PubMed Health⌕ Search

Biomedical subjects

M Rodrigues

Publications and source records attributed to M Rodrigues.

121 records · Page 7Linked to original sources
PubMedDate not supplied

[Treatment of unspecific ulcerative rectocolitis in a child with enemas containing butyrate. Case report].

OBJECTIVE: To report a promising therapy to ulcerative colitis in children. METHODS: We report a case of ulcerative colitis in 4 years old female, who had this diagnosis since she was 1 year old. The treatment of RCU1 had an unsatisfactory response to conventional therapies (rectal and oral corticosteroids and immunosuppressive agent). RESULTS: She showed improvement, using enemas containing butyrate, in clinical, endoscopic and histological parameters. CONCLUSIONS: Although the role of butyrate in the treatment of ulcerative colitis still remains to be determined, we find effective response in this case. Despite evidences that butyrate may be beneficial and safety in treating refractory distal ulcerative colitis, results of controlled trials, specially in children will be necessary to prove the efficacy of this therapy.

Administration, Oral↗
PubMedDate not supplied

[Phlebotomines collected in remaining forests and wild animal shelters from the zoological garden of the Maringa urban area, southern Brazil. Preliminary study].

The present study was undertaken after the detection of one case of cutaneous leishmaniasis with presumed infection in one of the three remaining wooded areas in the urban area of the city of Maringá, Southern Brazil; also in view of the lack of knowledge about sand flies and their behavior. From June to September, 1995, sand flies were caught with Falcão traps during the night in the remaining wooded areas (Parque do Ingá, Bosque Dois and Horto Florestal). A total of 2,907 sand flies were caught in Parque do Ingá; 1,723 of them were aught in forest traps and 1,184 in wild animal shelter traps at the zoo. The results show that Lutzomyia whitmani is better adapted to the three areas under study and that it frequently occurs in wild animal shelters within the urban perimeter of Maringá.

Animals↗
PubMedDate not supplied

[Immunohistological study of T-lymphocytes (total and helper) and B-lymphocytes of the colonic mucosa in children with Crohn's disease and non-specific ulcerative colitis].

For one to understand better the immunological mechanism in the colonic mucosae of inflammatory bowel disease, 19 children with ulcerative colitis and 12 children with Crohn's disease were studied. The inflammatory cells identified by the immunoperoxidase technique and specific markers for total T lymphocytes (CD3), helper T lymphocytes (CD4) and B lymphocytes (CD20) in differents evolutionary periods of the disease were analysed. PI (before treatment), PII (until two years of treatment) and PIII (with more than two years of treatment). In general, all marker studied in this research, presented higher averages if comparing to the control group with different degrees of significance in the different studied periods. The marker CD3, of the total T lymphocyte, increased in the epithelial (superficial and glandular) and in the lamina propria of the involved mucosae in relation to the controlled ones, was diffusely distributed over the mucosae, concentrating itself around the microgranulomas and the nodules lymphoids. In Crohn's disease, PI, its average differed significantly from the controlled ones. Similar behave was seen in the marker CD20 of the lymphocytes in the involved mucosae, whole averages were superior in relation to the controlled ones; thought, only in the PI of the Crohn's disease this difference had been significant. The immunochromatic cells CD3 distributed themselves diffusely over the involved mucosae, concentrating themselves in the interior of the lymphoides nodules and in the injured areas, near the macrophages. The marker CD4, of helper T lymphocyte also presented in the involved mucosae high averages in relation to the controlled mucosae, but without estatistic significance. The CD4 cells distributed themselves diffusely over the whole thickness of the involved mucosae, concentrating themselves around the lymphoids nodules and microgranulomas. These results confirm the participation of these elements in the pathogenesis of inflammatory bowel disease, but did not permit the establishment of any discriminatory criteria between ulcerative colitis and Crohn's disease. The results of positive correlation between the increased of CD4 and CD20 in ulcerative colitis and CD3 and CD20 in Crohn's disease show clearly the immunoregulation action of the CD4+ cells over clonal expansion and the differenciation of the B lymphocytes from plasmocytes and the IgG syntese.

Adolescent↗
PubMedDate not supplied

[Immunohistochemical study of colonic mucosa macrophages in children with Crohn's disease and ulcerative colitis].

