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M S Bektemirova

Publications and source records attributed to M S Bektemirova.

17 recordsLinked to original sources
PubMed1983

Rabies vaccine prepared from the virus grown in Japanese quail embryo cell cultures.

Fixed rabies virus strain MNIIVP-74 was grown in Japanese quail embryo cell cultures, concentrated by ultrafiltration and inactivated with beta-propiolactone. The resulting vaccine was markedly antigenic and immunogenic for laboratory animals. Human volunteers injected with 2.0 ml vaccine on days 0, 3, 7, 14, 30 and 90 exhibited more intensive and longer antibody production than those injected daily for 14 days.

Animals↗
PubMed1981

Comparative study of rabies virus persistence in human and hamster cell lines.

Persistent infections by rabies virus in BHK-21/13S and HEp-2 cells were studied comparatively. No evidence of interferon production, selection of virus-resistant cells, or integration of the viral genome could be found. Persisting viruses replicated efficiently at 34, 36, and 40 degrees C. Both persistently infected cultures released defective interfering virus particles. A cyclical pattern of infection, which was not characteristic of the persistently infected HEp-2 system, was observed in persistently infected BHK cultures. The virus from persistently infected BHK cultures lost its virulence for mice, whereas the virus from persistently infected HEp-2 cultures retained mouse-killing capacity for more than 3 years.

Animals↗
PubMed1979

Properties of rabies virus (MNIIVP-74 strain) adapted to Japanese quail embryo cell culture.

The Pasteur strain of fixed rabies virus was adapted to primary cell cultures of Japanese quail embryos and designated as MNIIVP-74. In the course of adaptation the virus pathogenicity for rabbits by the intracerebral route decreased considerably and the pathogenicity for rabbits and adult white mice by extraneural routes was completely lost. After inoculation of Japanese quail embryo cell cultures, a titer of the virus in the culture fluid at 4 days was 6.25--7.0 lg LD50/ml (by the intracerebral inoculation of adult white mice). Viral antigen could be detected by immunofluorescence in the cytoplasm of approximately 60 per cent of the cells. Virus multiplication was accompanied by intensive interferon production. In cultures of BHK-21/13S cells the titer of the virus reached was 5.75 lg LD50/ml at 24 hours and about 30 per cent of the cells were affected. The MNIIVP-74 virus showed a high immunogenic activity in rabbits, guinea pigs and mice.

Animals↗
PubMedDate not supplied

[Effect of host cells on the course of chronic rabies virus infection in cell cultures].

Different patterns of rabies virus infection were observed in BHK-21/13S and HEp-2 cell cultures at late stages of persistent infections. The infection of BHK-21/13S cells was characterized by periodical increases and declines in the portion of the antigen-containing cells (from 100% to less than 1%) and low infectivity titers which did not correspond to fluctuations in the antigen production and occasional resistance to challenge with vesicular stomatitis virus. In contrast, persistently infected HEp-2 cell culture exhibited a more constant course of antigen production which never reached extreme points; the infectivity titers generally correlated with the portion of the antigen-containing cells. Cultivation in the presence of antirabies serum did not "cure" either of the cultures.

Antigens, Viral↗
PubMedDate not supplied

[Chronic cell culture infection with the rabies virus].

Three cell cultures chronically infected with fixed rabies virus, strain MNIIVP-74, have been obtained: HEp-2/2, BHK/13S, and RK-13. In the former two cultures, the infectious virus titers were 2.0 to 5.25 Ig LD50/ml. In RK-13 cells, traces of the infectious virus were found. In the chronically infected HEp-2/2 culture the maximum amount of the antigen-containing cells determined by the fluorescent antibody procedure was 60% and in BHK/13S 80%. Chronically infected cultures had a reduced growth rate and were as sensitive as the controls of superinfection with vesicular stomatitis virus. The virus recovered from chronically infected culture produced a disease in mice at later intervals than the original virus used in the same doses.

Animals↗
PubMedDate not supplied

[Reproduction of fixed rabies virus in a quail fibroblast culture growing in suspension].

Reproduction of culture variant of fixed rabies virus was studied in cultures of quail fibroblasts growing in roller suspension cultures, as well as in monolayer cultures of Japanese quail embryos fixed on DEAE-Sephadex A-50 particles. Titers of the virus in cultures on the microcarrier were found to be similar with those in roller monolayer cultures, namely 5.75--6.5 lg LD50/ml. In shaker and roller suspension cultures the virus titers were lower: 4.5--5.25 lg LD50/ml.

Animals↗
PubMedDate not supplied

[Properties of the rabies virus long persisting in cell cultures].

Properties of rabies virus (RV) persisting in chronically infected cultures (CIC): HEp-2--RV and BHK-RV were studied. RV from the HEp-2-RV system retained its pathogenicity for mice for more than 3 years of observation. RV from the BHK-RV system lost this property after 50 passages in CIC. Both RV variants from CIC formed plaques in CER cells, showed marked immunogenic activity in mice, had no interferon-inducing activity in BHK-21 cells, and were not temperature-sensitive. Electron microscopic examinations of CIC culture fluids showed virions of bullet-like, oval, or spherical shapes.

Animals↗
PubMedDate not supplied

[Comparative study of the persistence of 3 RNA-containing viruses in a continuous cell culture of human origin].

A comparative study of chronic infecton of HEp-2 cells with tick-borne encephalitis (TBE), rabies (RV), and rubella (RuV) viruses was carried out. Throughout the entire period of chronic infection (CI) no signs of specific cell destruction by these viruses were observed. The infectious virus was regularly demonstrated in the culture fluid and chronically infected cells. The antigenic properties of the persisting viruses did not differ from those of the original strains. The persisting TBE and rabies viruses replicated in the susceptible cells at a higher temperature and formed plaques of a smaller size than the original virus. The number of chronically infected cells producing infectious virus was always less than the number of cells containing the virus-specific antigen. In all three types of chronic infection the cells supported virus persistence at 40 degrees C. In TBE and RuV chronically infected cells interference with heterologous viruses was marked while in HEp-2-RV homologous interference caused by formation of defective interfering particles was observed. Treatment of the cells with BUDR resulted in activation of the infection only in the HEp-2-TBE system. Experiments on transfection of the sensitive cells by using DNA from HEp-2-RV and HEp-2-RuV gave negative results. The importance of various factors in the mechanism of virus persistence in the chronically infected cells under study is discussed.

Animals↗
PubMedDate not supplied

[Factors responsible for the persistence of rabies virus in cell cultures].

Persistence of rabies virus (RV) was studied in two cell cultures: HEp-2-RV and BHK-RV. The infection was demonstrated in 8% cells of the BHK-RV system and 53% cells of HEp-2-RV system by the method of cloning. The virus-free clones were as sensitive to RV infection as the control cultures. The incubation temperature shifts up to 40 degrees C and down to 34 degrees C did not affect the carrier state. 5-BUDR did not activate the infectious process in the chronically infected cells. The factors possibly responsible for RV persistence in cell cultures are discussed.

Animals↗