicating partial degradation by carboxypeptidase.

Amino Acid Sequence↗

PubMedDate not supplied

[Chronic subdural hematoma simulating a TIA. Implications for the management transient neurological deficit].

Chronic subdural hematoma that generally happens after cranial trauma doesn't have clinical manifestations until days or weeks after the traumatism. Due to the lesions nature, symptoms are almost always progressive and presentation as transient ischemic attacks (TIA) is very uncommon. We describe 2 cases of chronic subdural hematoma that started simulating TIA and we discuss its implications in the management of those patients who had presented transient neurological deficit.

Aged↗

PubMedDate not supplied

[Clinical factors predicting the appearance of vascular events following a transient ischemic attack].

INTRODUCTION: Patients who have suffered a transient ischemic attack (TIA) have a high risk of undergoing a severe vascular event. Most of them do not receive early attention and cannot benefit from an adequate diagnosis and treatment. AIMS: The aim of this study is to identify the clinical factors that predict the appearance of a new vascular event in these patients. PATIENTS AND METHODS: We conducted a prospective study of 104 TIA patients, who had been given attention during the first 24 hours after the onset of symptoms, for a mean follow up time of 12.6 months. RESULTS: The incidence rates of strokes, ischemic heart disease, peripheral arteriopathy and death were 13, 6, 3 and 8%, respectively. Multivariate analysis identified the following aspects as independent predictors of new strokes: an age above 73 odds ratio (OR) 4.46 (CI 95%, 1.15 17.38) and an atherothrombotic aetiology OR 4.36 (CI 95%, 1.4413.18); and episodes of ischemic heart disease, a history of suffering from such heart disease OR 30.65 (CI 95%, 2.94319.17) and taking oral antidiabetic drugs OR 31.23 (CI 95%, 2.65368.74). Leukocytosis OR 11.21 (CI 95%, 1.25100.39) is linked to the deaths caused by vascular disorders, whereas an atherothrombotic aetiology OR 3.83 (CI 95%, 1.31 11.20) and being male OR 3.44 (CI 95%, 1.02 11.60) were seen to be predictors of the appearance of any vascular event. CONCLUSIONS: Our findings showed that the risk of suffering severe vascular events after a TIA becomes higher. There are a number of useful clinical variables (age above 73, atherothrombotic aetiology, being male, taking oral antidiabetic drugs and leukocytosis) for identifying the patients at the highest risk.

Age Factors↗