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M Rogosa

Publications and source records attributed to M Rogosa.

9 recordsLinked to original sources

Method for coding data on protozoan strains for computers.

Traditionally, observations on the nature of protozoa have been published in periodicals or books, or remain buried in research notebooks. The retrieval and processing of information on a particular species or strain are dependent solely upon individual investigators. Although various modern methods have been applied to the study of protozoa, no attempt has been made to develop a system with which information on protozoan strains can be stored, retrieved easily, and processed for various analyses by computer technology. Based upon an existing system for encoding data on bacterial strains, a complementary system applicable to protozoan strains was developed and is described herein.

Animals↗

Acidaminococcus gen. n., Acidaminococcus fermentans sp. n., anaerobic gram-negative diplococci using amino acids as the sole energy source for growth.

Acidaminococcus gen. n. and the type species Acidaminococcus fermentans sp. n. were described. Amino acids, of which glutamic acid is the most important, could serve as the sole energy source for growth. Acetic and butyric acids and CO(2) were produced; propionic acid and hydrogen were not produced. Amino acid media supporting growth and the amino acid and vitamin requirements were described. Glucose was frequently not fermented or was weakly catabolized. Derivative products from glucose autoclaved in media, but not glucose itself, stimulated or were required for growth in amino acid media. A wide range of polyols and carbohydrates were not attacked. Lactate, fumarate, malate, succinate, citrate, and pyruvate were not used as energy sources for growth. Pyruvate completely suppressed growth. Cytochrome oxidase and benzidine reactions were negative; catalase, indole, acetyl methyl carbinol, and H(2)S were not produced; nitrate and sulfonthalein indicators were not reduced; ammonia was produced; gelatin liquefaction was negative or slow and partial; vancomycin (7.5 mug/ml) was resisted. Acidaminococcus was different from Veillonella in morphology, serology, nutrition, utilization of substrates, and accumulation of products in media supporting growth; Acidaminococcus resembled Peptococcus in utilization of glutamic acid and accumulation of similar products, but the two genera differed in morphology, gram reaction, serology, guanine plus cytosine content of deoxyribonucleic acid, and nutrition.

Aldehydes↗

Direct quantitative gas chromatographic separation of C2-C6 fatty acids, methanol, and ethyl alcohol in aqueous microbial fermentation media.

A method is described for the direct quantitative gas chromatographic separation of C(2)-C(6) lower fatty acid homologues, methanol, and ethyl alcohol in aqueous microbial fermentation media. A hydrogen flame detector and a single-phase solid column packing, comprising beads of a polyaromatic resin (polystyrene cross-linked with divinyl benzene), were employed. Direct injections of 1 to 10 muliters of aqueous culture supernatant fluids were made. Quantitative recoveries of C(2)-C(6) acids added to culture supernatant fluids were obtained.

Acetates↗

Truncated Glycolytic System in Veillonella.

Rogosa, M., (National Institutes of Health, Bethesda, Md.), M. I. Krichevsky, and F. S. Bishop. Truncated glycolytic system in Veillonella. J. Bacteriol. 90:164-171. 1965.-Intact Veillonella cells do not utilize carbohydrates for growth, nor are carbohydrates fermented. In cell extracts, there is no detectable glucokinase or fructokinase. Cell extracts do not degrade glucose or fructose unless supplemented with yeast hexokinase. Under these conditions, triose phosphates are formed in the presence of a hydrazine trap. When glucose-C(14) plus added hexokinase or fructose-1,6-diphosphate-C(14) was incubated with cell extracts, the production of CO(2), acetate, pyruvate, propionate, and lactate was detected. It is concluded that, except for a hexokinase, all the activities required for a glycolytic system are present.

Journal Article↗

The Genus Veillonella IV. Serological Groupings, and Genus and Species Emendations.

Rogosa, M. (National Institute of Dental Research, Bethesda, Md.). The genus Veillonella. IV. Serological groupings, and genus and species emendations. J. Bacteriol. 90:704-709. 1965.-Seven serological groups of Veillonella were found. The generic and species descriptions are enlarged and corrected. The type species is Veillonella parvula (Veillon and Zuber) Prévot, 1933. The proposed species, subspecies (infraspecific serogroups), and American Type Culture Collection (ATCC) type strains are: Veillonella parvula subsp. parvula subsp. nov. (antigenic group VI), ATCC 10790; Veillonella parvula subsp. rodentium subsp. nov. (antigenic group II), ATCC 17743; Veillonella parvula subsp. atypica subsp. nov. (antigenic group V), ATCC 17744; Veillonella alcalescens subsp. alcalescens subsp. nov. (antigenic group IV), ATCC 17745; Veillonella alcalescens subsp. ratti subsp. nov. (antigenic group III), ATCC 17746; Veillonella alcalescens subsp. criceti subsp. nov. (antigenic group I), ATCC 17747; and Veillonella alcalescens subsp. dispar subsp. nov. (antigenic group VII), ATCC 17748. The following species listed in Bergey's Manual (7th ed.) are rejected as members of the genus: V. discoides, V. reniformis, V. orbiculus, and V. vulvovaginitidis.

Journal Article↗