In this research the histological characteristics of the macrophages on the colonic mucosa in Crohn's disease and ulcerative colitis were quantified and analysed. Twelve Crohn's disease, 19 ulcerative colitis and 10 specimen of the rectal mucosa, representing the control group according to the followed model, were studied: I period (PI) = pre-treatment, II period (PII) = up two years of evolution and III period (PIII) = more than two years of evolution. The macrophages were identified in a colonic mucosa by the monoclonal CD68 through the immunoperoxidase method. The macrophages quantification was done by chromatic computer images analysis, that express the area (mm2) used by the CD68 positive cells, in percentage. The percentage of the area used by the macrophages was increased in both diseases, in all the studied periods, when compared with the control group, but without statistic significance. The macrophages' distribution inside the control group mucosa was subepithelial, while in the illness group, it reached all the mucosa that was concentrated on the basis of ulcers and all long the fissures. On the Crohn's disease the CD68 positive cells facilited the identification of the microgranulomas, sometimes unnoticed in the hematoxiline-eosine. Although there was no difference between patients and control group in the macrophages area, the difference in the distribution could suggest the macrophages' participation on the injure in both diseases although they do not permit a differential diagnosis because of the variety of the values. The CD68 did not identify the different functional status of the macrophages, but their position in the mucosa suggest that, in terms of fissures and ulcers, their mainly function should be the phagocitosis and in the other cases, they have been the cells that should show the antigens and that recruit the other inflammatory cells.

Adolescent↗
PubMedDate not supplied

Spontaneous resolution of diffuse alveolar septal amyloidosis.

Amyloidosis is a generic term for a heterogeneous group of disorders associated with deposition of protein in an abnormal fibrillar form. Amyloidosis can be hereditary or acquired, localised or systemic and potentially lethal or merely an incidental finding. We report a case of a male who was totally asymptomatic and was incidentally detected to have lung amyloidosis. He was not given any treatment but kept under observation., His chest radiograph (CXR) and high resolution computed tomograph (HRCT) of the chest cleared spontaneously after one year and the patient continues to remain asymptomatic even after two years.

Adult↗
PubMedDate not supplied

[Pneumopyopericardium].

Pneumopyopericardium is a rare disease. The most common causes seems to be ulceration or carcinoma in the lower esophagus or upper stomach. We report a case of pneumopiopericardium secondary to a pneumonia in a 60 years-old-woman after the first course of chemotherapy for acute myeloid leukemia. We use this case as a basis for a review of the literature on similar cases. This syndrome can be recognised promptly because of its characteristic physical findings and radiographic features.

Female↗
PubMedDate not supplied

[Pattern of intrauterine growth and Doppler flowmetry].

The aim of this study was to evaluate the accuracy of ultrasound biometric and hemodynamic fetal parameters for the prenatal diagnosis of intrauterine retardation in a sample of 438 pregnant women who had their first visit before the 16th week of gestation at the outpatient clinic of the Department of Obstetrics and Gynecology, S. Joào Hospital. The results are presented for 427 mother-infant pairs corresponding to singleton pregnancies uncomplicated by congenital malformations. The prevalence of SGA was 4.9%, 86.2% for AGA and 8.9% for LGA. A relatively low sensitivity and high specificity were calculated for the ultrasound parameters evaluated, as observed in previous studies performed in other populations. Mean values for the differences of umbilical vessels resistance index measured at 28-32 and 35-37 gestation weeks were significantly lower in SGA newborns, probably reflecting a sustained compromise of vascular compliance (umbilical/placental). The observed lack of agreement between the pre and the postnatal diagnosis of SGA emphasises the need to define local reference birthweight curves or the careful adoption of European standards obtained in populations identical to our.

Adult↗
PubMedDate not supplied

Regional diastolic function in hypertrophic cardiomyopathy: a tissue Doppler echocardiographic study.

