Biomedical subjects

M Rusli

Publications and source records attributed to M Rusli.

3 recordsLinked to original sources

PubMed1987

Endotracheal diazepam: absorption and pulmonary pathologic effects.

We conducted a study to evaluate the absorption of endotracheally administered diazepam and the pulmonary pathologic changes induced by its administration. Six cats received diazepam and five cats received saline endotracheally. Serial blood gases and serum diazepam levels were drawn at intervals for 90 minutes after the administration of diazepam. The cats were sacrificed after two days and their lungs were examined by a pathologist. Mean diazepam levels reached a peak two minutes after the administration of diazepam and remained elevated above therapeutic levels for 90 minutes. There was no significant change in pH, PO2, or PCO2 for either group. Histologic examination of the lungs showed a significantly increased incidence of pneumonitis in the diazepam group as compared to the saline group. This study demonstrates that although diazepam is well absorbed when administered endotracheally, it has adverse effects on the lungs that may preclude endotracheal use in the currently available commercial form.

Absorption↗

PubMed1986

Cimetidine treatment of recalcitrant acute allergic urticaria.

Cimetidine, an H-2-receptor-blocking antihistamine, was used in the treatment of five consecutive patients who developed acute allergic urticaria in response to insect stings. All five had persistent hives after treatment with at least epinephrine and diphenhydramine (Benadryl, an H-1 receptor blocker). All also had been given corticosteroids. Each patient responded dramatically within 15 minutes to one IV infusion of 300 mg cimetidine. All were discharged on oral cimetidine (300 mg every six hours) and reported no recurrences or exacerbations of urticaria on follow up. There were no complications. Cimetidine may be effective in patients with recalcitrant acute allergic urticaria who are already being treated with H-1-receptor-blocking antihistamines. Controlled clinical trials should be done to confirm the efficacy of cimetidine for treatment of acute allergic reactions.

Adolescent↗

PubMed1977

Characterization of the nuclear envelope, pore complexes, and dense lamina of mouse liver nuclei by high resolution scanning electron microscopy.

We have used high resolution scanning electron microscopy (SEM) to study the nuclear envelope components of isolated mouse liver nuclei. The surfaces of intact nuclei are covered by closely packed ribosomes which are distinguishable by SEM from nuclear pore complexes. After removal of nuclear membranes with the nonionic detergent Triton X-100, the pore complexes remain attached to an underlying, peripheral nuclear lamina, as described by others. The surface of this dense lamina is composed of particulate granules, 75-150 A in diameter, which are contiguous over the entire periphery. We did not observe the pore-to-pore fibril network suggested by other investigators, but such a structure might be the framework upon which the dense lamina is formed. Morphometric analysis of pores and pore complexes shows their size, structure, and density to be similar to that of other mammalian cells. In addition, several types of pore complex-associated structures, not previously reported by other electron microscope (EM) techniques, are observed by SEM. Our studies suggest that the major role of the dense lamina is associated with the distribution, stability, and perhaps, biogenesis of nuclear pore complexes. Treatment of isolated nuclei with a combination of Triton X-100 and sodium deoxycholate removes membranes, dense lamina, and nuclear pore complexes. The resulting "chromatin nuclei" retain their integrity despite the absence of any limiting peripheral structures.

Animals↗