Biomedical subjects

M Rogowski

Publications and source records attributed to M Rogowski.

At least 19 recordsLinked to original sources

PubMed2005

Human red blood cells' physiological water exchange with the plasma.

In the present paper, fundamental issues related to the mechanisms of human red blood cells' physiological water exchange with the plasma (for the stationary conditions) have been discussed. It has been demonstrated, on the basis of mechanistic transport equations for membrane transport that red blood cells are capable of exchanging considerable amounts of water with the plasma. Water absorption is osmosis-driven, and its removal occurs according to the hydromechanics principle, i.e. is driven by the turgor pressure of red blood cells. This newly-acquired knowledge of these issues may appear highly useful for clinical diagnosis of blood diseases and blood circulation failures.

Animals↗

PubMed2004

Loss of heterozygosity in laryngeal cancer.

PURPOSE: Head and neck cancers account for about 6% of all human cancers. Molecular changes leading to the disease development and progression still remain not fully explained. Examination of loss of heterozygosity (allelic loss, LOH) using the specific microsatellite markers is a method of choice in assessing tumour suppressor genes (TSGs) localisation in human genome. MATERIAL AND METHODS: The study was performed in a group of 46 male patients, aged 42-77 years. Forty three patients underwent total laryngectomy with lymph nodectomy, two patients--chordectomy and one patient--partial laryngectomy. Tumour tissue specimens and reference peripheral blood samples were obtained during surgical resections. Standard methods were used for DNA isolation. Fluorescent multiplex PCR was used to amplify microsatellite loci included in commercially available human identification kits. RESULTS: LOH was found at the following loci: BAT26, D3S1358, FGA, CSF1PO, D5S818, D8S1179, VWA, D13S317, D18S51. The highest LOH frequency was found in the tumor samples where the neighbouring cervical lymph nodes were affected but the incidence of LOH at BAT26 was statistically insignificant (p = 0.07). CONCLUSIONS: High incidence of LOH is considered an unfavourable prognostic factor accompanying an aggressive nature of the tumour and indicating an involvement of certain genome regions in cancerogenesis. In head and neck cancers LOH was found on the following chromosomes: 3p, 5q, 8p, 9p, 9q, 11q, 17p, 17q, 18p, 18q.

Adult↗

PubMed2003

The evaluation of voice in elderly patients.

Results of voice analysis in 96 elderly patients are presented. The objective of the study was to evaluate dysphonia and its morphologic conditions. Videolaryngostroboscopic (VLSS) examination distinguished between oedematic and atrophic form of senile dysphonia. Morphologic evaluation of the vocal fold mucosa with the use of light microscopy (LM) and transmission electron microscopy (TEM) confirmed the clinical diagnosis. Evaluation of aerodynamic factors of the larynx function, particularly MPT and VVI, suggested hyperfunctional and hypofunctional modes of the voice formation in patients with the larynx oedema and in patients with atrophic changes, respectively.

Aged↗

PubMed2001

Experiences in the treatment of patients with tinnitus and/or hyperacusis using the habituation method.

The purpose of this study was to summarize the results achieved by patients with tinnitus and/or hyperacusis during the Tinnitus Retraining Therapy. One hundred cases, out of 516 patients registered until January 1999 at the Tinnitus and Hyperacusis Management Clinic, Warsaw, Poland, were examined. The patients have been treated for at least 10 months but not longer than 1 year. A questionnaire specially prepared for this program was used to assess the results. In the group of 100 cases, the results after a minimum of 10 months' therapy are different in each category but they show a significant improvement in about 70% of cases.

Female↗

PubMed2001

Effects of tinnitus retraining therapy (TRT) for patients with tinnitus and subjective hearing loss versus tinnitus only.

The patients with tinnitus and/or hyperacusis undergoing an 18-24 month period of TRT are divided into five categories of treatment. Different types of counselling and sound therapy are used in each category. Selection of patients into a specific category depends on such factors as: hyperacusis, subjective hearing loss and long-lasting effect of noise on tinnitus. The 108 cases were evaluated After 1 year of treatment. The results of therapy of 40 patients with tinnitus and subjective hearing loss (category II) were compared with the results of therapy of patients with tinnitus only (categories 0 and I). A special questionnaire, answered before and during the treatment, was used to assess the results. Our data indicate significant improvement in about 70% of patients with tinnitus only and in about 90% of patients with tinnitus and subjective hearing loss after one year of therapy.

Female↗

PubMed2001

[Preliminary assessment of the role of IL-5 in the pathogenesis of nasal polyps].

Preliminary results of IL-5 levels in homogenates derived from nasal polyps tissue are presented. The IL-5 concentration was assessed by immunoenzymatic method (ELISA, R&D, Systems Minneapolis). 26 patients at age 21-60 years were examined. IL-5 cevels were higher in polips with allergic and were examined. IL-5 levels were higher in polips with allergic and aspirin idiocracy background compared with inflammatory polips. In addition, IL-5 levels were increased in recurrent polips.

Adult↗

PubMed2001

Assessment of the ability of mononuclear blood cells to produce IFN-gamma in patients with laryngeal cancer.

