Biomedical subjects

M Roldán-Pallarés

Publications and source records attributed to M Roldán-Pallarés.

3 recordsLinked to original sources

PubMed2001

Localisation of endothelin-1 mRNA expression and immunoreactivity in the retina and optic nerve from human and porcine eye. Evidence for endothelin-1 expression in astrocytes.

We have investigated the localisation of endothelin-1 (ET-1) mRNA and ET-1-like immunoreactivity in retina and anterior portion of optic nerve from human and porcine eyes. In situ hybridisation method revealed expression of ET-1 mRNA mainly in the innermost layers of the retinas, in the retinal pigment epithelium cells as well as in the astrocytes of the optic nerve. Immunohistochemical studies showed that ET-1-like immunoreactivity appeared in the same regions where ET-1 mRNA was expressed as well as in the inner nuclear layer and in the inner segments of photoreceptors. In the nerve fibre and ganglion cell layers, astrocytes expressed both glial fibrillary acidic protein and ET-1 proteins suggesting that these cells may secrete ET-1. Expression of ETA and ETB receptors in human retina were demonstrated by reverse transcription-polymerase chain reaction. Our results demonstrated expression of ET-1 in glial, neural and vascular components of retina and optic nerve from human and porcine eyes.

Aged↗

PubMed1999

Long-term complications of silicone and hydrogel explants in retinal reattachment surgery.

OBJECTIVE: To compare long-term complications of silicone sponge, silicone rubber, and MIRAgel used as episcleral buckling elements. METHODS: Medical reports were reviewed of 805 patients with cryotherapy and episcleral buckle for rhegmatogenous retinal detachment who were operated on by 1 of us (M.R.-P.) between March 1984 and December 1997. Average follow-up was 76 months. Symptoms and signs of infection or rejection were considered. Care was taken in buckling element removal, considering the material used for scleral buckling (detailed operative note), duration of the buckle, cause of removal, and culture of the removed element. RESULTS: A total of 757 patients were included in the study. Removal of the implant was necessary in 10 patients (1.3%). Silicone sponge (3 [9%] of 32 patients) was more frequently removed than was silicone rubber (2 [0.6%] of 360 patients) or MIRAgel (5 [1.3%] of 386 patients). Silicone sponge needed to be removed a short time after surgery, showing symptoms of acute infection and positive cultures. Silicone rubber was removed 1 year after surgery with symptoms of chronic infection and positive cultures, and MIRAgel implants were removed after long-term follow-up (7-10 years), showing positive cultures in only 20%. CONCLUSION: Periodic long-term follow-up previously recommended for use of other materials also must be recommended for MIRAgel use because of long-term alterations in its chemical composition and eventual swelling of material.

Adolescent↗

PubMed1999

Laser interferometry: preoperative functional predictive value in retinal detachment.

PURPOSE: To determine the functional predictive value of laser interferometry (LI) in retinal detachment (RD). METHODS: Laser interferometry with test field diameters of 3 degrees and 8 degrees, both with and without light, was tested preoperatively in 42 eyes with rhegmatogenous RD that were candidates for scleral buckling. Snellen visual acuity (VA) and LI (four test fields) were tested preoperatively and postoperatively (1 week, and 1, 3, and 7 months). Sensitivity and specificity of LI to predict postoperative (7 months after surgery) VA improvement was calculated. Chi-square and Spearman correlation tests were used for statistical analysis. RESULTS: Statistically significant Snellen VA improvement (P = 0.02) was first found 7 months after surgery. Laser interferometry at 3 degrees without light showed statistically significant improvement 3 months after surgery (P = 0.05). Laser interferometry at 3 degrees without light showed the highest sensitivity (93%) and specificity (87%) values. The best correlation between preoperative LI and postoperative Snellen VA (7 months after surgery) was also found with 3 degrees LI without light (r = 0.71, P = 0.002). CONCLUSION: Laser interferometry (3 degrees without light) was useful in predicting VA improvement in cases with RD preoperatively and postoperatively. Laser interferometry at 3 degrees without light showed the best correlation with postoperative Snellen VA.

Adult↗