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M Ristić

Publications and source records attributed to M Ristić.

At least 19 recordsLinked to original sources
PubMed1992

A stereological investigation of rat endocrine pancreas after a long-term low-protein diet.

Stereological analysis of rat endocrine pancreas was carried out after 4 months of low-protein intake. Thirty make Wistar rats, aged 2 months at the beginning of the experiment, were divided into three groups. The control group (NP) received a standard laboratory diet. The experimental group (LP) received a low-protein diet, and the pair-fed animals also received a standard laboratory diet, as the NP rats did, but restricted to the amounts consumed by the LP group. All groups were offered drinking water ad lib. Weibel's multipurpose lattice was used to determine the volume fraction of cells and numerical density of islets in the endocrine pancreas. The results showed that the long-term low-protein diet decreased the volume fraction of B-cells and increased that of A-cells. Numerical density of islets was significantly increased, whereas the absolute number was decreased. The mean number of B-cells per islet was found to be decreased and that of D-cells increased. The mean radius of the islets was decreased, as was the mean surface area.

Animals↗
PubMed1990

[In vitro culture of cells from patients with leukemia].

The development of cytological, cytochemical and immunological investigations of leukemic cells as well as new cytostatic drug combinations have led to great progress in the diagnosis and treatment of acute leukemia (AL). However, a heterogeneity in response to the same kind of therapy has been noted in patients with the same cytological type of the disease. This points out the importance of investigations of leukemic cells in culture which should provide more data on proliferation and reactivity of these cells. The population of tumor cells in heterogeneous in morphological and biochemical properties, in proliferative capacity and other characteristics. In cell culture it is possible to investigate the growth of cells and the effects of various agents on normal and malignant cells. In this study we investigated the growth of cells from bone marrow of patients with acute leukemias and acute phase of chronic leukemias. Cells from 17 patients were studied. The patients were from Internal Clinic A in Belgrade and the investigations were performed before treatment. Bone marrow cells were cultured in vitro on plastics and growth of the adherent cells was followed in the course of 2-3 weeks. The results show that bone marrow cells adhered and proliferated in 12/17 patients, there was low proliferation in 4/17 and none in 1/17. It is shown that in these conditions a successful growth of fibroblast-like shaped cells can be obtained with the bone marrow cells, but it is still not possible to correlate the course of the disease and the proliferation in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)

Humans↗
PubMed1990

[Hereditary factors in the etiology of chronic lymphocytic leukemia].

Leukocyte counts, hemoglobin concentration, PAS positivity index in lymphocytes, leukocyte alkaline phosphatase, karyotype, HLA phenotype as wall as the quality of the cellular and humoral immunity have been studied in 45 patients with chronic lymphocytic leukemia (CLL) and in 103 their closest relatives (sibs, parents). The aim was to detect the possible preleukemic condition in the relatives as a strong propensity towards developing has been previously established in families of patients with CLL. In the CLL patients our studies have confirmed the results of the determination of similar parameters by other authors, namely a higher percentage of PAS+ lymphocytes than normal together with a variety of humoral and cellular immunity disturbances. Regarding the morphological and cytochemical changes in lymphocytes in family members of the CLL patients a higher frequency of PAS+ lymphocytes have been observed: it has been established in over 10 per cent lymphocytes (normally 4-8 per cent) in 31 (30%) examinees and in 14 (13.5%) of these persons the percentage of PAS positivity was equal to that found in CLL patients. Cellular immunity examinations established that in one third of examined E rossete counts counts were lower and in 2 persons the degree of this decrease corresponded to the decrease observed in CLL patients. In one fifth of the examined family members T4:T8 ratio was reduced. In the 13 per cent of the examined M rossete counts were increased, a fact which supports the notion that immunity of B cells exists in relatives of CLL patients. The authors contend that the increased percentage of PAS+ lymphocytes and inadequate functional maturity of the T and B cells in a relatively large number of family members of the CLL patients, intimates that a hereditary disturbance in the lymphocyte make up and their role in the immunity pathways exists and could possibly represent one of the factors implicated in a high frequency of CLL in some families.

Aged↗
PubMed1990

[Morgagni's diaphragmatic hernia--case report].

A sucostosternal hernia (type Morgagni) is a rare diaphragmatic anomaly of congenital origin; in that sense are data that from 100 operated on diaphragmatic hernias, only three belong to this type. Although it is known that 20% of patients with congenital diaphragmatic hernia have other congenital anomalies, a coincidence of a Morgagni's hernia with heart anomalies, in available to use (domestic and foreign) references, there has not yet been reported. Besides a Morgagni's hernia, our patient also had an insufficiency of mitral and tricuspidal valves, atrium septum defect (sec.), pulmonary hypertension and Hiss' right branch block. Surgery was successful: the diaphragmatic defect (size: 7 cm. x 3 cm.) repaired, and the complex heart anomalies corrected in an extracorporeal circulation.

Adult↗
PubMed1989

Acute myelomonoblastic leukemia in a patient with multiple myeloma.

A 40-year-old man who developed acute myelomonoblastic leukemia (M4) after 7 years of treatment for multiple myeloma with the alkylating agent melphalan and steroids is presented. Leukemia was treated with courses of adriblastin, cytosine arabinoside, and thioguanin (DAT protocol), with a 8 months' survival.

Adult↗
PubMed1989

Nutritional status and plasma lipids in diabetic subjects.

The aim of the present work was to determine and analyse the nutritional status and plasma lipid levels in diabetic and control subjects dependent on the type of lipidemia. The investigations involved four groups: controls with normal lipidemia, diabetic (NIDDM) subjects with normal lipidemia, persons with hyperlipidemia type IV, and without diabetes mellitus, and diabetic subjects with hyperlipidemia type IV. The values for the concentrations of plasma triacylglycerol, cholesterol, phospholipids and HDL-phospholipids were significantly higher in both diabetic groups than in controls. The values for the relative amount and concentration of HDL-cholesterol were significantly lower in diabetics than in the control groups.

Body Weight↗