[Pathomorphology of brain microcirculatory disorders in asphyxia and pneumonias in newborn and young infants].
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Biomedical subjects
Publications and source records attributed to M S Brumshteĭn.
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A method for urgent histocytological examination of the internal organs at autopsies and urgent biopsies is offered which is a useful addition to the routine histological examination. The method is based on the preparation of an emulsion from the organ tissue, staining it without preliminary fixation, and examination after pressing with a cover slip or in dried smears after poststaining. This method of specimen treatment is simple, time-saving, needs no freezing microtome. Preparations can be made in 3-5 minutes.
To ascertain the occurrence of disorders of blood circulation in the heart, in particular, in acute impairment of the coronary blood circulation and repeated infarctions, perfusion of the vascular system of the heart with a solution of a stain was carried out. It was shown that those parts of the heart where, due to certain reasons, there were created conditions for development of impairment of the microcirculation, remained unstained. It was corroborated by the results of histological and cytological investigations of the myocardium. The coincidence of clinical observations with the results of the perfusion may indicate that this method of investigation of the heart at autopsy makes it possible to understand better prevital disorders of capillary blood circulation. The method of perfusion of the heart with a stain is set forth in the work in detail.
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