The ability of mononuclear blood cells (PBMC), derived from patients with cancer of the larynx, to produce IFN-gamma in vitro was assessed in this paper. Thirty patients (27 male, 3 female) were qualified to the study. Their mean age was 65 (range: 41 to 78 years), tumour sizes found in the group were from T2 to T4, levels of pathologic malignancy G2 or G3. The percentage rates different blood cells phenotypes (CD3+, CD3+ HLADR+, CD4+, CD8+, CD14+ HLADR+, CD19+, CD56+) were evaluated by means of flow cytometry (Coulter EPICS XL). The control group consisted of 20 healthy blood donors. PBMC were derived by centrifugation of heparinized venous blood on Lymphoprep gradient according to Boyum method. Double cell cultures were performed for 24 hours with antibody anti-CD3 or recombinant interleukins 12 (rhIL-12) or 18 (rhIL-18). The statistic analysis was based on Student t and Mann-Whitney's test with significancy at p<0.05. A significant decrease in the production of IFN-gamma by PBMC in patients with laryngeal cancer after stimulation with antiCD3 (p=0.018), rhIL-12 (p=0.027), rhIL-18 (p=0.016) was found in comparison with the controls. The results suggest a decreased production of IFN-gamma in patients with cancer of the larynx.

Adjuvants, Immunologic↗

PubMed2001

Voice creating aspect after horizontal laryngectomy and chordectomy in patients with carcinoma of the larynx.

Voice quality was assessed in 55 patients with the laryngeal carcinoma. A quality of voice was examined in 18 patients before and after chordectomy and in 37 patients before and after supraglottic surgery. Subjective and objective spectrography methods were applied to evaluate dysphony. The larynx was examined by indirect larngoscopy and videolaryngostroboscopy (VLSS). Significant voice pathology was found in patients before surgery when compared with the normal group. A change of voice colour was found, which was manifested in spectrography by decreased in formant levels, especially F3 and F4 in patients after supraglottic surgery. Dysphagia and longer tracheostomy were temporary complications after the surgery and resulted in further phoniatric rehabilitation. Early phoniatric rehabilitation after chordectomy helped to achieve subjective and objective improvement of voice quality in patients after surgery. Good voice quality in patients after chordectomy is due to preserved structure and increased levels F1, F2, F3, and F4 formants in spectrography.

Aged↗

PubMed2001

Voice analysis after the partial laryngectomy in patients with the larynx carcinoma.

The paper presents 170 cases of patients with the larynx carcinoma after the partial laryngectomy. According to the procedure which we used, vertical, horizontal and subtotal supraglottis laryngectomies were distinguished. Voice pattern was analysed with the use of subjective and objective spectrography. The lowest degree of dysphonia was found in patients after the horizontal laryngectomy, while the highest degree dysphonia in those after subtotal supraglottis laryngectomy.

Aged↗

PubMed2001

Evaluation of the voice function after the supraglottis subtotal laryngectomy.

Voice quality was analysed in 39 patients with the larynx carcinoma after the supraglottis subtotal laryngectomy. Voice pattern was analysed with the use of subjective and objective spectrography before and after the surgery. A deteriorated voice quality was found after the surgery. The spectrographic examination revealed decreased frequency levels of the formants F3 and F4 and the presence of a noise component generated in the glottis area.

Aged↗

PubMed2000

Organization of tinnitus management in Poland.

Spontaneous idiopathic tinnitus is a significant interdisciplinary therapeutic problem. Based on different programs of tinnitus treatment, we organized a team of physicians, psychologists and engineers in order to establish the needs for the first Tinnitus Clinic in Poland. At the same time, together with number of clinical centres, scientific societies and non-governmental organizations, we carried out training and an information campaign throughout the country and initiated the first epidemiological studies survey about tinnitus in Poland. Over a period of 2 years we have provided care for almost 1000 patients, including them in a IS 24-month therapeutic program at the clinic. As a method of choice, Tinnitus Retraining Therapy (TRT) based on a neurophysiological model of tinnitus origin is used. We present here epidemiological data on tinnitus and hyperacusis in Poland.

Adult↗

PubMed2000

[Transient evoked otoacoustic emissions (TEOAE) in tinnitus patients treated with xylocaine].

The group of patients suffering from tinnitus was treated with intravenous Xylocaine. Ten consecutive doses of Xylocaine (2 mg/kg b.m.) were administered during a period of ten days. Changes of amplitudes of TEOAE in relation to tinnitus suppression was assessed. In 91.5% of cases we observed correlation between amplitude changes and complaint abatement. Both amplitude changes and the time of tinnitus suppression increased after ten days of treatment in comparison with single dose of Xylocaine. The differences of amplitude variations between tinnitus and non tinnitus ear group was statistically significant (Wilcoxon test p < 0.05). The treatment with Xylocaine was considered to be the beginning of long-term procedure aimed at causing habituation of tinnitus in patients conscience (TRT).

Adult↗

PubMed1999

Observation of through-hydrogen-bond 2hJHC' in a perdeuterated protein.

