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Biomedical subjects

M Rudolph

Publications and source records attributed to M Rudolph.

At least 55 records · Page 3Linked to original sources
PubMed1990

Direct enantiomeric separation of saterinone by high-performance liquid chromatography.

A new method is described for the direct liquid chromatographic separation and determination of the enantiomers of saterinone, 1-[(4-cyano-1,2-dihydro-6-methyl-2-oxopyridinyl-5)-phenoxy]-3-[4-( 2- methoxyphenyl)piperazinyl-1]-propan-2-ol, using a Chiralcel OD column with methanol as eluent and a temperature set to 10 degrees C. Several lots of synthesized enantiomers were analysed for their enantiomeric purity of more than 99%, because of the excellent resolution (Rs = 2.2). Furthermore the enantiomeric ratio was determined in plasma samples after oral and intravenous administration of racemic saterinone.

Administration, Oral↗
PubMed1990

[Fine structure studies and identification of lectin receptors on the viral envelope of two HIV-isolates].

The electron microscopic particle findings were compared with the levels of revertase in corresponding samples over a longer period of time, and a good correlation was found. Comparative investigations of the fine-structure of two HIV isolates did not reveal any morphological differences. It can be assumed, on the basis of the comparative studies on lectin receptors using Helix pomatia lectin, that the viral envelopes of the two isolates are equipped similarly with N-acetyl-d-galactosamine. The differences are not significant with mature particles.

Animals↗
PubMed1989

[Importance of radiographic study in diagnosis of temporomandibular pathology].

The X-ray survey is an important complement in the diagnosis of the temporomandibular pathology, anyone be the modality of it. The complexity of the articular structure origins difficulties in order to obtain an objective analysis of them, and the presence of radio-transparent articular elements is another factor attempting against the radiological study. This X-ray survey must be realized by experimented professional in an multidisciplinary equipment in the temporomandibular management.

Humans↗
PubMed1989

[Effect of tumor-promoting phorbol esters on retrovirus-infected human cells: scanning electron microscopy studies].

The effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) on cultivated human cells infected with a human cell line derived retrovirus were examined by using scanning electron microscopy. The most prominent immediate morphological changes were the rounding up of cell surfaces, an enhanced formation of blebs and an increasing tendency to cell detachment. The production of virus particles under TPA treatment was markedly augmented, reaching 300 to 500 particles in individual cells. The production of particles appeared nearly random over the cell surface.

Cell Line↗
PubMed1988

Analysis of hamster lymphomas for the presence of hamster papovavirus DNA.

The hamster papovavirus (HaPV) is a polyomavirus isolated from skin epitheliomas arising spontaneously in young Syrian hamsters. It can induce lymphomas and leukaemias in newborn hamsters. Although no virus particles are detectable by electron microscopy, high amounts of monomeric and oligomeric forms of extrachromosomal HaPV DNA molecules are found in the lymphoma cells. These molecules display deletions of about 300 nucleotides in length. Their role in the lymphoma induction is discussed.

Animals↗
PubMed1987

Determination of carazolol in tissues of pigs by high-performance liquid chromatography.

A new and sensitive method is described for the determination of the beta-receptor blocker Carazolol, 4-(2-hydroxy-3-isopropylaminopropoxy)-carbazole, in different animal tissues. The procedure comprises extraction of Carazolol from tissue, clean-up by Kieselgel adsorption and high-performance liquid chromatographic separation with fluorimetric detection. The determination limit is 0.48 microgram/kg sample. The method has been verified by measuring Carazolol in liver, kidney and filet of a fattening pig treated with Carazolol before slaughtering.

Adrenergic beta-Antagonists↗
PubMed1987

Effective propagation of various type D retroviruses in human lymphoblastoid Raji cells.

Type D retroviruses belong to viruses which often have a low titer in virus-reproducing cell-systems. In search of better conditions for virus propagation, Raji cells (an EBV-genome carrying human B-cell line) were infected with different isolates of type D retroviruses. as demonstrated by electron microscopy all of the viruses showed a very good and stable virus production during long term cultivation on Raji cells.

B-Lymphocytes↗
PubMed1986

Chemical crosslinking of lipids and proteins within Mason-Pfizer monkey and squirrel monkey type D retroviruses.

Nearest neighborhoods between lipids and proteins were investigated in Mason-Pfizer monkey virus (MPMV) and squirrel monkey retrovirus (SMRV) using the chemical crosslinking reagent dimethyl suberimidate (DMS). Analysis of DMS-induced lipid-protein complexes by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that in both viruses the major internal structural protein, p27 (MPMV) and p35 (SMRV), is linked to the lipid bilayer. Additionally, in SMRV a second protein (p20) was found to have a vicinal relationship to the lipid bilayer of the virus. Electron microscopic investigations of MPMV and SMRV particles indicated that, in accord with the crosslinking data, the core shell of these type D retroviruses is in close proximity to the viral membrane.

Animals↗
PubMed1985

Electron microscopic investigation on the fine structure of the virus of enzootic bovine leukosis (BLV).

Systematically electron microscopical control of BLV producing cells and stimulated lymphocytes of leukotic cattle in the last years revealed maturation processes of BLV in a different form from comparable type C viruses. Electron microscopic representation of bridge-like junctions between core and envelope within examined retroviruses were discussed and compared with new model concepts.

Animals↗
PubMed1985

Monoclonal antibodies against tumour-associated antigens: mycoplasma as a major technical obstacle and its possible circumvention by azaserine selection medium.

In experiments aimed at generating monoclonal antibodies against tumour associated antigens, mice are usually immunized with cancer cells (or membrane fractions prepared from it) obtained from surgery or cultured in vitro. In both situations there is the danger of introducing mycoplasmas into the hybridoma cultures even if the myeloma cell line and the mice used for immunization or feeder cell preparation are not infected. Mycoplasmas kill hybridoma cells during HAT selection because they excessively degrade thymidine. The use of azaserine instead of aminopterin in hybrid selection circumvents the necessity for thymidine and therefore may allow survival and growth of hybridomas in the presence of mycoplasmas.

Animals↗
PubMed1983

A simple method for purification of retroviruses using polyacrylnitrile.

A simple method has been developed that allows the concentration and partial purification of retroviruses from both small and large volumes of culture fluid in a comparatively short period of time. The method is based on the property of polyacrylnitrile (PAN) to adsorb preferentially nonviral proteins. Retroviruses purified by this procedure exhibit rather high specific reverse transcriptase activities and a good appearance in electron micrographs and protein patterns.

Acrylic Resins↗
PubMed1983

Electron microscopic evidence for parainfluenza type III/retrovirus phenotypically mixed particles.

Sublines of malignant permanent human fibroblast cell lines were shown to be infected by a type-D retrovirus (PMFV) and by a parainfluenza virus type III in an earlier study (2, 6). Careful electron microscopical investigations of these sublines have proved "viral structural elements" of both viruses in the same cell. We obtained electron microscopic evidence--though rarely--for parainfluenza type III/PMFV mixed-particles.

Cell Line↗
PubMed1983

Immortalization of human Lesch-Nyhan-fibroblasts following infection with Simian virus 40.

Skin fibroblasts of three Lesch-Nyhan patients were successfully transformed to unrestricted growth by Simian virus 40. The transformed character of isolated clones was confirmed by their epitheloid growth in medium containing low concentration of serum, by hyperdiploid karyotype patterns, by detection of SV40 T-antigen, by their growth in soft agar and by the proliferation of cells in vitro up to 200 passages hitherto.

Animals↗