BACKGROUND: Tissue Doppler echocardiography (TDE) is a recent ultrasonic technique. One of its potential clinical uses is the assessment of regional diastolic function. OBJECTIVES: To compare left ventricular global diastolic function with interventricular septum regional diastolic function in patients with hypertrophic cardiomyopathy with different levels of global diastolic dysfunction. METHODOLOGY: 10 normal subjects (group 1), eight hypertrophic cardiomyopathy patients with abnormal relaxation (group 2) and 12 patients with hypertrophic cardiomyopathy with restrictive pattern (group 3) on the mitral inflow profile (pulsed wave Doppler) were studied. Each patient performed an M-mode color; TDE of the basal septum in the four chamber view. Velocities during the rapid filling phase (RF), and during atrial contraction (AC) were measured and RF/AC ratio calculated in each patient. RESULTS: While in normal subjects (group 1) the RF/AC ratio was always higher than 1, in patients with hypertrophic cardiomyopathy patients with different levels of global diastolic dysfunction (groups 2 and 3), the RF/AC ratio was always lower than 1 (velocities during atrial contraction always higher than during rapid filling), despite the level of global diastolic dysfunction. CONCLUSIONS: A ratio between myocardial velocities during the rapid filling phase and during atrial contraction lower than one (RF/AC < 1) is a tissue Doppler echocardiography index of regional diastolic dysfunction, independently of the severity of global diastolic dysfunction.

Adult↗
PubMedDate not supplied

Technetium-99m-tetrofosmin: a new agent for melanoma imaging?

The aim of our study was to examine whether technetium-99m 1,2-bis[bis(2-ethoxyethyl) phosphino]ethane (tetrofosmin) a lipophilic, cationic tracer which was first developed for myocardial perfusion imaging, could be a new radiopharmaceutical for melanoma imaging. We therefore used two human cell lines, SK-MEL 28 and 5i8 A2 (n = 6, cell concentration 106/ml, incubation at 22 degrees C and 37 degrees C, 50-100 approximately lCi/ml Tc-99m-tetrofosmin, time of incubation 10-180 minutes). The cellular uptake by both cell lines was determined. In contrast to another non- melanoma tumor cell line MCF-7 (a human adenocarcinoma breast cancer) which reached steadystate almost immediately (within 10 minutes), the cellular uptake of SK-MEL-28 increased after 60 minutes and showed a very high uptake (> 10%) after 120 minutes and decreased after 180 minutes (6-8%), while the uptake in 518 A2 cells was about 5% after 90 minutes. Our data show that Tc-99m-tetrofosmin could be a promising agent for melanoma imaging.

Biological Transport↗
PubMedDate not supplied

[Immunohistochemical study of HLA-DR expression in superficial epithelium and in lamina propria of colonic mucosa in children with Crohn's disease and nonspecific ulcerative colitis].

Some researches have stressed the importance of expression HLA-DR on the superficial epithelium and on the lamina propria of the colonic mucosa of patients with ulcerative colitis and Crohn disease. In this research the histological characteristics of the expression HLA-DR on the resident mucosa of both these illness were quantified and analysed. Twelve Crohn disease, 19 ulcerative colitis and 10 specimen of the rectal mucosa, representing the control group according to the following model were studied: I period = pre-treatment, II period = up two years of evolution and III period = more than two years of treatment. The expression HLA-DR was identified by the monoclonal HLA-DR (DAKO) in the resident mucosa specimen by the immunoperoxidase method. The quantification of the expression HLA-DR on the lamina propria was done through the chromatic computer images analysis that express the area (micron2) used by the positive HLA-DR cells and the superficial epithelium in percentage by a semi quantifier evaluation. On the lamina propria of the illness mucosa, the expression HLA-DR was increased in all the studied periods when compared with the control group, but without statistics differences. The distribution of the expression HLA-DR on the lamina propria was done to the corresponding localization to the macrophages and to the B lymphocytes. The superficial colonial epithelium of the ulcerative colitis and Crohn disease patients was in 86.95% and in 80.96% respectively HLA-DR positive in the three periods. The superficial epithelium of all the rectal mucosa of the control group did not show the HLA-DR expression. The presence of the HLA-DR expression on the superficial colonial epithelium of the ulcerative colitis and Crohn disease patients and its absence in the control group was a very important found in this research. It is a consense among several authors that the HLA-DR expression represents a intense local action of the inductorious cytokines of this expression in the colonic mucosa of the ulcerative colitis and Crohn disease patients, representing its activation in a answer to the invasor antigen in the intestinal mucosa. The study of the HLA-DR expression in the lamina propria was not conclusive, because the result did not represent a significative difference in relation with the control.

Adolescent↗