It is demonstrated that J connectivity between amide protons and hydrogen-bond-accepting carbonyl carbons can be observed in perdeuterated human ubiquitin. A selective pulse scheme is used to detect these small 2hJHC' interactions in the presence of the much larger through-covalent-bond 2JHC' and 3JHC' couplings. The ratio of the observed through-H-bond correlation intensity and the 2JHC' connectivity observed in a reference spectrum indicates 2hJHC' values of ca. 0.4-0.6 Hz, which are only slightly smaller than the corresponding 3hJNC' values. However, for technical reasons, 2hJHC' couplings are more difficult to measure than 3hJNC'.

Amides↗

PubMed1999

[Low-power laser in the treatment of tinnitus--a placebo-controlled study].

The present study was performed on 32 patients in order to investigate the effect of low-power laser on their tinnitus. The patients were divided into two groups. One group received laser therapy and the other was given a placebo procedure. The effect was evaluated by the use of visual analogue scales. Within the patient group transiently evoked otoacoustic emissions (TEOAE) were measured before, during and after therapy. No significant difference between laser and placebo was found in annoyance or loudness of the tinnitus and in changes of TEOAE amplitude. These results indicate that there is no relationship between the effect of low-power laser and changes in cochlear micromechanics.

Adult↗

PubMed1998

[Lasting hearing loss in the course of neuro-borreliosis].

Lyme borreliosis is spirochetal disease that frequently affects the nervous system months or years after infection giving rise to a varied clinical picture named neuro-borreliosis. We report a case of 47 year old female with progressing hearing loss, tinnitus, paraparesis and ataxia. The disease was beginning six month earlier by weakness of the lower limbs associated with hearing loss. The patient did not remember to be exposed to ticks and did not recall the presence of erythema migrans, arthritis or other systemic signs. CSF was with mononuclear pleocytosis and protein concentration over 600 mg%. Firstly patient was unsuccessfully treated like encephalomeningitis with tbc etiology. Next cerebrospinal fluid analysis showed presence of antibodies against Borrelia burgdorferi in IgG and IgM class. IgG antibodies in serum were founded. Audiometry electric responses from brain stem showed sensorineural hearing loss. Therapy with ceftriaxone was successful. Because negative tick bile history and many signs from different parts of CNS, relationship of this spirochetal infection and severe otolaryngological and neurological disease was firstly difficult to recognition.

Antigens, Bacterial↗

PubMed1998

[Evoked otoacoustic emissions in the neonatal hearing screening].

Early identification of hearing impairment in children is a major problem for clinical research and development. Transient click evoked otoacoustic emissions (TEOAE) have been shown to be a useful test of hearing impairment, especially when used for infant screening. The purpose of this study was evaluation of transiently evoked otoacoustic emissions (TEOAE) for the two-stage screening of infant hearing. The first stage was measurement of otoacoustic emissions. TEOAE were performed in 200 infants. 17.5% of the children exhibited risk factors for hearing impairment. Those failing the second stage proceeded to auditory brain-stem response (ABR). The absence of TEOAE in both ears in 2 children was detected by ABR. The data obtained show that two stage newborn hearing screening is practicable, effective, low-cost and safe.

Acoustic Stimulation↗

PubMed1995

[Morphologic study of the guinea pig cochlea after insertion of cochlear prosthesis electrode materials].

Experience has shown that a special cochlear-implant procedure, the so-called "soft surgery technique", may help to retain the remaining function of the inner ear. The morphological changes in the organ of Corti after an atraumatic implant procedure were examined using an animal model. The insertion was facilitated by coating the electrode with Healon. Scanning electron microscopy of the organ of Corti revealed only minor changes in the inner and outer hair cells. In all cases connective tissue was found sealing the implant as a reaction of the cochlea to the intracochlear implant.

Animals↗

PubMed1995

[Transitory evoked otoacoustic emissions in children with impaired middle ear ventilation].

Transient evoked otoacoustic emission (TEOAE) has proven to be a useful clinical screening test for auditory dysfunction. Problems concerning the evaluation are mainly due to irregularities of the physiological middle ear functions. In this study different degrees of middle ear alterations were quantified by means of tympanometry, otoscopy and pure-tone audiometry and then related to the results of TEOAE registration which were classified by the coefficient of the cross correlation and the frequency response spectrum. Data were obtained from 82 ears of 41 children between the age of 3 and 7 years. When children displayed a tympanogram with no observable peak, a successful registration of TEOAE was possible in only 10.5% (n = 19) of the cases. Such results are related to serous or mucoid effusions which probably prevent a transmission of the emission from the cochlea to the meatus. In analysing the related between conductive losses in pure-tone audiometry and TEOAE it was demonstrated that no TEOAE was could be recorded in ears with a conductive loss above 20 dB HL. This study emphasizes the need to reexamine children who do not show positive emissions connected with middle ear imbalances. Only by retesting these children is it possible to determine whether or not cochlear lesions or middle ear problems prevent a positive TEOAE registration.

Acoustic Impedance Tests↗